Impact of Neoadjuvant Therapy on PD-L1 Expression in Triple-Negative Breast Cancer and Correlation with Clinicopathological Factors.

Background: This study aims to deliver more insights on the impact of neoadjuvant treatment on Pd-L1 expression and to evaluate its correlation with clinicopathological factors.

Methods: We reviewed 88 TNBC cases for the period 2021-2023. Data on age, tumor size, stage, and treatment were collected.

Black Chokeberry () Juice Supplementation Improves Oxidative Stress and Aging Markers in Testis of Aged Rats.

Spermatogenesis is a process that continues until the end of an individual's life, although with reduced activity with advancing age. Inflammation, oxidation, and apoptosis are events considered as predictors of pathogenesis and the development of age-related diseases observed in aged testes. The use of natural compounds with antioxidant and anti-inflammatory properties has a beneficial effect on the inflammatory and oxidative status of the aged testis.

Diet-Induced Early Inflammatory Response of Visceral Adipose Tissue in Healthy Male Wistar Rats.

The prolonged consumption of a high-fat diet (HFD) leads to abnormal growth of the visceral adipose tissue (VAT), increased macrophage infiltration, and altered secretion of biologically active molecules. This is considered as a precondition for the development of obesity, inflammation, and obesity-related disorders. Therefore, we studied HFD-induced changes in the tissue levels of the inflammatory markers C-reactive protein, serum amyloid-A, and interleukin-4 in healthy male Wistar rats.

Antibacterial Action of Protein Fraction Isolated from Hemolymph against NBIMCC 8785.

Natural products and especially those from marine organisms are being intensively explored as an alternative to synthetic antibiotics. However, the exact mechanisms of their action are not yet well understood. The molecular masses of components in the hemolymph fraction with MW 50-100 kDa from were determined using ImageQuant™ TL v8.

High lipid diet and the expression of proinflammatory markers in testis.

Abstract.

Fruit Juice Modulates ACE2 Immunoexpression and Diminishes Age-Related Remodeling of Coronary Arteries in Rats.

The aim of the study is to evaluate the effect of fruit juice (AMJ) supplementation on age-related coronary arteries remodeled in aged rat hearts. Male Wistar rats (n = 24) were divided into three groups: (1) young controls (CY), aged 2 months, without AMJ supplementation; (2) old controls (CO), aged 27 months, without AMJ supplementation; and (3) the AMJ group (A), which used 27-month old animals, supplemented orally with AMJ for 105 days. AMJ supplementation did not influence the wall-to-diameter parameter (Kernohan index) of the coronary arteries of test animals.

Effect and Mechanisms of Antibacterial Peptide Fraction from Mucus of against NBIMCC 8785.

Peptides isolated from the mucus of Cornu aspersum could be prototypes for antibiotics against pathogenic bacteria. Information regarding the mechanisms, effective concentration, and methods of application is an important tool for therapeutic, financial, and ecological regulation and a holistic approach to medical treatment. A peptide fraction with MW < 10 kDa was analyzed by MALDI-TOF-TOF using Autoflex™ III.

Microbial diversity of garden snail mucus.

The search for new natural compounds for application in medicine and cosmetics is a trend in biotechnology. One of the sources of such active compounds is the snail mucus. Snail physiology and the biological activity of their fluids (especially the mucus) are still poorly studied.

Black Chokeberry () Functional Beverages Increase HDL-Cholesterol Levels in Aging Rats.

Plant-based foods rich in phenolic phytochemicals are among the promising strategies to counteract age-related changes in lipid profile. (AM) fruits are a rich source of phenolic compounds possessing lipid-modulating effects. The present study investigated the effect of 3-month supplementation of AM-based functional beverages on the lipid profile of healthy aging rats.

Antimicrobial Activities of Different Fractions from Mucus of the Garden Snail .

Natural products have long played a major role in medicine and science. The garden snail is a rich source of biologically active natural substances that might be an important source for new drugs to treat human disease. Based on our previous studies, nine fractions containing compounds with Mw <3 kDa; <10 kDa; <20 kDa; >20 kDa; >30 kDa>50 kDa and between 3 and 5 kDa; 5 and 10 kDa; and 10 and 30 kDa were purified from the mucus of and analyzed by tandem mass spectrometry (MALDI-TOF/TOF).

Impact of a High-fat Diet on the Development of Chronic Inflammation in Heart of Wistar rats.

Introduction: Obesity is linked to the development of low-grade, chronic inflammation. Obesity-related inflammation appears to be a different type of inflammation, mainly due to excessive food intake and unusual homeostasis. It can be evaluated by measuring the concentration of pro- and anti-inflammatory marker molecules – C-reactive protein (CRP), serum amyloid-A (SAA) and interleukin-4.

Aronia melanocarpa (Michx.) Elliot fruit juice reveals neuroprotective effect and improves cognitive and locomotor functions of aged rats.

The aim of the study was to investigate the effect of polyphenol-rich Aronia melanocarpa (Michx.) Elliot juice (AMJ) on learning ability and memory, and brain morphology of aged rats. A model of healthy male Wistar rats (24 months of age) divided in 2 groups was used: AMJ group supplemented orally with AMJ (10 mL/kg for 105 days) and old control (CO) group without supplementation.

Chloroplast Genome Analysis of Resurrection Tertiary Relict Highlights Genes Important for Desiccation Stress Response.

is a paleolithic tertiary relict species, best known as a resurrection plant with remarkable tolerance to desiccation. When exposed to severe drought stress, shows an ability to maintain the structural integrity of its photosynthetic apparatus, which re-activates easily upon rehydration. We present here the results from the assembly and annotation of the chloroplast (cp) genome of , which was further subjected to comparative analysis with the cp genomes of closely related species.

Comparison of the Influence of Nanodiamonds and Single-Walled Nanotubes on Phenol Biodetoxification by Pseudomonas sp.

Nanobiotechnologies are a rapidly growing field that offers new opportunities thus far unknown including regulation processes at a nano level. The biodetoxification and mechanisms of degradation of many xenobiotics have been studied and are well documented. There remains the important issue of the impact of nanomodulators on biodetoxification processes and their potential to optimize and regulate biodegradation of recalcitrant xenobiotics.

Antiatherogenic and Cardioprotective Effects of Black Chokeberry (Aronia melanocarpa) Juice in Aging Rats.

Age-related diseases are a social problem of global significance and their prevention by natural products is a research area of particular interest. The present study is an approach to counteract the risk factors for atherosclerosis arising in the aging process by supplementation of chokeberry juice. It employed a model of healthy adult rats monitored for a number of somatometric, serum lipidogram, and histopathological parameters, related to risk factors and their response to supplementation with antioxidant-rich chokeberry juice.

Modelling of risk effect of mercury on nutrient transformation in lake sediments.

The influence of mercury on the transformation processes in the waters and the sediments in the reservoir of a small hydroelectric power plant (SHPP) Lakatnik was simulated in laboratory conditions. SHPP Lakatnik is the first (of nine similar) SHPPs put into exploitation in the middle part of the Iskar River (Bulgaria). In previous studies of the sediments in the reservoir of SHPP Lakatnik, our team found high concentrations of mercury, sometimes exceeding maximum concentration limit (MCL).

Computational identification of novel microRNA homologs in the chimpanzee genome.

MicroRNAs are important negative regulators of gene expression in higher eukaryotes. The miRNA repertoire of the closest human animal relative, the chimpanzee (Pan troglodytes), is largely unknown. In this study, we focused on computational search of novel miRNA homologs in chimpanzee.

[Our experience in using the IP-2-50 infusion pump with newborn infants, pregnant women and puerperae].

The authors describe indications for usage of venous administrations by means of the infusion pump IP 2-50 in newborns, pregnant women and parturients as well as during feeding of the smallest preterm infants with the same pump. Advantages and disadvantages of IP 2-50 are presented in comparison with the perfusion pumps "Braun Melsungen" and "Lineomat". Nosologic units are described, in which infusions with IP 2-50 are applied.

Alkaloids of Corydalis slivenensis.

The known alkaloids (-)-stylopine, (-)-canadine, (+/-)-sinactine, (+)-nantenine, (+)-bulbocapnine, protopine, (-)-isocorydine, (-)-domesticine, alpha-allocryptopine and berberine were found in a mixture of alkaloids from Corydalis slivenensis (Papaveraceae), growing in Bulgaria. Dehydronantenine, corydine, predicentrine and isoboldine were detected by means of a thin-layer chromatography. Two other alkaloids - (+)-tetrahydrocorysamine and (-)-cavidine, were identified on the basis of their spectral data.