Massively parallel characterization of transcriptional regulatory elements.

The human genome contains millions of candidate cis-regulatory elements (cCREs) with cell-type-specific activities that shape both health and many disease states. However, we lack a functional understanding of the sequence features that control the activity and cell-type-specific features of these cCREs. Here we used lentivirus-based massively parallel reporter assays (lentiMPRAs) to test the regulatory activity of more than 680,000 sequences, representing an extensive set of annotated cCREs among three cell types (HepG2, K562 and WTC11), and found that 41.

Hepatoprotective efficacy of Argemone mexicana L. root in paracetamol-induced hepatotoxicity in a rat model.

Ethnopharmacological Relevance: Argemone mexicana L. (Papaveraceae), a weed that thrives in the tropical and subtropical areas of South and Central America, Mexico, Caribbean Islands and India. In India, it has been used traditionally to treat vesicular calculus, inflammatory conditions, and hepatobiliary disorders.

Effects of Elective Percutaneous Coronary Intervention on Subtle Left Ventricular Systolic Dysfunction in Patients With Stable Coronary Artery Disease as Assessed by Global Longitudinal Strain Imaging.

Aim The study aimed to detect subtle left ventricular (LV) systolic dysfunction, reflected by abnormal global longitudinal strain (GLS), in patients with stable coronary artery disease (CAD) undergoing percutaneous coronary intervention (PCI) and to evaluate any improvement in GLS at 24 hours and six months post-PCI. Methods A total of 94 patients with stable CAD scheduled for elective PCI at our hospital were evaluated using conventional 2D echocardiography and GLS prior to the procedure. Follow-up assessments were conducted at 24 hours and six months post-PCI.

Integrating machine learning and remote sensing for long-term monitoring of chlorophyll-a in Chilika Lagoon, India.

Chlorophyll-a (Chla) is recognized as a key indicator of water quality and ecological health in aquatic ecosystems, offering valuable insights into ecosystem dynamics and changes over time. This study aimed to to develop and validate a robust ML model for estimating Chla using Landsat data, produce a time series of Chl a maps, and analyze the spatiotemporal variability of Chla in Chilika Lagoon, Asia's largest brackish water lagoon. Nine ML regression models, including Extreme Gradient Boost, Support Vector Regression, Random Forest, and Bagging Regression, were evaluated using Landsat imagery and field data.

Microwave-Assisted Synthesis of Platinum-free High-Entropy Alloy Catalysts on Reduced Graphene Oxide Sheets for Enhanced Oxygen Reduction and Evolution Reactions.

Nano-sized high-entropy materials (HEMs) recently received more attention to researchers due to their superior electrochemical catalytic properties. HEMs comprise at least five elements with or without metals and are synthesized through solid-state reactions and solution-mediated techniques. The presence of many elements in these HEMs result in a high mixing entropy and facilitates the formation of stable solid solutions in fundamental crystal structures.

Key epigenetic and signaling factors in the formation and maintenance of the blood-brain barrier.

The blood-brain barrier (BBB) controls the movement of molecules into and out of the central nervous system (CNS). Since a functional BBB forms by mouse embryonic day E15.5, we reasoned that gene cohorts expressed in CNS endothelial cells (EC) at E13.

Solar Assisted Mitigation of Chloramphenicol and H Evolution Using CuNi Alloy Nanoparticles on h-BN Doped g-CN: A Comprehensive Approach Combining Synchrotron and DFT Analysis.

A simple one-step deposition-precipitation method was used to synthesize highly active and well-defined CuNi alloy bimetallic nanoparticles supported on h-BN/g-CN. The nanocomposite was applied for hydrogen gas evolution via seawater splitting and photocatalytic chloramphenicol (CHP) removal. Through TEM and synchrotron studies, the formation of CuNi alloy and uniform distribution of CuNi bimetallic nanoparticles on the h-BN/g-CN surface was observed.

Therapeutic Potential of Neutralizing Monoclonal Antibodies (nMAbs) against SARS-CoV-2 Omicron Variant.

Background: The COVID-19 pandemic has spurred significant endeavors to devise treatments to combat SARS-CoV-2. A limited array of small-molecule antiviral drugs, specifically monoclonal antibodies and interferon therapy, have been sanctioned to treat COVID-19. These treatments typically necessitate administration within ten days of symptom onset.

PIM1 kinase and its diverse substrate in solid tumors.

The PIM kinase family, consisting of PIM1, PIM2, and PIM3, is a group of serine/threonine protein kinases crucial for cellular growth, immunoregulation, and oncogenesis. PIM1 kinase is often overexpressed in solid and hematopoietic malignancies, promoting cell survival, proliferation, migration, and senescence by activating key genes. In vitro and in vivo studies have established the oncogenic potential of PIM1 kinases.

Unveiling the interaction, cytotoxicity and antibacterial potential of pyridine derivatives: an experimental and theoretical approach with bovine serum albumin.

The binding interactions between bovine serum albumin (BSA) and three pyridine derivatives, i.e., 2-(5-bromopyridin-3-yl) acetic acid (L1), 3-bromo-5-nitropyridine (L2) and 2-chloro-4-nitropyridine (L3), have been carried out using UV-Vis and fluorescence spectroscopic methods.

Assessment of oral health status amongst postal employees of Bhubaneswar city: A cross-sectional study.

Introduction: Upholding good dental health has wider effects on productivity and performance at work in addition to being crucial for individual well-being. Because it can affect both their personal well-being and productivity at work, post office workers' oral health is therefore a critical issue of concern. Hence, evaluating Bhubaneswar city post office workers' dental health is the study's aim.

A doxorubicin loaded chitosan-poloxamer implant for the treatment of breast cancer.

Breast cancer is a serious concern for many women worldwide. Drug-loaded implants have shown several benefits over systemic administrations. To provide anti-cancer drugs with controlled release and reduced systemic toxicity, biodegradable implants have attracted a lot of attention.

A Schiff-Base Molecular Probe for Selective Fluorescence Sensing of Maleic Acid with Recognition of Maleic Acid in Food Additives and Cell Imaging.

The 2,4-dinitrophenylhydrazine based Schiff base (L) acts as an effective fluorescence sensor for the selective detection of maleic acid. The detection limit of L towards maleic acid is observed to be 1.29 × 10 M.

Near-Infrared Driven Gold Nanoparticles-Decorated g-CN/SnS Heterostructure through Photodynamic and Photothermal Therapy for Cancer Treatment.

Background: Phototherapy based on photocatalytic semiconductor nanomaterials has received considerable attention for the cancer treatment. Nonetheless, intense efficacy for in vivo treatment is restricted by inadequate photocatalytic activity and visible light response.

Methods: In this study, we designed a photocatalytic heterostructure using graphitic carbon nitride (g-CN) and tin disulfide (SnS) to synthesize g-CN/SnS heterostructure through hydrothermal process.

Protective effect of Tecoma stans (L.) Juss.ex Kunth in CFA-induced arthritic rats.

Ethnopharmacological Relevance: Tecoma stans (L.) Juss.ex Kunth (Bignoniaceae) is mainly found in tropical and subtropical regions of Africa and Asia.

Unveiling Fluorescence Spectroscopy, Molecular Docking and Dynamic Simulations: Interactions Between Protein and 2, 4-Dinitrophenylhydrazine Schiff Base.

In this study, we aimed to explore the interaction mechanism between bovine serum albumin (BSA) and a Schiff base compound derived from 2,4-dinotrophenyl hydrazine (L) using various spectroscopic techniques. The interaction between BSA and synthesizing molecule can provide insights into binding affinity, conformational changes and potential applications in drug delivery or biochemistry. The interaction between BSA and L was studied by using UV-Vis and fluorescence titration analysis.

Construction of Methotrexate-Loaded BiS Coated with Fe/Mn-Bimetallic Doped ZIF-8 Nanocomposites for Cancer Treatment Through the Synergistic Effects of Photothermal/Chemodynamic/Chemotherapy.

A combination of therapeutic modalities in a single nanostructure is crucial for a successful cancer treatment. Synergistic photothermal therapy (PTT) can enhance the effects of chemodynamic therapy (CDT) and chemotherapy, which could intensify the therapeutic efficacy to induce cancer cell apoptosis. In this study, Fe and Mn on a zeolitic imidazolate framework (ZIF-8) (Fe/Mn-ZIF-8; FMZ) were synthesized through ion deposition.

The evolving pathophysiology of TBI and the advantages of temporally-guided combination therapies.

Several clinical and experimental studies have demonstrated that traumatic brain injury (TBI) activates cascades of biochemical, molecular, structural, and pathological changes in the brain. These changes combine to contribute to the various outcomes observed after TBI. Given the breadth and complexity of changes, combination treatments may be an effective approach for targeting multiple detrimental pathways to yield meaningful improvements.

A Novel Web-Based Approach for Monitoring Biodiversity.

Understanding complexities in biodiversity is one of the fundamental goals of ecology and its monitoring is significant for ecosystem sustainability, maintenance, and conservation. However, biodiversity monitoring needs improvement to handle complex datasets and their analyses. This study attempts to understand these ecological complexities quickly, efficiently, and easily.

Assessment of Oral Health Status among the Sevayats of Shree Sakhigopal Temple, Puri - A Cross-sectional Study.

Aim: To assess the oral health status of the Sevayats of Sakhigopal temple, Puri, Odisha.

Methodology: An assessment of the oral health status of 111 Sevayats at the Sakhigopal temple in Puri was carried out using a cross-sectional survey. The World Health Organization oral health assessment form for adults (2013) was used to collect data.