Simultaneous detection of dengue virus serotypes in a dual-serotype-detection nucleic acid based lateral flow assay.

Dengue virus (DENV) is an important arthropod-borne viral disease, with four antigenically and genetically diverse serotypes (DENV-1, DENV-2, DENV-3, and DENV-4). Timely and accurate diagnosis of dengue virus serotypes is crucial for the management of outbreaks. This study focussed on the development of a RT-PCR based lateral flow strip assay to detect DENV serotypes in a dual detection manner without using gel electrophoresis.

The complexity of kodo millet: genomic analysis and implications in crop improvement.

This article explores possible future initiatives, such as the development of targeted breeding and integrated omics approach to boost kodo millet production, nutritional value, and environmental adaptation. Kodo millet is grouped under the genus Paspalum and family Gramineae. It is a tropical African crop that was initially domesticated in India approximately 3000 years ago.

Engineering considerations in the design of tissue specific bioink for 3D bioprinting applications.

Over eight million surgical procedures are conducted annually in the United Stats to address organ failure or tissue losses. In response to this pressing need, recent medical advancements have significantly improved patient outcomes, primarily through innovative reconstructive surgeries utilizing tissue grafting techniques. Despite tremendous efforts, repairing damaged tissues remains a major clinical challenge for bioengineers and clinicians.

Infectious etiology of intussusception in Indian children less than 2 years old: a matched case-control analysis.

Background: Enteric infections are hypothesized to be associated with intussusception in children. A small increase in intussusception following rotavirus vaccination has been seen in some settings. We conducted post-marketing surveillance for intussusception following rotavirus vaccine, Rotavac introduction in India and evaluated association of intussusception with enteric pathogens.

Microhaplotypes: the QR-code for Plasmodium vivax infection dynamics.

An array of microhaplotypes - small segments of ≤200 nucleotides with heterozygous multiple-SNP exhibiting multiple allelic combinations - were identified in the Plasmodium vivax genome by Siegel et al. Interestingly, the microhaplotype has significant potential to distinguish relapse/reinfection and identify genetic relatedness across vivax-endemic areas. It is essential to validate the universal applicability of microhaplotypes.

Fingerprints of El Niño Southern Oscillation on global and regional oceanic chlorophyll-a timeseries (1997-2022).

With the advancement of present-day ocean color satellites, the spatiotemporal variations of oceanic Chlorophyll-a (Chl-a) concentration, a proxy for phytoplankton population, can be monitored at regional and global scales. Estimating long-term changes in Chl-a concentration, however, is mainly constrained by the limited availability of ocean color data and the significant influence of climate oscillations like the El Niño Southern Oscillation (ENSO). In this study, we investigate the influence of ENSO on regional and global Chl-a timeseries using two ocean color datasets spanning from September 1997 to December 2022.

Distinct methylome profile of cfDNA in AMI patients reveals significant alteration in cAMP signaling pathway genes regulating cardiac muscle contraction.

Background: The role of epigenetics in cardiovascular diseases has paved the way for innovative therapeutic approaches. Investigating epigenetic changes using cell-free DNA (cfDNA) holds substantial promise beyond mere diagnostics, especially for heart-related conditions like acute myocardial infarction (AMI), where obtaining tissue samples is a challenge. This study explores the methylation patterns of cfDNA in AMI patients and compares them with genomic DNA (gDNA) from the same individuals, aiming to evaluate the effectiveness of cfDNA as a valuable resource for studying heart-related diseases.

Assessment of Long-Term Pulmonary Function and Quality of Life in Patients with Chronic Obstructive Pulmonary Disease (COPD).

Background: Chronic obstructive pulmonary disease (COPD) is a prevalent respiratory condition portrayed by persistent airflow limitation and correlated symptoms. Despite advances in treatment, COPD remains a significant global health burden, necessitating ongoing research to understand its long-standing impact on pulmonary function and quality of life (QoL).

Methods: This longitudinal research enrolled 200 COPD and followed them over a 5-year period.

Immunomodulation in dengue: towards deciphering dengue severity markers.

Background: Dengue is a vector-borne debilitating disease that is manifested as mild dengue fever, dengue with warning signs, and severe dengue. Dengue infection provokes a collective immune response; in particular, the innate immune response plays a key role in primary infection and adaptive immunity during secondary infection. In this review, we comprehensively walk through the various markers of immune response against dengue pathogenesis and outcome.

Progressive familial intrahepatic cholestasis 3 Camouflaging as Wilson disease in a 12-year-old: a diagnostic Odyssey.

Primary Familial Intrahepatic Cholestasis type 3 is an exceedingly rare genetic cholestatic disorder characterized by the defective hepatocanaliculr bile acid transport leading to progressive liver disease. In this case report, we describe the course of treatment for a 12-year-old kid diagnosed with Wilson disease based on Leipzig score and copper investigations. The child did not improve with chelation therapy and was subsequently genetically classified as PFIC-3.

A comparative thermo-chemical characterization of oilseed, millet and pulse stem biomass for bioethanol production.

Various thermochemical and biochemical processes are resorted to transform agri-wastes into diverse green fuels. Current investigation encompassed three different types of biomass gingelly, kodo millet and horse grams, whose desirability as biofuel feedstock have been largely unexplored till date. The existence of significant amount of cellulose (38.

Exploring Campus Configuration and Patient Arrival Behaviors at Large Hospitals: An Integrated Post-Occupancy Evaluation (POE) Protocol.

Background: This study addresses the challenges in hospital navigation, particularly focusing on the sequence of arrival experiences through foot traffic or driving within a medical campus. While previous research has emphasized wayfinding in interiors, this study recognizes the importance of transition from outdoor to indoor spaces in the hospital arrival zone.

Objectives: Twofold research efforts include: (1) Exploring vehicular and pedestrian behaviors in a hospital arrival zone using Space Syntax Analyses (SSA), and (2) Validating the SSA results using empirical data collected from onsite observations and behavior mapping.

Kappa Opioid Receptor Activation Induces Epigenetic Silencing of Brain-Derived Neurotropic Factor HDAC5 in Depression.

Treatment-resistant depression (TRD) occurs in almost 50% of the depressed patients. Central kappa opioid receptor (KOR) agonism has been demonstrated to induce depression and anxiety, while KOR antagonism alleviates depression-like symptoms in rodent models and TRD in clinical studies. Previously, we have shown that sustained KOR activation leads to a TRD-like phenotype in mice, and modulation of brain-derived neurotrophic factor (BDNF) expression in the prefrontal cortex (PFC) appears to be one of the molecular determinants of the antidepressant response.

Surface Engineered Osteoblast-Extracellular Vesicles Serve as an Efficient Carrier for Drug and Small RNA to Actively Target Osteosarcoma.

Osteosarcoma (OS) is a rare malignant tumor that affects soft tissue and has high rates of lung metastasis and mortality. The primary treatments for OS include preoperative chemotherapy, surgical resection of the lesion, and postoperative chemotherapy. However, OS chemotherapy presents critical challenges related to treatment toxicity and multiple drug resistance.

Differentially used codons among essential genes in bacteria identified by machine learning-based analysis.

Codon usage bias (CUB), the uneven usage of synonymous codons encoding the same amino acid, differs among genes within and across bacteria genomes. CUB is known to be influenced by gene expression and accordingly, CUB differs between the high-expression and low-expression genes in several bacteria. In this article, we have extended codon usage study considering gene essentiality as a feature.

Improvement of little millet (Panicum sumatrense) using novel omics platform and genetic resource integration.

This article explores possible future initiatives, such as the development of targeted breeding and integrated omics approach to boost little millet production, nutritional value, and environmental adaptation. Little millet (P. sumatrense) is a staple grain in many parts of Asia and Africa owing to its abundance in vitamins and minerals and its ability to withstand harsh agro-ecological conditions.

Optimized protoplast isolation and transfection with a breakpoint: accelerating Cas9/sgRNA cleavage efficiency validation in monocot and dicot.

Unlabelled: The CRISPR-Cas genome editing tools are revolutionizing agriculture and basic biology with their simplicity and precision ability to modify target genomic loci. Software-predicted guide RNAs (gRNAs) often fail to induce efficient cleavage at target loci. Many target loci are inaccessible due to complex chromatin structure.

Synergistic Interaction between Polysaccharide-Based Extracellular Matrix and Mineralized Osteoblast-Derived EVs Promotes Bone Regeneration via miRNA-mRNA Regulatory Axis.

Extracellular vesicles (EVs) derived from bone progenitor cells are advantageous as cell-free and non-immunogenic cargo delivery vehicles. In this study, EVs are isolated from MC3T3-E1 cells before (GM-EVs) and after mineralization for 7 and 14 days (DM-EVs). It was observed that DM-EVs accelerate the process of differentiation in recipient cells more prominently.

Revealing the hidden world of soil microbes: Metagenomic insights into plant, bacteria, and fungi interactions for sustainable agriculture and ecosystem restoration.

The future of agriculture is questionable under the current climate change scenario. Climate change and climate-related calamities directly influence biotic and abiotic factors that control agroecosystems, endangering the safety of the world's food supply. The intricate interactions between soil microorganisms, including plants, bacteria, and fungi, play a pivotal role in promoting sustainable agriculture and ecosystem restoration.

Assessment of Pulmonary Functions in Parkinson's Disease and Unveiling the Role of Levodopa Therapy: A Cross-Sectional Study.

Introduction: This investigation aimed to thoroughly characterize the range of pulmonary function abnormalities present in individuals with Parkinson's disease (PD) and to evaluate the effects of levodopa therapy on these respiratory dysfunctions.

Methods:  Ninety-five PD patients diagnosed via the UK Parkinson's Disease Society Brain Bank Diagnostic Criteria were recruited, excluding those with a smoking history or unable to perform pulmonary function tests (PFTs). Severity was assessed using the Hoehn and Yahr Scale.