Prognostic accuracy of lactate and procalcitonin in addition to national early warning score in patients with suspected sepsis - A cross-sectional study in a tertiary care center.

Background: Sepsis, a major global health concern, leads to millions of deaths annually, hence the need for early and reliable prognostic tools to assess patient risk and guide clinical decision making becomes crucial. This cross-sectional study evaluated the prognostic accuracy of integrating blood lactate and serum procalcitonin (PCT) levels with the National Early Warning Score (NEWS) for predicting mortality in sepsis patients. The objective was to assess whether this lactate and procalcitonin integrated with NEWS score (LP NEWS) could serve as a more effective early prognostic tool compared to established severity scores.

HCMV detection in Asian gastric cancer RNA-seq data sets and clinical validation in Indian GC patients reveals the HCMV-GC specific gene signatures.

Gastric cancer (GC) prevalence is very high in the Asian population. Oncogenic viruses play a crucial role in the progression of different types of cancers. Through reanalysis of clinical RNA-seq data sets derived from Asian GC patients, this study identified the presence of human cytomegalovirus (HCMV) in Asian GC tumors, next to the well-studied association of EBV.

Skp2-Cyclin A Interaction Is Necessary for Mitotic Entry and Maintenance of Diploidy.

Skp2, the substrate recognition component of the SCF ubiquitin ligase, has been implicated in the targeted destruction of a number of key cell cycle regulators and the promotion of S-phase. One of its critical targets is the Cyclin dependent kinase (Cdk) inhibitor p27, and indeed the overexpression of Skp2 in a number of cancers is directly correlated with the premature degradation of p27. Skp2 was first identified as a protein that interacts with Cyclin A in transformed cells, but its role in this complex has remained unclear.

Endogenous PTEN acts as the key determinant for mTOR inhibitor sensitivity by inducing the stress-sensitized PTEN-mediated death axis in KSHV-associated malignant cells.

As a part of viral cancer evolution, KSHV-infected human endothelial cells exert a unique transcriptional program upregulated mTORC1 signaling. This event makes them sensitive to mTOR inhibitors. Master transcriptional regulator PTEN acts as the prime regulator of mTOR and determining factor for mTOR inhibitory drug resistance and sensitivity.

Oxidation of biological molecules with age and induced oxidative stress in different growth phases of Saccharomyces cerevisiae.

One of the mechanistic approaches for explaining ageing is the oxidative stress theory of ageing. Saccharomyces cerevisiae has been used as a model to study ageing due to many factors. We have attempted to investigate if the differential ability to withstand oxidative stress can be correlated with their lifespans.

Aspect based sentiment analysis using multi-criteria decision-making and deep learning under COVID-19 pandemic in India.

The COVID-19 pandemic has a significant impact on the global economy and health. While the pandemic continues to cause casualties in millions, many countries have gone under lockdown. During this period, people have to stay within walls and become more addicted towards social networks.

Lifestyle, Epstein-Barr virus infection, and other factors could impede nasopharyngeal cancer survivorship: a five-year cross-sectional study in North Eastern India.

Unlabelled: Nasopharyngeal Carcinoma (NPC) is one of the leading cancers in India's north-eastern (NE) region affecting a section of the population each year. A proportion of the NPC cases are observed to recur even after therapy, indicating the involvement of other factors. We aimed to explore the NPC and Epstein-Barr virus (EBV) burden in the NE region and investigate the prognostic factors for the NPC patients' poor survival and recurrence.

Biologia Futura: treatment of wastewater and water using tannin-based coagulants.

Industrialization and urbanization are mainly responsible for environmental pollution generating enormous amount of wastewater which needs to be treated. Wastewaters from various sources are toxic to humans and livestock, as well as posing environmental risks. Various treatment approaches have been used for the elimination of contaminants from water and wastewater.

Host and viral non-coding RNAs in dengue pathogenesis.

Dengue virus (DENV) is a mosquito-borne flavivirus that causes frequent outbreaks in tropical countries. Due to the four different serotypes and ever-mutating RNA genome, it is challenging to develop efficient therapeutics. Early diagnosis is crucial to prevent the severe form of dengue, leading to mortality.

BAX -248 G>A and BCL2 -938 C>A Variant Lowers the Survival in Patients with Nasopharyngeal Carcinoma and Could be Associated with Tissue-Specific Malignancies: A Multi-Method Approach.

Background: The association of BAX -248 G>A and BCL2 -938 C>A with different cancers created conflicts.  We studied the correlation and the effect of these polymorphisms in patients with Nasopharyngeal Carcinoma (NPC).  Methods: PCR-RFLP and Sanger sequencing were used to detect polymorphisms.

Wastewater treatment using plant-derived bioflocculants: green chemistry approach for safe environment.

The rapid expansion of global trade and human activities has resulted in a massive increase in wastewater pollution into the atmosphere. Suspended solids, organic and inorganic particles, dissolved solids, heavy metals, dyes, and other impurities contained in wastewater from various sources are toxic to the atmosphere and pose serious health risks to humans and animals. Coagulation-flocculation technology is commonly used in wastewater treatment to remove cell debris, colloids, and contaminants in a comfortable and effective manner.

Green Formulation of Microbial Biopolyesteric Nanocarriers Toward In Vitro Drug Delivery and Its Characterization.

In the present study, formulation and characterization of microbial biopolyesteric nanocarrier (MBPNc) was reported for in vitro controlled release of the drugs, viz., amoxicillin and levofloxacin. The synthesis of microbial biopolyester, poly(3-hydroxybutyrate-co-3-hydroxyvalerate) nanoparticle was done by a triple emulsion method and loaded with amoxicillin and levofloxacin to improve its curative bioavailability.

Fabrication and characterization of biodegradable PHBV/SiO nanocomposite for thermo-mechanical and antibacterial applications in food packaging.

In the present study, biogenic silica nanoparticles (bSNPs) were synthesized from groundnut shells, and thoroughly characterized to understand its phase, and microstructure properties. The biopolymer was synthesized from yeast and identified as Poly (3-hydroxybutyrate--3-hydroxyvalerate) (PHBV) by GC-MS and NMR analysis. The bSNPs were reinforced to fabricate PHBV/SiO nanocomposites via solution casting technique.

Nanoencapsulation of VIT-MN04 using electrospinning technique for easy gastrointestinal transit.

In this study, probiotic yeast () VIT-MN04 was encapsulated with wheat bran fibre (WBF) and exopolysaccharide (EPS) along with 5% polyvinylpyrrolidone (PVP) using electrospinning technique for easy gastrointestinal transit (GIT). The electrospinning materials viz. WBF (10%), EPS (15%), PVP (5%) and electrospinning parameters viz.

Application of Aloe vera mucilage as bioflocculant for the treatment of textile wastewater: process optimization.

Aloe vera is an important commodity plant which has been traditionally used for the treatment of various diseases. This study investigated the use of extracted bioflocculant from Aloe vera for the treatment of textile wastewater. The bioflocculant was extracted, purified and characterized using GC-MS, FTIR, SEM, AFM, EDX and XRD analysis.

Picking up a Fight: Fine Tuning Mitochondrial Innate Immune Defenses Against RNA Viruses.

As the world faces the challenge of the COVID-19 pandemic, it has become an urgent need of the hour to understand how our immune system sense and respond to RNA viruses that are often life-threatening. While most vaccine strategies for these viruses are developed around a programmed antibody response, relatively less attention is paid to our innate immune defenses that can determine the outcome of a viral infection the production of antiviral cytokines like Type I Interferons. However, it is becoming increasingly evident that the "cytokine storm" induced by aberrant activation of the innate immune response against a viral pathogen may sometimes offer replicative advantage to the virus thus promoting disease pathogenesis.

In Vitro Studies on Therapeutic Potential of Probiotic Yeasts Isolated from Various Sources.

The present study investigates the therapeutic properties of probiotic yeasts viz. Yarrowia lipolytica VIT-MN01, Kluyveromyces lactis VIT-MN02, Lipomyces starkeyi VIT-MN03, Saccharomycopsis fibuligera VIT-MN04 and Brettanomyces custersianus VIT-MN05. The antimutagenic activity of probiotic yeasts against the mutagens viz.

Protein extraction from Saccharomyces cerevisiae at different growth phases.

Saccharomyces cerevisiae is an established model organism with a well characterized genome. However, this model presents a unique problem due to a very resistant cell wall which develops in the late stationary phase resulting in sub-optimal extraction of proteins from such cells using majority of the cell lysis protocols. In this study, several methods from the literature with modifications thereof for lysis of S.

Role of Catalase in Oxidative Stress- and Age-Associated Degenerative Diseases.

Reactive species produced in the cell during normal cellular metabolism can chemically react with cellular biomolecules such as nucleic acids, proteins, and lipids, thereby causing their oxidative modifications leading to alterations in their compositions and potential damage to their cellular activities. Fortunately, cells have evolved several antioxidant defense mechanisms (as metabolites, vitamins, and enzymes) to neutralize or mitigate the harmful effect of reactive species and/or their byproducts. Any perturbation in the balance in the level of antioxidants and the reactive species results in a physiological condition called "oxidative stress.

An overview of cephalosporin antibiotics as emerging contaminants: a serious environmental concern.

Antibiotics have been categorized as emerging pollutants due to their indiscriminate usage, continuous input and persistence in various environmental matrices even at lower concentrations. Cephalosporins are the broad-spectrum antibiotics of β-lactam family. Owing to its enormous production and consumption, it is reported as the second most prescribed antibiotic classes in Europe.

Enhanced degradation of indeno(1,2,3-cd)pyrene using NN4 in presence of iron nanoparticles and produced biosurfactant: a statistical approach.

Seven yeast isolates were screened for the remediation of indeno(1,2,3-cd)pyrene (InP) using biosynthesized iron nanoparticles and produced biosurfactant in growth medium. Four yeast isolates showed positive response to produce biosurfactant which was confirmed by drop collapse test, emulsification index, methylene blue agar plate method, oil displacement test and lipase activity. The yeast strain showing maximum potential for InP degradation and biosurfactant production was identified as NN4.

Potential probiotic characterization and effect of encapsulation of probiotic yeast strains on survival in simulated gastrointestinal tract condition.

The present study is focused on probiotic characterization of four yeasts viz. VIT-SJSN01, VIT-ASN04, VIT-ASN01 and VIT-ASN03 isolated from food samples based on their auto-aggregation, co-aggregation ability and haemolytic activity. All the yeast strains showed good self-adhering and co-adhering potentiality with a value index of greater than 85%.

Fluorescent Small Molecules Are BIG Enough To Sense Biomacromolecule: Synthesis of Aromatic Thioesters and Understanding Their Interactions with ctDNA.

The visible fluorescent chromophoric moiety present in the water-soluble photoactive yellow protein (PYP) of is -hydroxycinnamic acid linked to the cysteine residue (Cys-69) by a thioester bond and it controls the key photoinduced biological processes of the host organism. In the present work, we have synthesized and characterized three structurally different thiophenyl esters [viz., hydroxycinnamic-thiophenyl ester (), -,-dimethylaminocinnamic-thiophenyl ester (), and -phenyl-3-(4-chlorophenyl)-3-(phenylthio)propanethioate ()] in addition to a novel (to the best of our knowledge) stilbene-type olefinic compound, ,,,-tetramethyl-1,2-bis(phenylthio)ethene-1,2-diamine (), under the same reaction condition.