Restriction-free cloning for molecular manipulation and augmented expression of banana bunchy top viral coat protein.

Unlabelled: (BBTV) causing bunchy top disease, is one of the most devastating diseases of banana and plantain. All the six genomic components of isolates from different parts of the world have been well characterised, with most of the studies focusing on replicase gene and coat protein gene. Overexpression of coat protein (CP) in system can contribute significantly in structural as well as immunological studies.