[The development of experimental sample of immune chromatographic test-system for detecting protein of pathogenicity of CagA Helicobacter pylori.].

The immunochromatographic test-system was developed for detecting protein of pathogenicity of CagA Helicobacter pylori in various biological samples (feces, content of dento-gingival recesses) and also in culture of H.pylori. The test-system represents multi-membrane composite on the basis of membranes manufactured by "MDI" (India).

[The comparative evaluation of components used in development of immune chromatographic test-systems].

The article summarizes data of research studies concerning searching optimal conditions of development of highly specific and highly sensitive immune chromatography test-systems designed for detection of agents of infectious diseases. The analysis was implemented concerning choosing of optimal size of nano-particles of colloid gold and concentration of antibodies for production of conjugate. The alternatives are presented concerning the most frequently used combinations of buffer solutions applied for development and analysis of immune chromatography test-systems.

[INTESTINAL FAILURE AND YERSINIA PSEUDOTUBERCULOSIS TRANSLOCATION IN THE DEVELOPMENTOF EXPERIMENTAL GENERALIZED INFECTION].

The Objective: To determine the value of intestinal failure and translocation of bacteria Y. pseudotuberculosis, and normal intestinal microbiota in the initiation and generalization of infection in experimental pseudotuberculosis in conventional white mice, as well as pathological manifestation of it as a response to the adhesion and colonization of the mucosus membrane by pathogenic bacteria Y. pseudotuberculosis.

[COMPARATIVE EXPERIMENTAL EVALUATION OF THE EFFECTIVENESS OF MODERN PROBIOTICS, PREBIOTICS, SYNBIOTICS AND METABIOTICS FOR CORRECTION OF INTESTINAL MICROBIOCENOSIS IN ANIMALS WITH ANTIBIOTIC-ASSOCIATED DYSBIOSIS].

Probiotics for decades were leading in the arsenal of tools for correction of intestinal microbiocenosis. However, the response of representatives of intestinal microbiota to enteral intake of probiotics is variable, and in some individuals may be missing. Despite the fact that scientists still disagree on the necessity of forced settlement of normal microbiota at intestinal dysbiosis, large number of medicinal products, functional foods, dietary supplements, which efficiency is not always straightforward, is available for sale.

[The experimental evaluation with flow cytofluorimetry technique of the level of cellular immunologic memory in persons vaccinated against plague and anthrax].

The article deals with experimental evaluation with flow cytofluorimetry technique of the level of cellular immunologic memory in persons vaccinated with plague and anthrax live dry vaccines. It is established that the introduction of plague and anthrax live dry vaccines into organism of vaccinated persons ignites immunologic rearrangement manifested by reliable increase of level of blood concentration of Th1-lymphocytes (immunologic memory cells) against the background of vaccination. The higher correlation coefficient is detected between leucocytes lysis coefficient and stimulation coefficient according blood concentration level of T-lymphocytes predominantly at the expense of Th1-lymphocytes.

[Identification of genes encoding ligninolytic enzymes in naturally occurring basidiomycete isolates].

The presence of genes encoding lignin peroxidase, laccase, and manganese peroxidase was assessed in more than 20 types of polypore fungi collected in the woods of Kirov oblast; the fungi studied had not been previously characterized with regard to ligninolytic enzyme production. Fifteen isolates of eleven basidiomycete species were shown to contain genes coding for all three ligninolytic enzymes. Genes coding for these enzymes were detected in D.

[Current laboratory methods to evaluate the efficiency of immunization against dangerous and extremely dangerous infections].

By taking into account the leading role of specific cellular immunity in the development of protection against the majority of dangerous and extremely dangerous infections of bacterial, viral, and rickettsial nature, the study of the above immunity should be considered as most important on estimating the body's immunological rearrangement when vaccines against respective infections are administered. The battery of the test used to date for the analysis of specific cellular immunity is mainly restricted to skin tests that have disadvantages. This paper reviews the currently available laboratory methods for in vitro studies of a specific cellular immune response as a criterion for evaluating the efficiency of immunization.

[Survival of probiotic microorganisms in the conditions in vitro imitating the process of human digestion].

Purpose Of The Study: Assessment of survival bifidobacteria and lactobacteria under the conditions in vitro, simulating digestion in human stomach and intestine, and study of survival probiotic and indigenous microorganisms in co-cultivation on solid nutrient medium.

Materials And Methods: Probiotic microorganisms from commercial preparations Bifidobacterin and Lactobacterin, clinical isolates lactobacillus (Lactobacillus acidophilus No 1, L. brevis No 2) were used in experiments.

[The development and testing of a molecular biological test system for DNA detection of anthrax pathogen by real-time polymerase chain reaction assay].

The paper presents the results of the development and testing of a molecular biological test system for DNA detection of anthrax pathogen (Bacillus anthracis) by real-time polymerase chain reaction assay. The test system has shown high sensitivity, specificity, and reproducibility of results of analysis, as exemplified by aqueous suspensions of daily agar cultures of Bacillus anthracis strains, related and heterologous species of microorganisms, and clinical materials of experimental animals. There is evidence for the persistence of the basic characteristics of the test system when stored at 22 +/- 2 degrees C for 12 months.

[Experimental study of sensitivity of probiotic microorganisms to antibacterial drugs].

Purpose Of The Study: Investigation of sensitivity of probiotic microorganisms to antibacterial drugs.

Materials And Methods: Probiotic microorganisms from domestic and foreign commercial preparations were used in experiments. The study of sensitivity of probiotic microorganisms to antibacterial drugs was carried out using solid nutrient medium containing calculated amount of antibacterial drugs.

[Comparative assessment of survival of probiotic microorganisms from commercial preparations under the conditions in vitro].

Purpose Of The Study: Assessment of survival of probiotic microorganisms from commercial preparations under the conditions in vitro simulates digestion in human stomach and intestine.

Materials And Methods: Probiotic microorganisms from domestic and foreign commercial preparations were used in experiments. Study of survival of probiotic microorganisms was carried out on the model in vitro simulates the conditions of digestion in the human body.

[Genotyping of the Bacillus anthracis vaccine strains using the multiloci VNTR-analysis].

The genotyping variety of 5 known anthracis vaccine strains using 18 variable loci of the chromosomal localization taken from a microbe culture collection of 48 Research Institute of Ministry of Defense was revealed in the research. The stability of the VNTR-loci was shown to be inherited from the B. anthracis strains with common origin and an opportunity of their gene-identification application.

[The time course of changes in cell immunological parameters during administration of live dry plague vaccine].

The study of the time course of changes in cell immunological parameters by a magnetic separation technique in human beings during the administration of plague vaccine in relation to the immunological load revealed the higher blood levels of all T lymphocyte subpopulations on day 14 after vaccination. These changes are most typical of a primary vaccinated cohort. The increased frequency of plague vaccine administration and multiple immunizations with live plague, anthrax, and tularemia vaccines produce the time-course of changes in T lymphocyte populations (subpopulations) in response to the regular administration of plague vaccine.

[New immunoglobulin for treatment of anthrax].

Aim: To experimentally assess activity and safety of anti-anthrax intravenous immunoglobulin manufactured on standard technology.

Materials And Methods: Plasma from selected donors vaccinated with combined anthrax vaccine was tested by enzyme immunoassay. Samples of plasma with increased titer of anti-anthrax antibodies were merged in one manufacturing load and fractionated in ethanol at negative temperature according to standard technology.

[Temperate Legionella bacteriophage: discovery and characteristics].

For the first time, temperate Legionella bacteriophage was isolated from organs of guinea pig infected with Philadelphia 1 strain of Legionella pneumophila. Negative colonies of bactriophage from 1.5 to 2.

[Identification of clinical isolates of Burkholderia cepacia and assessment of their pathogenicity in animal experiments].

In experiments on animals study of pathogenicity of 9 clinical strains of Burkholderia cepacia isolated from patients with chronic lung diseases was performed. Preliminary identification of studied strains by means of biochemical and genetic methods allowed to establish their belonging to B. cepacia species.

[Assessment of immunity and allergy after vaccination with dry combined anthrax vaccine].

Study of humoral immune response and allergy in recipients of dry combined anthrax vaccine was performed. Immune response was assessed by antibody titers to protective antigen and by index of preventive properties of blood serum (PPS) of recipients. Relation of index of PPS and antibody titers in blood serum of the donors was established.

[Treatment of late stages of anthracic infection].

Ways for increasing the anthrax treatment efficacy by combined use of antibiotics, toxin neutralizing, immunomodulating, symptomatic and pathogenetic agents are considered. The dynamics of the infection development was studied on rabbits by the microbiological, cytochemical, biochemical and cytomorphologic criteria. The efficacy of monoantibacterial and complex schemes of the etiopathogenetic therapy of the disease at the early and late stages (phases) of the infection generalization was estimated.

[The role of the Yersinia plasmid of calcium-dependency in the formation of the plague immunity].

It was shown that viable variants of the Y. enterocolitica microorganisms containing the plasmid of Ca-dependency (native one or from EV/ I Y. pestis cells) were more immunogenic to plague-infected guinea pigs than isogenic non-plasmid variant.

[Current understanding of the pathogenesis of infectious diseases].

New experimental data in such research fields as molecular biology, biochemistry, genomics and others as well as the changes occurring in the medical-and-epidemiological situation in respect to a number of infectious diseases dictate the necessity to systemize and to revise the appropriate knowledge for the purpose of ensuring a more profound understanding of the processes providing a foundation for the relations within the system of "host--parasite". The article deals with modern aspects of investigating the pathogenesis of infectious diseases, i.e.

[Evaluation of the hydrophobicity of bacterial cells by measuring their adherence to chloroform drops].

A simple method for measuring the hydrophobicity of bacterial cells is proposed, which is based on the spectrophotometric evaluation of cell adherence to chloroform drops in a biphasic water-chloroform mixture.