ETS transcription factor ESE-1/Elf3 is an independent prognostic factor of survival in HRHER2 breast cancer patients.

Purpose: The ETS transcription factor ESE-1 has been shown to be important in HER2 breast cancer and ESE-1 mRNA expression has been shown to associate with prognostic outcomes in the HER2 subtype, as well as in ER, HER2 luminal B patients. However, the clinical significance of ESE-1 protein expression remains unknown. The purpose of the current exploratory study is to evaluate the prognostic value of ESE-1 protein expression in molecular breast cancer subtypes with special emphasis on hormone receptor positive HER2(HR HER2) and the HER2 positive (HER2-only) breast cancer patients.

Mapping of ESE-1 subdomains required to initiate mammary epithelial cell transformation via a cytoplasmic mechanism.

Background: The ETS family transcription factor ESE-1 is often overexpressed in human breast cancer. ESE-1 initiates transformation of MCF-12A cells via a non-transcriptional, cytoplasmic process that is mediated by a unique 40-amino acid serine and aspartic acid rich (SAR) subdomain, whereas, ESE-1's nuclear transcriptional property is required to maintain the transformed phenotype of MCF7, ZR-75-1 and T47D breast cancer cells.

Results: To map the minimal functional nuclear localization (NLS) and nuclear export (NES) signals, we fused in-frame putative NLS and NES motifs between GFP and the SAR domain.