Intratumoral hypoxia as the genesis of genetic instability and clinical prognosis in prostate cancer.

Intratumoral hypoxia is prevalent in many solid tumors and is a marker of poor clinical prognosis in prostate cancer. The presence of hypoxia is associated with increased chromosomal instability, gene amplification, downregulation of DNA damage repair pathways, and altered sensitivity to agents that damage DNA. These genomic changes could also lead to oncogene activation or tumor suppressor gene inactivation during prostate cancer progression.

Cloning of the cDNA, localization, and physiological effects of FGLamide-related allatostatins in the blood-gorging bug, Rhodnius prolixus.

Allatostatins (ASTs) are insect neuropeptides that were first identified as inhibitors of juvenile hormone biosynthesis by the corpora allata. There are three families of ASTs in insects, defined by their C-terminus conserved regions, one of which is FGLamide. Here we determine, for the first time in a hemipteran, the complete 1013 bp cDNA sequence encoding the Rhodnius prolixus FGLa/ASTs (Rhopr-FGLa/ASTs), and confirm the transcript size using northern blot.