Publications by authors named "Daria M Motorina"
At present, there are a variety of different approaches to the targeted regulation of gene expression. However, most approaches are devoted to the activation of gene transcription, and the methods for gene silencing are much fewer in number. In this review, we describe the main systems used for the targeted suppression of gene expression (including RNA interference (RNAi), chimeric transcription factors, chimeric zinc finger proteins, transcription activator-like effectors (TALEs)-based repressors, optogenetic tools, and CRISPR/Cas-based repressors) and their application in eukaryotes-plants and animals.
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- Continuous expression of foreign genes in plants can lead to negative effects, but tissue-specific promoters offer a partial solution.
- Inducible transgene expression systems, primarily driven by chemical agents, allow for more controlled gene expression but have certain limitations.
- Optogenetic tools provide a more advanced method for controlling gene expression with light, offering advantages over traditional chemical methods and potential for practical field applications.