scRNA-seq identifies unique macrophage population in murine model of ozone induced asthma exacerbation.

Ozone (O ) inhalation triggers asthmatic airway hyperresponsiveness (AHR), but the mechanisms by which this occurs are unknown. Previously, we developed a murine model of dust mite, ragweed, and (DRA)-induced allergic lung inflammation followed by O exposure for mechanistic investigation. The present study used single cell RNA-sequencing for unbiased profiling of immune cells within the lungs of mice exposed to DRA, O , or DRA+O , to identify the components of the immune cell niche that contribute to AHR.

Ozone impairs endogenous compensatory responses in allergic asthma.

Asthma is a chronic inflammatory airway disease characterized by acute exacerbations triggered by inhaled allergens, respiratory infections, or air pollution. Ozone (O), a major component of air pollution, can damage the lung epithelium in healthy individuals. Despite this association, little is known about the effects of O and its impact on chronic lung disease.