Publications by authors named "Daria Jacob"
Background: Yeast cells represent an established bioreactor to produce recombinant proteins for subunit vaccine development. In addition, delivery of vaccine antigens directly within heat-inactivated yeast cells is attractive due to the adjuvancy provided by the yeast cell. In this study, Pichia pastoris yeast lysates carrying the nucleoprotein (N) from the measles vaccine virus were evaluated as a novel subunit vaccine platform to deliver the circumsporozoite surface antigen (CS) of Plasmodium.
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- - Yeasts, particularly Pichia pastoris, are being explored as a dual system for producing and delivering malaria vaccines using the circumsporozoite protein (CS) from the Plasmodium berghei parasite, avoiding high purification costs and safety concerns with adjuvants.
- - The N-PbCS fusion protein created in yeast forms multimeric ribonucleoprotein rods, which were shown to elicit a significant immune response in C57Bl/6 mice, leading to reduced infection severity.
- - This strategy not only demonstrates the effectiveness of whole yeast as an intrinsic adjuvant but also opens the door for developing multivalent vaccine formulations that could improve malaria immunization efforts.
The ABCA1 domain responsible for interaction with HIV-1 Nef is conformational and not linear.
Biochem Biophys Res Commun
January 2014
HIV-1 Nef is an accessory protein responsible for inactivation of a number of host cell proteins essential for anti-viral immune responses. In most cases, Nef binds to the target protein and directs it to a degradation pathway. Our previous studies demonstrated that Nef impairs activity of the cellular cholesterol transporter, ABCA1, and that Nef interacts with ABCA1.
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