BRAF Mutation-Responsive miRNA-222-3p Promotes Metastasis of Papillary Thyroid Cancer Cells Snail-Induced EMT.

mutation accounts for 50% of the PTC (papillary thyroid carcinoma) and is closely associated with high-risk clinicopathological characteristics. Increasing evidence implied that dysregulation of miRNA participated in carcinogenesis and progression of cancer. Clinical data showed the significant up-regulation of miR-222-3p in PTC; however, the role of miR-222-3p and possible relationship with mutation remained unclear.

Spontaneous Thyroid Hemorrhage Caused by Langerhans Cell Histiocytosis: A Case Report and Literature Review.

Purpose: Langerhans cell histiocytosis (LCH) is a rare clonal disorder of Langerhans antigen-presenting cells. However, thyroid LCH involvement is relatively rare. We present the first case of spontaneous thyroid hemorrhage due to LCH progression and discuss the clinical features, diagnosis, and treatments of thyroid LCH in a literature review.

Unilateral Multifocality and Bilaterality Could Be Two Different Multifocal Entities in Patients with Papillary Thyroid Microcarcinoma.

Objective: Multifocality within an affected lobe (unilateral multifocality) or two lobes (bilaterality) is commonly denoted as multifocality without differentiation. Recently, there has been molecular evidence indicating that unilateral multifocality and bilaterality could be two different entities. However, few studies concerning the comparison between these two different multifocality entities have been reported.

Finger-pressing: a simple and efficient way to stop chyle leak post neck dissection.

Purpose: Postoperative chyle leak (CL) is a rare but severe complication after neck dissection. Conservative first-line treatments, such as total parenteral nutrition (TPN), octreotide and pressure dressing, can significantly prolong the hospital stay. Efficient and well-tolerated conservative treatment options are still lacking.

Appendiceal mucinous cystadenoma presenting as abdominal internal hernia: a case report.

Appendiceal mucinous neoplasm is a rare form of appendix disorder of all appendectomies. Patients may present with many non-special symptoms which the right low quadrant pain is the mostly reported. We reported a case of 70-year-old man who was admitted to emergency for abdominal pain and progressive aggravation of peritoneal irritation sign.

miR-222 expression is correlated with the ATA risk stratifications in papillary thyroid carcinomas.

Background: miR-222 is one of the most consistently overexpressed miRNAs in papillary thyroid carcinoma (PTC). Previous studies demonstrated that miR-222 overexpression conferred high-risk features in PTC patients, suggesting its value in risk-stratification. However, studies in term of miR-222's utility on stratifying PTCs are lacking.

[Determination of docosahexaenoic acid in milk powder by gas chromatography using acid hydrolysis].

A method to determine docosahexenoic acid (DHA) in milk powder by gas chromatography was established. The milk powder samples were hydrolyzed with hydrochloric acid, extracted to get total fatty acids by Soxhlet extractor, then esterified with potassium hydroxide methanol solution to form methyl esters, and treated with sodium hydrogen sulfate. The optimal experiment conditions were obtained from orthogonal experiment L9(3(3)) which performed with three factors and three levels, and it requires the reaction performed with 1 mol/L potassium hydroxide solution at 25 degrees C for 5 min.

Endoscopic thyroidectomy along with bilateral central neck dissection (ETBC) increases the risk of transient hypoparathyroidism for patients with thyroid carcinoma.

Increasing number of patients with thyroid carcinoma, especially young female patients, prefer to choose endoscopic thyroidectomy with bilateral central neck dissection (ETBC) for perfect cosmetic effects. However, the incidence of hypoparathyroidism after ETBC has not been well studied. Ninety six patients with papillary thyroid carcinoma were enrolled.

[Low temperature freezing followed by dispersive solid phase extraction for the determination of 104 pesticide residues in vegetable oils using ultra-performance liquid chromatography-tandem mass spectrometry].

A method using liquid-liquid extraction (LLE) followed by clean-up steps of centrifugation, freezing and dispersive solid phase extraction (D-SPE) and ultra-performance liquid chromatography (UPLC) coupled with tandem mass spectrometry (MS/MS) has been devel- oped for the determination of trace levels of 104 pesticides in vegetable oils. LLE has been optimized to extract these pesticide residues from oils to obtain the highest recoveries of pesticides and the lowest co-extract fat residue in the final extract. In addition, the centrifugation and freezing steps as well as D-SPE with primary secondary amine (PSA), graphite carbon black (GCB) and C18 were used as the clean-up steps to minimize the co-extract fat.

Visual detection of formaldehyde by highly selective fluorophore labeling via gold(III) complex-mediated three-component coupling reaction.

A novel method for visual detection of formaldehyde with excellent selectivity via a gold(iii) complex-mediated three-component coupling reaction of resin-linked sterically bulky amines and fluorescent alkynes has been developed.

Papillary thyroid microcarcinomas located at the middle part of the middle third of the thyroid gland correlates with the presence of neck metastasis.

Background: Papillary thyroid microcarcinomas (PTMCs), located at upper poles of the thyroid, are associated with lateral neck metastasis (LNM) according to previous reports. Controversy remains regarding the correlation between the location of PTMCs and central neck metastasis (CNM).

Methods: Medical records of 949 patients with PTMCs diagnosed between 2010 and 2013 were reviewed retrospectively.

Contrast-enhanced ultrasound (CEUS) facilitated US in detecting lateral neck lymph node metastasis of thyroid cancer patients: diagnosis value and enhancement patterns of malignant lymph nodes.

Objectives: To examine the value of CEUS as a non-invasive tool in detecting lateral neck metastasis (LNM) and the enhancement patterns of malignant lymph nodes (LN) for thyroid cancer patients.

Methods: Eighty-two consecutive patients, who underwent both preoperative non-enhanced US and CEUS examinations, were retrospectively reviewed. All patients underwent lateral neck dissection (LND).

Influence of up-regulation of Notch ligand DLL4 on biological behaviors of human gastric cancer cells.

Aim: To investigate the potential roles of Delta-like ligand 4 (DLL4) on the biological behavior of gastric cancer cells and its molecular mechanisms.

Methods: A recombinant eukaryotic expression vector containing human DLL4 gene was constructed and transfected into the human gastric cancer cell line SGC7901. Clones with up-regulated DLL4 were selected and amplified.

[Detection of foodborne Salmonella typhimurium and Salmonella enteritidis by diplex real-time PCR using TaqMan probe].

Objective: To establish a method to detect Salmonella typhimurium (ST) and Salmonella enteritidis (SE) simultaneously with a dual real-time PCR assay using double-color fluorescent TaqMan probes.

Methods: The primers and probes were designed based on the conservative domain of STM4599 sequence of ST (GenBank: AERV01000023.1) and the specific sequence of SE ( GenBank: AF370707.

Overexpression of human telomerase reverse transcriptase promotes the motility and invasiveness of HepG2 cells in vitro.

Recent studies have indicated that telomerase activity promotes cancer invasion and metastasis, but the underlying mechanism remains unclear. Several studies have shown that expression of exogenous human telomerase reverse transcriptase (hTERT) can promote motility and invasiveness among telomerase-negative tumor cells, and inhibition of endogenous telomerase activity can reduce invasiveness in tumor cells. However, whether overexpression of hTERT can further enhance the motility and invasiveness of telomerase‑positive tumor cells has yet to be determined.

Genetic analysis of chikungunya viruses imported to mainland China in 2008.

Background: Chikungunya virus (CHIKV) has caused large outbreaks worldwide in recent years, especially on the islands of the Indian Ocean and India. The virus is transmitted by mosquitoes (Aedes aegypti), which are widespread in China, with an especially high population density in southern China. Analyses of full-length viral sequences revealed the acquisition of a single adaptive mutation providing a selective advantage for the transmission of CHIKV by this species.

[Develop a statistics analysis software in population genetics using VBA language].

Objective: To develop a statistics analysis software that can be used in STR population genetics for the purpose of promoting and fastening the basic research of STR population genetics.

Methods: Selecting the Microsoft VBA for Excel, which is simple and easy to use, as the program language and using its macro function to develop a statistics analysis software used in STR population genetics.

Results: The software "Easy STR Genetics" based on VBA language, by which the population genetic analysis of STR data can be made, were developed.

STR data for the AmpFlSTR SGM Plus loci from Shantou (south of China).

Allele frequencies for the ten STRs included in the AmpFlSTR SGM Plus kit were obtained from a sample of 132 unrelated Han individuals born in the region of Shantou (south of China).