Association between HLA alleles and sub-phenotype of childhood steroid-sensitive nephrotic syndrome.

Background: Few studies have addressed the effects of human leukocyte antigen (HLA) alleles on different clinical sub-phenotypes in childhood steroid-sensitive nephrotic syndrome (SSNS), including SSNS without recurrence (SSNSWR) and steroid-dependent nephrotic syndrome/frequently relapse nephrotic syndrome (SDNS/FRNS). In this study, we investigated the relationship between HLA system and children with SSNSWR and SDNS/FRNS and clarified the value of HLA allele detection for precise typing of childhood SSNS.

Methods: A total of 241 Chinese Han individuals with SSNS were genotyped using GenCap-WES Capture Kit, and four-digit resolution HLA alleles were imputed from available Genome Wide Association data.

Clinical and molecular features of 38 children with chronic granulomatous disease in mainland china.

Purpose: Chronic granulomatous disease (CGD) is an inherited disorder, with phagocytes failing to produce antimicrobial superoxide due to deficient NADPH oxidase activity. Mutations in the gene encoding CYBB are responsible for the majority of the CGD cases. To date, there have been no reports on large samples of children with CGD in China.

Age- and gender-specific reference values for urine albumin/creatinine ratio in children of southwest China.

Background: The reference value for the urine albumin/creatinine ratio (ACR) varies between races and has not been previously validated in children. We assessed the ACR reference values and the factors that affect them in a population of healthy Chinese children.

Methods: A total of 1986 healthy children (1078 males, 908 females) aged 6-19 y were enrolled.

[Role of connective tissue growth factor (CTGF) in proliferation and migration of pancreatic cancer cells].

Objective: To explore the expression of connective tissue growth factor (CTGF) in pancreatic cancer and its influence on the proliferation and migration of cancer cells.

Methods: The expression of CTGF in pancreatic cell line PANC-1 cells was analyzed by real-time PCR and in pancreatic carcinoma (50 cases) tissues by immunohistochemistry. The ability of proliferation and migration in vitro of PANC-1 cells was tested by MTT assay, scratch test and Boyden chamber test after the CTGF gene was overexpressed by Ad5-CTGF or silenced with Ad5-siCTGF transfection.

[Clinical analysis of 942 cases of Kawasaki disease].

Objective: The study was designed to investigate the clinical characteristics and the effects of therapeutic proposal on Kawasaki disease (KD).

Methods: Clinical features, diagnosis and treatment for totally 942 patients with KD hospitalized during Jan, 2000 to Dec, 2004 were reviewed. Clinical features of typical and incomplete KD were compared.