The effect of apatinib on pharmacokinetic profile of buspirone both in vivo and in vitro.

Objective: In this study, we aimed to investigate the potential interaction of apatinib and buspirone and underlying mechanism.

Methods: UPLC-MS/MS assay was applied to determine the concentrations of buspirone and its main metabolites (1-PP and 6-OH buspirone) after incubated with liver microsomes. Moreover, the connection of in vitro and in vivo was further determined.