Publications by authors named "Dao Duy Hanh"
Itaconic acid is a promising biochemical building block that can be used in polymer synthesis. Itaconic acid is currently produced in industry by the natural producer fungus Aspergillus terreus using glucose as a main carbon source. Most research for itaconic acid production using lignocellulosic-based carbon sources was carried out by A.
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- Itaconic acid (IA) is a promising bio-based material for the polymer industry, with production typically relying on the cis-pathway from the cadA gene in Aspergillus terreus.
- This study explores the production of IA using engineered strains of Corynebacterium glutamicum expressing two alternative pathways: the cis-pathway (Irg1 from Mus musculus) and the trans-pathway (Adi1 and Tad1 from Ustilago maydis).
- Results indicate that the trans-pathway significantly outperforms the cis-pathway, achieving higher IA production yields (up to 12.25 g/L) when using different carbon sources such as glucose, maltose, and sucrose.
Itaconic acid (IA) is a value-added chemical currently produced by Aspergillus terreus from edible glucose and starch but not from inedible lignocellulosic biomass owing to the high sensitivity to fermentation inhibitors present in the hydrolysate of lignocellulosic biomass. To produce IA from lignocellulosic biomass, a gram-positive bacterium, Corynebacterium glutamicum, with a high tolerance to fermentation inhibitors was metabolically engineered to express a fusion protein composed of cis-aconitate decarboxylase from A. terreus responsible for IA formation from cis-aconitate and a maltose-binding protein (malE) from Escherichia coli.
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