Multicenter observational study of patients who underwent cardiac surgery and were hospitalized in an intensive care unit (BraSIS 2): study protocol and statistical analysis plan.

Background: The perioperative management of patients undergoing cardiac surgery is highly complex and involves numerous factors. There is a strong association between cardiac surgery and perioperative complications. The Brazilian Surgical Identification Study (BraSIS 2) aims to assess the incidence of death and early postoperative complications, identify potential risk factors, and examine both the demographic characteristics of patients and the epidemiology of cardiovascular procedures.

Photochemistry upon Charge Separation in Triphenylamine Derivatives from fs to μs.

Quantum chemical methods and time-resolved laser spectroscopy are employed to elucidate ultrafast charge-separation processes in triphenylamine (TPA) derivatives upon photoexcitation. When changing the ambient solvent from non-electron-accepting to electron-acceptor solvents, such as chloroform, a vastly extended and multifaceted photochemistry of TPA derivatives is observed. Following initial excitation, two concurrent charge-transfer processes are identified.

Cardiovascular disease in women with early-onset preeclampsia: a matched case-control study.

Objective: To compare the risk of cardiovascular disease and the occurrence of cardiovascular events in the mid-long term after delivery, between women with and without a history of early-onset preeclampsia.

Methods: A prospective case-control study has been conducted in Hospital Universitario 12 de Octubre, Madrid. 50 women with early-onset preeclampsia (diagnosed < 34 + 0 weeks) who delivered between 2008 and 2017 and a matched group (by age, parity, pregestational body mass index and date of delivery) of 50 women with uncomplicated pregnancies were recruited.

Performance assessment of disposable carbon-based immunosensors for the detection of SARS-CoV-2 infections.

We designed, developed, and clinically tested two rapid antigen-based immunosensors for SARS-CoV-2 detection, enabling diagnosis and viral load quantification for under USD $2. In a first clinical study, a screen-printed disposable carbon-based (SPC) sensor was assessed on prospectively recruited adult participants classified into three study groups: healthy donors (n = 46); SARS-CoV-2-infected symptomatic patients (n = 58); and co-habitants of patients without prior testing (n = 38). Nasopharyngeal aspirates (NA), oropharyngeal swabs (OS), and saliva (SA) samples were obtained from all participants.

Reproductive Health Counseling and Outcomes Among Women With Congenital Heart Defects: Results From the Congenital Heart Survey to Recognize Outcomes, Needs, and Well-Being, 2016-2019.

Background: Guidelines recommend tailored reproductive health counseling for women with congenital heart defects (CHDs) beginning in adolescence, yet provider adherence to recommendations remains understudied, particularly outside specialized cardiac care settings.

Study Design: We conducted a cross-sectional cohort study among women aged 19 to 38 with CHDs, identified from active population-based birth defects registries in three states. Participants completed surveys from 2016 to 2019, including questions about contraception, pregnancy counseling, concerns, and experiences.

The mChoice App, an mHealth Tool for the Monitoring of Preexposure Prophylaxis Adherence and Sexual Behaviors in Young Men Who Have Sex With Men: Usability Evaluation.

Background: Mobile health (mHealth) apps provide easy and quick access for end users to monitor their health-related activities. Features such as medication reminders help end users adhere to their medication schedules and automatically record these actions, thereby helping manage their overall health. Due to insufficient mHealth tools tailored for HIV preventive care in young men who have sex with men (MSM), our study evaluated the usability of the mChoice app, a tool designed to enhance preexposure prophylaxis (PrEP) adherence and promote sexual health (eg, encouraging the use of condoms and being aware of the partner's HIV status and PrEP use).

The role of religiosity and spirituality in coping with sickle cell disease clinical severity.

Objectives: Religiosity and spirituality often play a role in managing chronic diseases. Sickle cell disease (SCD) is a genetic chronic disease associated with lifelong complications, but there is limited analysis of the potential impact of religiosity and spirituality on SCD. This study aims to analyze the association between constructs of religiosity and spirituality with health outcomes (disease severity, depression, and pain) in an SCD population ( = 275).

Modulation of the Sporulation Dynamics in the Plant-Probiotic Bacillus velezensis 83 via Carbon and Quorum-Sensing Metabolites.

Spore-forming Bacilli, such as the plant-associated Bacillus velezensis strains, are widely used as probiotics, known for their safety and substantial health benefits for both animal and plant species. Through differentiation pathways mediated by quorum-sensing metabolites (QSMs), these bacteria develop multiple isogenic subpopulations with distinct phenotypes and ecological functions, including motile cells, matrix-producing/cannibalistic cells, competent cells, spores, and others. However, the heterogeneity in Bacillus populations is a significant limitation for the development of spore-based probiotics, as nutrients supplied during fermentation are consumed through non-target pathways.

Cardiovascular Outcomes in Hospitalized Patients with COVID-19: Does Age Really Matter?

Background: An advanced age elevates risk for COVID-19-related cardiovascular complications and mortality. This study analyzes cardiovascular comorbidities and outcomes in hospitalized COVID-19 patients across age groups to assess its impact.

Methods: The CARDIO COVID 19-20 registry is a prospective, multicenter cohort study of hospitalized SARS-CoV-2 patients across 44 institutions in 14 Latin American countries.

Molecular and immunological features associated with long-term benefits in metastatic NSCLC patients undergoing immune checkpoint blockade.

Introduction: Immunotherapy is firmly established as a treatment regimen in various solid tumors, driven by its exceptional benefits in a selected group of patients. Despite widespread adoption of immune checkpoint blockade (ICB) across diverse solid tumors, the quest for a clinically informative biomarker for long-term benefit remains unmet.

Methods: A total of 49 patients with metastatic NSCLC treated with ICB were included.

Utilizing Recorded Resuscitations for Neonatal Team Process Improvement.

Newborn resuscitation requires health care professionals to quickly assemble into a high-functioning integrated team. At the authors' academic children's hospital, there are billions of permutations of team composition that could attend a complex newborn delivery at any given time. ResusOne, a resuscitation safety and performance improvement program, uses recorded neonatal resuscitations to identify areas for improvement.

Comprehensive analysis of particulate matter, gaseous pollutants, and microbiological contamination in a clothing store from an international chain in Europe.

In this study, indoor air quality was assessed in a store belonging to one of Portugal's largest commercial retail groups. A variety of methodologies was used, including gravimetric analysis for particulate matter (PM), optical monitors for real-time PM measurements, gas sensors and analysers for CO and other gaseous pollutants, diffusion tubes for VOCs, and passive deposition for microorganisms. The average PM concentration indoors was 16.

AI-assisted protein design to rapidly convert antibody sequences to intrabodies targeting diverse peptides and histone modifications.

Intrabodies are engineered antibodies that function inside living cells, enabling therapeutic, diagnostic, and imaging applications. While powerful, their development has been hindered by challenges associated with their folding, solubility, and stability in the reduced intracellular environment. Here, we present an AI-driven pipeline integrating AlphaFold2, ProteinMPNN, and live-cell screening to optimize antibody framework regions while preserving epitope-binding complementarity-determining regions.

Diabetes and Glucose Management in People on Hemodialysis.

Diabetes is a major cause of end-stage kidney disease (ESKD). Glycemic management is challenging in this population, and A1C, commonly used for monitoring glycemic control, is unreliable. Continuous glucose monitoring indices can be used for glycemic monitoring in people with ESKD.

The Potential of Single-Transcription Factor Gene Expression by RT-qPCR for Subtyping Small Cell Lung Cancer.

Complex RNA-seq signatures involving the transcription factors , , and classify Small Cell Lung Cancer (SCLC) into four subtypes: SCLC-A, SCLC-N, SCLC-P, and SCLC-I (triple negative or inflamed). Preliminary studies suggest that identifying these subtypes can guide targeted therapies and potentially improve outcomes. This study aims to evaluate whether the expression levels of these three key transcription factors can effectively classify SCLC subtypes, comparable to the use of individual antibodies in immunohistochemical (IHC) analysis of formalin-fixed, paraffin-embedded (FFPE) tumor samples.

Expert Clinical Interpretation of Continuous Glucose Monitor Reports From Individuals Without Diabetes.

Background: Clinical interpretation of continuous glucose monitoring (CGM) data for people without diabetes has not been well established. This study aimed to investigate concordance among CGM experts in recommending clinical follow-up for individuals without diabetes, based upon their independent review of CGM data.

Methods: We sent a survey out to expert clinicians ( = 18) and asked them to evaluate 20 potentially challenging Dexcom G6 Pro CGM reports (and hemoglobin A1c [HbA1c] and fasting venous blood glucose levels) from individuals without diabetes.

Invisible girls and boys, their rights to a healthy environment and a family environment in the Establecimiento Penitenciario de Mujeres Socabaya-Arequipa-Peru.

Objectives: To analyze how the rights to a healthy environment and a family environment of invisible children are protected in the Socabaya-Arequipa-Peru Women's Prison.

Material And Method: Data taken from the official documents in the following three prison records were used: the incident log, medical statistical report and the activity control book. The selection criteria were based on the availability, plausibility and official nature of the information in the documents.

Folie à Deux in the Family Environment: A Case of Shared Delusion Between a Mother and Her Son.

Shared psychotic disorder (SPD), also called folie à deux, is an uncommon clinical entity characterized by the transmission of delusions from one psychotic individual to another within the framework of a close relationship, typically isolated from the social environment. Although rare, this phenomenon poses a significant diagnostic and therapeutic challenge, particularly in family settings. This report presents a clinical case of folie à deux between a mother and her child, highlighting the complexities of therapeutic management and providing a succinct review of the literature to adequately frame the clinical approach.

Spatial profiling identifies regionally distinct microenvironments and targetable immunosuppressive mechanisms in pediatric osteosarcoma pulmonary metastases.

Osteosarcoma is the most common malignant bone tumor in young patients and remains a significant clinical challenge, particularly in the context of metastatic disease. Despite extensive documentation of genomic alterations in osteosarcoma, studies detailing the immunosuppressive mechanisms within the metastatic osteosarcoma microenvironment are lacking. Our objective was to characterize the spatial transcriptional landscape of metastatic osteosarcoma to reveal these immunosuppressive mechanisms and identify promising therapeutic targets.

Reversible Redox Controlled DNA Condensation by a Simple Noncanonical Dicationic Diphenylalanine Derivative.

We report the reversible redox-controlled DNA condensation using a simple dicationic diphenylalanine derivative which contains a disulfide unit as linker. Despite the conventional belief that DNA condensing agents require a charge of +3 or higher, this dicationic molecule functions below its critical aggregation concentration, representing a non-canonical DNA condensing agent. The interaction with DNA of the studied compound combines electrostatic effects with hydrophobic/stacking interactions provided with the diphenylalanine moiety.

Comorbidities and Healthcare Utilization Among Young Adults With Congenital Heart Defects by Down Syndrome Status-Congenital Heart Survey to Recognize Outcomes, Needs, and wellbeinG, 2016-2019.

Background: Almost half of individuals born with Down syndrome (DS) have congenital heart defects (CHDs). Yet, little is known about the health and healthcare needs of adults with CHDs and DS. Therefore, we examined comorbidities and healthcare utilization of this population.

Assessing associations between stigma and mental health among pre-exposure prophylaxis users in Guatemala.

Introduction: The mental health of gay and bisexual men (GBM) who use pre-exposure prophylaxis (PrEP) may be negatively impacted by experiences of stigma.

Methods: To assess the relationship between stigma and mental health among GBM who use PrEP in Guatemala, we conducted a survey with 162 PrEP users recruited in a community-based PrEP clinic.

Results: In multiple linear regression analyses, greater internalized (ß=1.

Covered stent repair of a veno-venous cavopulmonary window: a case report.

A 69-year-old man had an abnormal intracardiac course of a pacemaker lead. CT angiography demonstrated a window between the right upper pulmonary vein and the superior caval vein. The window was treated with covered stents in the superior caval vein, which was complicated by a chronic pericardial effusion that was treated with a pericardial window 6 months later.

GLP-1RA Use and Thyroid Cancer Risk.

Importance: The increasing use of glucagon-like peptide-1 receptor agonists (GLP-1RA) demands a better understanding of their association with thyroid cancer.

Objective: To estimate the risk of incident thyroid cancer among adults with type 2 diabetes being treated with GLP-1RA vs other common glucose-lowering medications.

Design, Setting, And Participants: This was a prespecified secondary analysis of a target trial emulation of a comparative effectiveness study using claims data for enrollees in commercial, Medicare Advantage, and Medicare fee-for-service plans across the US.