Yeast One-Hybrid Screening for Regulators of in Purple-Fleshed Sweet Potato ( [L] Lam.).

Background: The transcription regulator is known to be involved in anthocyanin biosynthesis in purple-flesh sweet potato (). However, little is known about the upstream transcription regulators on the promoter of .

Methods: Yeast one-hybrid screening was performed on the storage roots of purple-fleshed sweet potato to identity upstream transcription regulators on the promoter of Luciferase reporter assays and Yeast one-hybrid assays were used to verify these upstream binding proteins interacted with the promoter.

Yeast One-Hybrid Screening to Identify Transcription Factors for in the Purple-Fleshed Sweet Potato ( [L.] Lam.).

IbMYB1 is a transcription factor involved in the biosynthesis of anthocyanin in the purple-fleshed sweet potato. So far, few studies have investigated transcription factors that are upstream of the promoter . In this study, a yeast one-hybrid screening aimed at identifying transcription factors upstream of the promoter was performed in the storage roots of the purple-fleshed sweet potato, and IbPDC, IbERF1, and IbPGP19 were identified as upstream binding proteins for the promoter .

Yeast One-Hybrid Screening for Transcription Factors of IbbHLH2 in Purple-Fleshed Sweet Potato.

The transcription factor IbbHLH2 has been identified as involved in the biosynthesis of anthocyanins in purple-flesh sweet potatoes. However, little is known about the upstream transcription regulators of the promoter of in terms of their involvement in anthocyanin biosynthesis. For this study, the transcription regulators of the promoter of were screened via yeast one-hybrid assays in purple-fleshed sweet potato storage roots.

Genome-wide identification and expression analysis of the cyclic nucleotide-gated ion channel (CNGC) gene family in Saccharum spontaneum.

Background: Cyclic nucleotide-gated ion channels (CNGCs) are nonselective cation channels that are ubiquitous in eukaryotic organisms. As Ca channels, some CNGCs have also proven to be K-permeable and involved in plant development and responses to environmental stimuli. Sugarcane is an important sugar and energy crop worldwide.

Storability and Linear Regression Models of Pericarp Browning and Decay in Fifty Litchi ( Sonn.) Cultivars at Room Temperature Storage.

The primary cause for the limited shelf life of litchi fruit is rapid pericarp browning and decay. This study aims to evaluate the storability of 50 litchi varieties and establish a linear regression model for pericarp browning and decay based on 11 postharvest physical and chemical indices after 9 days of storage at room temperature. The results indicated that the average value of the browning index and decay rate significantly increased to 3.

Characterization and expression profiles of the B-box gene family during plant growth and under low-nitrogen stress in Saccharum.

Background: B-box (BBX) zinc-finger transcription factors play crucial roles in plant growth, development, and abiotic stress responses. Nevertheless, little information is available on sugarcane (Saccharum spp.) BBX genes and their expression profiles.

Genome-wide characterization and expression analysis of the growth-regulating factor family in Saccharum.

Background: Growth regulating factors (GRFs) are transcription factors that regulate diverse biological and physiological processes in plants, including growth, development, and abiotic stress. Although GRF family genes have been studied in a variety of plant species, knowledge about the identification and expression patterns of GRFs in sugarcane (Saccharum spp.) is still lacking.

Identification of Genetic Loci for Sugarcane Leaf Angle at Different Developmental Stages by Genome-Wide Association Study.

Sugarcane ( spp.) is an efficient crop mainly used for sugar and bioethanol production. High yield and high sucrose of sugarcane are always the fundamental demands in sugarcane growth worldwide.

Isolation and characterization of ethylene response factor family genes during development, ethylene regulation and stress treatments in papaya fruit.

Ethylene response factors (ERFs) play important roles in fruit development, ripening, defense responses and stress signaling pathways. After harvest, climacteric fruit such as papaya are subject to a range of problems associated with postharvest handling and storage treatments. There have been few attempts to evaluate the role of ERFs in fruit's responses to environmental stimuli.

Evaluation of new reference genes in papaya for accurate transcript normalization under different experimental conditions.

Real-time reverse transcription PCR (RT-qPCR) is a preferred method for rapid and accurate quantification of gene expression studies. Appropriate application of RT-qPCR requires accurate normalization though the use of reference genes. As no single reference gene is universally suitable for all experiments, thus reference gene(s) validation under different experimental conditions is crucial for RT-qPCR analysis.