[Genoserotyping of Listeria monocytogenes in multiplex PCR].

Listeria monocytogenes infection affects people throughout the all world, most often causing gastrointestinal disturbances, less inflammation of the central nervous system (meningitis, meningoencephalitis, absceses), sepsis, endocarditis and dispersed focal inflammation. Listeria monocytogenes infection in pregnant woman can lead also to premature birth or abortion. Listeriosis is a serious problem because of the high mortality in case of generalized infection, which can reach up to 20%.

[Characteristic of Listeria spp. bacteria isolated from food products].

The frequency of occurrance of Listeria strains in different food products was determined. Biochemical characteristic of the isolated strains was achieved in accordance with procedure included in PNEN ISO 11290 standard, genus was determined byApiListeria (bioMéieux) test. Sensitivity to selected antibacterial medicines was investigated using disck method and Mueller-Hinton 2 Agar medium.

[Drug resistant coagulase-positive staphylococci strains in food].

From the 1572 food samples, examined in Microbiology Department of Frozen Food Industry Research Laboratory in Lódź, 79 (5.0%) coagulase-positive staphylococci strains were isolated. All the strains were sensitive to vancomycin, gentamycin and chloramphenicol.

[Assessment of microbiological quality of minced meat designed for retail sale].

In the years 2000-2001 eighty nine portions (445 samples) of minced meat (beef and pork, beef, pork, veal, turkey) produced by supermarkets and other meat producers from Łódź and around Łódź were examined to check if they meet the standard requirements of PN-97/A-82009 and PN-97/A-82009/A1. The following parameters were determined according to PN-94/A-82055: the total number of microorganisms, most probable number of Escherichia coli, enumeration of Staphylococcus aureus and detection of Salmonella. In addition the presence of Listeria was determined in 25 g of examined meat.

[Occurrence of beta-lactamase types ESBL and AmpC among gram-negative rods isolated from food].

The aim of the study was to isolate extended spectrum beta-laktamases (ESBL) and chromosomal beta-laktamases AmpC producing strains from food products. A total of 739 Gram-negative bacteria were tested with double disc diffusion method using cefotaxim, ceftasidim and amoxycillin with clavulanic acid. One strain producing ESBL and belonging to Stenotrophomonas maltophilia was detected from the ice cream (0.