First Report of Leaf Blight Caused by on in China.

(Makino) T. C. Chen is an annual plant in the Fabaceae.

A Simple and Efficient CRISPR/Cas9 System Using a Ribonucleoprotein Method for .

CRISPR/Cas9 systems were established in some edible fungi based on in vivo expressed Cas9 and guide RNA. Compared with those systems, the in vitro assembled Cas9 and sgRNA ribonucleoprotein complexes (RNPs) have more advantages, but only a few examples were reported, and the editing efficiency is relatively low. In this study, we developed and optimized a CRISPR/Cas9 genome-editing method based on in vitro assembled ribonucleoprotein complexes in the mushroom .