Hsa_ circ_0006867 regulates ox-LDL-induced endothelial injury via the miR-499a-3p/ADAM10 axis.

Circular RNAs (circRNAs) have been reported to participate in the development of various diseases. In this study, we investigated the potential mechanism underlying the role of circRNAs in atherosclerosis. Human umbilical vein endothelial cells (HUVECs) were treated with 100 μg/mL oxidized low-density lipoprotein (ox-LDL) to simulate atherosclerosis.

Effect of Lentivirus-Mediated miR-499a-3p on Human Umbilical Vein Endothelial Cells.

Objective: To explore the possible role of miR-499a-3p in the function of primary human umbilical vein endothelial cells (HUVECs) and the expression of ADAM10 in primary HUVEC.

Method: miR-499a-3p was first transfected into primary HUVECs via lentivirus vector. The viability, proliferation, and migration of stable transfected primary HUVEC were then determined by flow cytometry, CCK8 assays, scratch tests, and Transwell tests.

Progress in the Mechanism and Clinical Application of Cilostazol.

Cilostazol is a unique platelet inhibitor that has been used clinically for more than 20 years. As a phosphodiesterase type III inhibitor, cilostazol is capable of reversible inhibition of platelet aggregation and vasodilation, has antiproliferative effects, and is widely used in the treatment of peripheral arterial disease, cerebrovascular disease, percutaneous coronary intervention, etc. This article briefly reviews the pharmacological mechanisms and clinical application of cilostazol.

Application of Genome-Wide Association Studies in Coronary Artery Disease.

Coronary artery disease (CAD) is a complex disease caused by the combination of environmental and genetic factors. It is one of the leading causes of death and disability in the world. Much research has been focussed on CAD genetic mechanism.

Cyclometalated Ir(iii) complexes containing quinoline-benzimidazole-based N^N ancillary ligands: structural and luminescence modulation by varying the substituent groups or the protonation/deprotonation state of imidazole units.

Cyclometalated Ir(iii) complexes [Ir(dfppy)(qbiH)](PF) (1), [Ir(dfppy)(qbim)](PF)·HO (2), [Ir(dfppy)(qbio)](PF) (3) and [Ir(dfppy)(qbi)] (4) have been designed and prepared, in which the N^N ligands qbiH, qbim and qbio incorporate different substituent groups R on their imidazole units (H atom, CH group and n-CH group, respectively) in order to explore the influence of the substituent groups R and the protonation/deprotonation state of imidazole units in these Ir(iii) complexes on their structures and luminescence behaviors. Crystal structures indicate that an {Ir(dfppy)} unit is coordinated by neutral ligands qbiH in 1, qbim in 2 and qbio in 3, while a qbi anion in 4. These Ir(iii) complexes show clearly different molecular stacking modes.

Heteroleptic Ir(iii) and Pt(ii) complexes based on 2-(2,4-difluorophenyl)-pyridine and bisthienylethene BrLH: the influence of the metal center on structures, luminescence and photochromism.

Heteroleptic complexes [Ir(dfppy)2(BrL)]·3CH3OH () and [Pt(dfppy)(BrL)]·CH3OH () have been prepared based on the same ligands including bisthienylethene BrLH and dfppyH = (2-(2,4-difluorophenyl)-pyridine). Complexes and reveal distinct crystal structures. The BrL(-) anion uses its phenol-imidazole moiety to coordinate with an {Ir(dfppy)2}(+) unit in the former, while with a {Pt(dfppy)}(+) unit in the latter.

Proteomic analysis of mitral valve in Lewis rat with acute rheumatic heart disease.

Rheumatic heart disease (RHD) makes a heavy burden in human lives and economy. The proteomic analysis of acute rheumatic heart disease (ARHD) can provide precious data to study RHD at the early stages, but no one has looked into. So based on our early research we applied the method of continuous GAS stimulation on Lewis rats to duplicate the animal model of ARHD.

Bisthienylethene Th2im and its complex (Th2imH)2[ReCl6]: crystalline-phase photochromism, and photochemical regulation of luminescence and magnetic properties.

Molecular assembly of bisthienylethene Th2im (1) and [ReCl6]2− anions leads to the complex (Th2imH)2[ReCl6] (2), in which a [ReCl6]2(-) anion connects two equivalent Th2imH+ cations through Cl⋯N/C hydrogen bonds. Crystal structures of 1 and 2 indicate that two thiophene groups of each Th2im/Th2imH+ molecule adopt a photoactive antiparallel conformation. Thus, two compounds show crystalline-phase photochromism (CPP), i.

2-(Anthracenyl)-4,5-bis(2,5-dimethyl(3-thienyl))-1-imidazole: regulatable stacking structures, reversible grinding- and heating-induced emission switching, and solid-state photodimerization behavior.

2-(Anthracenyl)-4,5-bis(2,5-dimethyl(3-thienyl))-1-imidazole (anbdtiH) has been synthesized. Its three solid-state structures, anbdtiH·CHCl (), anbdtiH·2CHOH () and anbdtiH·CFCOO·CHOH·HO (), have been constructed by skillfully choosing CHCl or CHOH as the solvent and using or not using CFCOOH, with the aim of modifying the intermolecular hydrogen bonds and/or π···π stacking interactions. The three distinct structures show significantly different solid-state luminescence behaviors, an orange-red emission at 603 nm for , a blue emission at 453 nm for , and a green emission at 533 nm for .

Two bisthienylethene-Ir(III) complexes showing acid/base-induced structural transformation and on-off luminescence switching in solution.

Complexes [Ir(dfppy)2(pbdtiH)](PF6)·2CHCl3 (1-H) and [Ir(dfppy)2(pbdti)] (1) were synthesized by the reaction of bisthienylethene pbdtiH and an [Ir(dfppy)2Cl]2 dimer under neutral and basic conditions, respectively. Thus, the {Ir(dfppy)2}(+) unit is coordinated by pbdtiH in 1-H, and by pbdti(-) in 1, which are confirmed by their crystal structures. The structures of 1-H and 1 could be interconverted in solution, upon alternately adding NEt3 and TFA, thus resulting in reversible luminescence switching between the on-state of 1-H and the off-state of 1 at room temperature.