The earliest cotton fibers and Pan-regional contacts in the Near East.

Fiber technology (cordage and textile) has played a central role in all human societies for thousands of years, and its production, application and exchange have deep roots in prehistory. However, fiber remains have only rarely been observed in prehistoric sites because they tend to decay quickly in normal environmental conditions. To overcome preservation problems of macroscopic remains, we employed microbotanical analysis on soils from anthropogenic sediments in activity areas at Tel Tsaf in the Jordan Valley, Israel (ca.

Long-distance trade in the Middle Chalcolithic of the southern Levant: The case of the olivine beads from Tel Tsaf, Jordan Valley, Israel.

Eight olivine beads found at the Middle Chalcolithic site of Tel Tsaf (ca. 5,200-4,700 cal. BC), Jordan Valley, Israel, underscore a new facet of interregional exchange for this period.

Massively parallel pooled screening reveals genomic determinants of nanoparticle delivery.

To accelerate the translation of cancer nanomedicine, we used an integrated genomic approach to improve our understanding of the cellular processes that govern nanoparticle trafficking. We developed a massively parallel screen that leverages barcoded, pooled cancer cell lines annotated with multiomic data to investigate cell association patterns across a nanoparticle library spanning a range of formulations with clinical potential. We identified both materials properties and cell-intrinsic features that mediate nanoparticle-cell association.

Systematic identification of biomarker-driven drug combinations to overcome resistance.

The ability to understand and predict variable responses to therapeutic agents may improve outcomes in patients with cancer. We hypothesized that the basal gene-transcription state of cancer cell lines, coupled with cell viability profiles of small molecules, might be leveraged to nominate specific mechanisms of intrinsic resistance and to predict drug combinations that overcome resistance. We analyzed 564,424 sensitivity profiles to identify candidate gene-compound pairs, and validated nine such relationships.

Bee products in the prehistoric southern levant: evidence from the lipid organic record.

Beehive products have a rich global history. In the wider Levantine region, bees had a significant role in Egypt and Mesopotamia, and intensive beekeeping was noted in Israel during the Biblical period when apiaries were first identified. This study investigates the origins of this extensive beekeeping through organic residue analysis of pottery from prehistoric sites in the southern Levant.

The function of the south-Levantine Late Chalcolithic and Early Bronze Age basalt vessels bearing circumferential depressions: Insights from use-wear analyses.

One of the most characteristic aspects of the Late Chalcolithic and Early Bronze Age periods in the southern Levant is the appearance of large assemblages of basalt vessels. These vessels, frequently meticulously made, appear sometimes a considerable distance from the raw material sources and are found mainly at habitation sites. While these and their prestigious value have been widely discussed in the past, their function is still obscure.

Exploring exchange and direct procurement strategies for Natufian food processing tools of el-Wad Terrace, Israel.

We present the results of a detailed geochemical provenance study of 54 Natufian (ca. 15,000-11,700 cal. BP) basalt pestles from the site of el-Wad Terrace (EWT), Israel.

A metastasis map of human cancer cell lines.

Most deaths from cancer are explained by metastasis, and yet large-scale metastasis research has been impractical owing to the complexity of in vivo models. Here we introduce an in vivo barcoding strategy that is capable of determining the metastatic potential of human cancer cell lines in mouse xenografts at scale. We validated the robustness, scalability and reproducibility of the method and applied it to 500 cell lines spanning 21 types of solid tumour.

A Middle Pleistocene abrading tool from Tabun Cave, Israel: A search for the roots of abrading technology in human evolution.

During the reanalysis of the finds from Jelinek's and Ronen's excavations at Tabun Cave, Israel, we encountered a cobble bearing traces of mechanical alterations similar to those recorded on grinding tools. However, the artifact derives from the early layers of the Acheulo-Yabrudian complex of the late Lower Paleolithic (ca. 350 ka), a time with no evidence for grinding or abrasion.

7,200 years old constructions and mudbrick technology: The evidence from Tel Tsaf, Jordan Valley, Israel.

The history of mudbrick production and construction in the southern Levant may be dated as far back to the Pre-Pottery Neolithic A. However, at many of the sites where mudbrick remains were noted, their preservation was poor, so investigation of their production and the related construction techniques in antiquity was precluded. The 7,200 year old (cal BP) site of Tel Tsaf, located in the Jordan Valley, is distinguished by outstanding preservation of mudbrick architecture, which enables us to delve into various issues related to mudbrick technology, construction and preservation.

SUMO targeting of a stress-tolerant Ulp1 SUMO protease.

SUMO proteases of the SENP/Ulp family are master regulators of both sumoylation and desumoylation and regulate SUMO homeostasis in eukaryotic cells. SUMO conjugates rapidly increase in response to cellular stress, including nutrient starvation, hypoxia, osmotic stress, DNA damage, heat shock, and other proteotoxic stressors. Nevertheless, little is known about the regulation and targeting of SUMO proteases during stress.

The beginning of metallurgy in the southern Levant: a late 6th millennium CalBC copper awl from Tel Tsaf, Israel.

The beginning of metallurgy in the ancient Near East attracts much attention. The southern Levant, with the rich assemblage of copper artifacts from the Nahal Mishmar cave and the unique gold rings of the Nahal Qanah cave, is regarded as a main center of early metallurgy during the second half of the 5th millennium CalBC. However, a recently discovered copper awl from a Middle Chalcolithic burial at Tel Tsaf, Jordan Valley, Israel, suggests that cast metal technology was introduced to the region as early as the late 6th millennium CalBC.

Earliest floral grave lining from 13,700-11,700-y-old Natufian burials at Raqefet Cave, Mt. Carmel, Israel.

Flowering plants possess mechanisms that stimulate positive emotional and social responses in humans. It is difficult to establish when people started to use flowers in public and ceremonial events because of the scarcity of relevant evidence in the archaeological record. We report on uniquely preserved 13,700-11,700-y-old grave linings made of flowers, suggesting that such use began much earlier than previously thought.