Novel Application of Non-Invasive Methodological Approaches in Biomedical Sciences Towards Better Understanding of Marine Teleost Ocular Health and Disease.

Seafood is an important resource for global nutrition and food security, with both land and marine aquaculture playing pivotal roles. High visual acuity is key for health and survival of farmed, cultured, and wild fish. Cleaner fish technology to control parasite infestation has become important in marine aquaculture and highlights the importance of visual acuity in the efficacy of cleaner fish species.

Increased water temperature contributes to a chondrogenesis response in the eyes of spotted wolffish.

Adult vertebrate cartilage is usually quiescent. Some vertebrates possess ocular scleral skeletons composed of cartilage or bone. The morphological characteristics of the spotted wolffish (Anarhichas minor) scleral skeleton have not been described.

Systematic analysis of ocular features and responses of cultured spotted wolffish (Anarhichas minor).

A better understanding of unique anatomical and functional features of the visual systems of teleost fish could provide key knowledge on how these systems influence the health and survival of these animals in both wild and culture environments. We took a systematic approach to assess some of the visual systems of spotted wolffish (Anarhichas minor), a species of increasing importance in North Atlantic aquaculture initiatives. The lumpfish (Cyclopterus lumpus) was included in these studies in a comparative manner to provide reference.

Inactivated Aeromonas salmonicida impairs adaptive immunity in lumpfish (Cyclopterus lumpus).

Aeromonas salmonicida, a widely distributed aquatic pathogen causing furunculosis in fish, exhibits varied virulence, posing challenges in infectious disease and immunity studies, notably in vaccine efficacy assessment. Lumpfish (Cyclopterus lumpus) has become a valuable model for marine pathogenesis studies. This study evaluated several antigen preparations against A.

Transcriptome profiling of lumpfish (Cyclopterus lumpus) head kidney to Renibacterium salmoninarum at early and chronic infection stages.

Renibacterium salmoninarum causes Bacterial Kidney Disease (BKD) in several fish species. Atlantic lumpfish, a cleaner fish, is susceptible to R. salmoninarum.

Fine-scale environmentally associated spatial structure of lumpfish () across the Northwest Atlantic.

Lumpfish, , have historically been harvested throughout Atlantic Canada and are increasingly in demand as a solution to controlling sea lice in Atlantic salmon farms-a process which involves both the domestication and the transfer of lumpfish between geographic regions. At present, little is known regarding population structure and diversity of wild lumpfish in Atlantic Canada, limiting attempts to assess the potential impacts of escaped lumpfish individuals from salmon pens on currently at-risk wild populations. Here, we characterize the spatial population structure and genomic-environmental associations of wild populations of lumpfish throughout the Northwest Atlantic using both 70K SNP array data and whole-genome re-sequencing data (WGS).

Role of riboflavin biosynthesis gene duplication and transporter in virulence in marine teleost fish.

Active flavins derived from riboflavin (vitamin B) are essential for life. Bacteria biosynthesize riboflavin or scavenge it through uptake systems, and both mechanisms may be present. Because of riboflavin's critical importance, the redundancy of riboflavin biosynthetic pathway (RBP) genes might be present.

Multi-Organ Transcriptome Response of Lumpfish () to Subspecies Systemic Infection.

Lumpfish is utilized as a cleaner fish to biocontrol sealice infestations in Atlantic salmon farms. , a Gram-negative facultative intracellular pathogen, is the causative agent of furunculosis in several fish species, including lumpfish. In this study, lumpfish were intraperitoneally injected with different doses of to calculate the LD.

CD45 in ocular tissues during larval and juvenile stages and early stages of V. anguillarum infection in young lumpfish (Cyclopterus lumpus).

Immune responses to infectious diseases impacting lumpfish (Cyclopterus lumpus) eye tissue are only starting to be studied at a molecular and histopathological level. In this study, we extend our understanding of lumpfish sensory organ anatomy, of components of the lumpfish nasal and ocular immune system and the nature of the intraocular response to Vibrio anguillarum infection. We have evaluated the expression of cluster of differentiation (CD) 45 protein, a tyrosine phosphatase, in larval and juvenile lumpfish tissues in order to spatially survey ocular and related head structures that may participate in early stages of intraocular immune responses.

Characterization of miRNAs in Embryonic, Larval, and Adult Lumpfish Provides a Reference miRNAome for .

MicroRNAs (miRNAs) are endogenous small RNA molecules involved in the post-transcriptional regulation of protein expression by binding to the mRNA of target genes. They are key regulators in teleost development, maintenance of tissue-specific functions, and immune responses. Lumpfish () is becoming an emergent aquaculture species as it has been utilized as a cleaner fish to biocontrol sea lice (e.

Lumpfish () Is Susceptible to Infection and Induces Cell-Mediated Immunity in the Chronic Stage.

is a Gram-positive, intracellular pathogen that causes Bacterial Kidney Disease (BKD) in several fish species in freshwater and seawater. Lumpfish () is utilized as a cleaner fish to biocontrol sea lice infestation in Atlantic salmon () farms. Atlantic salmon is susceptible to , and it can transfer the infection to other fish species.

Reference genome of lumpfish Cyclopterus lumpus Linnaeus provides evidence of male heterogametic sex determination through the AMH pathway.

Teleosts exhibit extensive diversity of sex determination (SD) systems and mechanisms, providing the opportunity to study the evolution of SD and sex chromosomes. Here we sequenced the genome of the common lumpfish (Cyclopterus lumpus Linnaeus), a species of increasing importance to aquaculture, and identified the SD region and master SD locus using a 70 K single nucleotide polymorphism array and tissue-specific expression data. The chromosome-level assembly identified 25 diploid chromosomes with a total size of 572.

Oral Immunization of Larvae and Juvenile of Lumpfish () against Does Not Influence Systemic Immunity.

, a marine bacterial pathogen that causes vibriosis, is a recurrent pathogen of lumpfish Lumpfish is utilized as a cleaner fish in the Atlantic salmon () aquaculture in the North Atlantic region because of its ability to visualize and prey on the ectoparasite sea lice () on the skin of Atlantic salmon, and its performance in cold environments. Lumpfish immunity is critical for optimal performance and sea lice removal. Oral vaccine delivery at a young age is the desired method for fish immunization because is easy to use, reduces fish stress during immunization, and can be applied on a large scale while the fish are at a young age.

A Novel Marine Pathogen Isolated from Wild Cunners (): Comparative Genomics and Transcriptome Profiling of sp. Strain J380.

Cunner () is a cleaner fish being considered for utilized in the North Atlantic salmon () aquaculture industry to biocontrol sea lice infestations. However, bacterial diseases due to natural infections in wild cunners have yet to be described. This study reports the isolation of sp.

CD10 Cells and IgM in Pathogen Response in Lumpfish () Eye Tissues.

Lumpfish (), a North Atlantic "cleaner" fish, is utilized to biocontrol salmon louse () in Atlantic salmon () farms. Lumpfish require excellent vision to scan for and eat louse on salmon skin. The lumpfish eye immune response to infectious diseases has not been explored.

Comparative Genomics Analysis of Isolated from Lumpfish () in Newfoundland Reveal Novel Chromosomal Organizations.

is a Gram-negative marine pathogen causative agent of vibriosis in a wide range of hosts, including invertebrates and teleosts. Lumpfish (), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to control sea lice () infestations in the Atlantic salmon () aquaculture industry. is one of the most frequent bacterial pathogens affecting lumpfish.

Ocular tissue changes associated with anterior segment opacity in lumpfish (Cyclopterus lumpus L) eye.

Lumpfish use their vision to hunt prey or, in the case of aquaculture, to see and eat pelleted diets. A common anterior ocular opacity abnormality designated in the literature as "cataract" described in both farmed and wild lumpfish has not yet been characterized in detail at the pathobiological level. We describe here lens tissue changes associated with cataract in cultured and domesticated lumpfish.

Vibrogen-2 vaccine trial in lumpfish (Cyclopterus lumpus) against Vibrio anguillarum.

Lumpfish (Cyclopterus lumpus), a native fish of the North Atlantic Ocean, is utilized as cleaner fish to biocontrol sea lice infestations in Atlantic salmon aquaculture. However, bacterial infections are affecting cleaner fish performance. Vibrio anguillarum, the aetiological agent of vibriosis, is one of the most frequent bacterial infections in lumpfish, and effective vaccine programmes against this pathogen have been identified as a high priority for lumpfish.

Novel characteristics of the cultured Lumpfish Cyclopterus lumpus eye during post-hatch larval and juvenile developmental stages.

We systematically analysed the characteristics of the Cyclopterus lumpus eye and retina during cultured post-hatch developmental stages using gross observations, histology, immunohistochemistry, microscopy, fundus imaging and spectral domain optical coherence tomography retinal imaging. Post-hatch developing cultured C. lumpus eye and retinal tissues share a number of features typically conserved in other teleost fish.