Publications by authors named "Danivanh Ben-Guigui"
Imaging mass cytometry (IMC) is a metal mass spectrometry-based method allowing highly multiplex immunophenotyping of cells within tissue samples. However, some limitations of IMC are its 1-µm resolution and its time and costs of analysis limiting respectively the detailed histopathological analysis of IMC-produced images and its application to small selected tissue regions of interest (ROI) of one to few square millimeters. Coupling on a single-tissue section, IMC and histopathological analyses could permit a better selection of the ROI for IMC analysis as well as co-analysis of immunophenotyping and histopathological data until the single-cell level.
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- Persistent inflammation can lead to the formation of tertiary lymphoid structures (TLS) that resemble secondary lymphoid organs (SLO) like lymph nodes, with variations in TLS composition linked to different diseases.
- Researchers conducted an analysis of colorectal and gastric tissues from patients with various inflammatory diseases and cancers, utilizing imaging mass cytometry (IMC) to compare TLS and SLO based on 39 specific markers.
- The study found that while TLS organization varies among patients, it follows a maturation spectrum categorized into three stages: lymphoid-aggregates, non-GC TLS, and GC-like TLS, revealing insights into the architectural and functional differences that can have implications for diagnostic and prognostic assessments in diseases.