Publications by authors named "Danilyn Amerna"
Background: Radiation therapy is the standard of care for central nervous system tumours. Despite the success of radiation therapy in reducing tumour mass, irradiation (IR)-induced vasculopathies and neuroinflammation contribute to late-delayed complications, neurodegeneration, and premature ageing in long-term cancer survivors. Mesenchymal stromal cells (MSCs) are adult stem cells that facilitate tissue integrity, homeostasis, and repair.
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- - The study developed a novel toolkit using mutant methionyl-tRNA synthetase (MetRS) to isolate proteins secreted by mesenchymal stromal cells (MSCs) in mixed cultures, which is important for understanding tissue repair.
- - By integrating MetRS into various cell types and using click chemistry, researchers were able to successfully profile the secretome of MSCs, highlighting changes when co-cultured with different conditions, like lipopolysaccharide-treated cells.
- - This new methodology allows for enhanced investigation of MSC responses to diseases and could lead to revelations about tissue regeneration and repair mechanisms in a range of cell types derived from induced pluripotent stem cells (iPSCs).
Article Synopsis
- Intracytoplasmic inclusions of alpha-synuclein (αsyn) are key features in neurological disorders like Parkinson's disease, where reducing αsyn levels is an important goal for treatment.
- * Honokiol (HKL), a compound from magnolia bark with neuroprotective effects, was tested to see if it can lower αsyn levels in various experimental models, including human cells and mouse neurons.
- * The study found that HKL effectively reduces αsyn protein and gene expression through post-transcriptional mechanisms, suggesting it could be a valuable strategy to slow the progression of Parkinson's disease and related conditions.
Mesenchymal stromal cells (MSCs) have a dynamic secretome that plays a critical role in tissue repair and regeneration. However, studying the MSC secretome in mixed-culture disease models remains challenging. This study aimed to develop a mutant methionyl-tRNA synthetase-based toolkit (MetRS ) to selectively profile secreted proteins from MSCs in mixed-culture systems and demonstrate its potential for investigating MSC responses to pathological stimulation.
View Article and Find Full Text PDFApproximately half of Alzheimer's disease (AD) brains have concomitant Lewy pathology at autopsy, suggesting that α-synuclein (α-SYN) aggregation is a regulated event in the pathogenesis of AD. Genome-wide association studies revealed that the ε4 allele of the apolipoprotein E (APOE4) gene, the strongest genetic risk factor for AD, is also the most replicated genetic risk factor for Lewy body dementia (LBD), signifying an important role of APOE4 in both amyloid-β (Aβ) and α-SYN pathogenesis. How APOE4 modulates α-SYN aggregation in AD is unclear.
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