[Inhibition of bacterial luminescence by cytochrome P-450 substrates].

The mechanisms of luminescence quenching by various drugs, e.g. dimethylaniline, ethylmorphine, hexobarbital and aminopyrine, which are effective inhibitors of luminescence both in intact cells and in bacterial luciferase, were studied.

[Inhibitory analysis of the luminescent electron transport chain of Photobacterium fischeri].

The quenching of luminescence of bacterial luciferase from Photobacterium fischeri by non-specific electron acceptors and inhibitors of dehydrogenases was studied. The inhibition of the luminescent reaction obeys the non-competitive mechanism with NADH, FMN and aliphatic aldehyde. The inhibitors compete with cytochrome c for NADH -- cytochrome c oxido-reductase.

[Fauna and biology of the mosquitoes (Culicidae) of the Chara valley (Chita Province)].

The specific composition and the nature of domination of bloodsucking mosquitoes from the Charskaya hollow were studied. The abundance dynamics of attacking females was registered by means of Berezantzev's bell. Data were obtained on the hatching sites of mosquitoes and peculiarities of seasonal adaptation of some species in connection with the availability of various landscapes with different microclimatic conditions.

[Isolation and purification of bacterial luciferase from Photobacterium fischeri for analytical purposes].

A procedure resulting in a highly purified preparation of bacterial luciferase with a high specific activity towards FMNH2 and NADH was developed. Using SDS-electrophoresis in polyacrylamide gel, it was shown that the enzyme has a subunit composition and that its monomers do not contain the luciferase activity. The use of the obtained preparation for determining the content of NADH and FMN by the bioluminescent method allowed to increase its sensitivity up to 10(-18) and 10(-17) moles, respectively.

[Effect of furan compounds on lipid peroxidation in rat liver].

Effect of some furan compounds--monofurfurilidene acetone (MFA), difurfurilidene acetone (DIFA), furyl alcohol (Fal) and furfurol (Fl)--on peroxidation of unsaturated fatty acids in lipids was studied in subcellular organelles of rat liver cells in vitro. By the rate of inhibition of lipid peroxidation the substances studied were arranged as follows: DIFA--100%, Fal--65%, Fl--27% and MFA--1-2%. The furan derivatives at the concentration 10(-2) M effectively inhibited the lipid peroxidation in subcellular organelles, especially in mitochondrial and microsomal fractions.

[Cytochromes of the luminescent bacterium, Photobacterium fischeri, their solubilization and relationship to luminescence].

The hemoprotein composition of the luminescent bacterium Photobacterium fischeri was studied, in particular, the distribution of cytochromes among the bacterial fractions, viz. cell-free extract, supernatant, "particles", protein preparation. The hemochromogenic analysis has shown that the principal hemoproteins of Photobacterium fischeri are cytochromes, with hemes of the b and c type.

[Electron transport systems of Photobacterium fischeri].

The composition of cytochromes was studied in Photobacterium fischeri 6 at different growth phases and under various conditions of cultivation. The electron transport chains of the bacterium are characterized by the presence of cytochromes b and c types. The terminal oxidases are cytochromes o, a2+a1 and P-450.

[Effect of linoleic acid and products of its free radical oxidation on succinate dehydrogenase].

Effects of linoleic acid and its oxidation products (with 75--80% content of hydroperoxide) on isolated succinate dehydrogenase were studied by means of spectrophotometry and ESR-spectroscopy. It was shown that unlike the unoxidized acid, low concentrations of its oxidation products increased catalytic activity, while its high concentrations of its oxidation products increased catalytic activity, while its high concentrations brought about a decrease of SH-group content and the amplitude of ESR signal of reduced nonheme iron in the enzyme.

[Free radical oxidation of biological membrane lipids. VIII. Effect of linoleic acid and its oxidation products on the functional parameters of mitochondria].

Effects of linoleic acid and its oxidation products (with 75--80% content of hydroperoxide) on rat liver mitochondria were studied by polarographic and spectrophotometric methods. Both agents according to their concentration are able to uncouple oxidation and phosphorylation and to inhibit the electron transport in the initial region of the respiration chain as well. Unlike the unoxidized linoleic acid, its oxidation products increase the activity of some enzymatic systems of mitochondria, and at high concentrations they produce a more pronounced effect on the electron transport.

[New species of mosquito, Aedes (Stegomyia) sibiricus sp. n. (Culicidae)].

The description of female, male and 4th stage larva of a new species of mosquito, Aedes (Stegomyia) sibiricus, from Siberia and Far East of the USSR is given. This species belonging to the albopictus subgroup of the scutellaris group of species was formerly confused with A. (S.