[Effect of maladaptive sets on the clinical picture and prediction of chronic headache of tension].

Forty women with chronic headache of tension (CHAT) and 20 women of comparison group with episodic headache of tension (EHAT) were studied. The clinical study included neurological examination with assessment of headache characteristics; psychometric examination - measurement of maladaptive psychological sets with the Pain Sets and Perceptions Inventory (PBPI) and assessment of pain catastrophization using a special scale. Higher scores of maladaptive sets on "mystery" and "stability" PBPI subscales (p<0,05) and on the Pain Catastrophization Scale (p<0,05) were seen in the CHAT group compared to the EHAT.

[Pharmacological PI 3-kinase inhibitors induce radioprotective effect in Drosophila melanogaster imago].

Phosphatidylinositol 3-kinase (PI3K) signaling cascade is involved in many cell processes that are required for radioresistance, including proliferation, survival, growth, metabolism, and death. At the whole organism level PI3K signaling cascade is involved in lifespan control. In this study the contribution of PI3-signaling cascade to radioresistance of Drosophila melanogaster imago was investigated.

Proliferation, migration and differentiation of ependymal region neural progenitor cells in the brainstem after hypoglossal nerve avulsion.

Purpose: Cells in the ependymal region in the adult central nervous system (CNS) have been found to possess neural progenitor cell (NPC) like features including capacity for generating new neurons and glia in response to injury and inflammatory disease. Whether these cells are activated after a peripheral nerve injury has not previously been extensively evaluated.

Methods: We investigate the possible activation and effect of NPCs in the ependymal region in the immediate vicinity to the hypoglossal nucleus in the brainstem using two models of injuries, hypoglossal nerve transection and nerve avulsion after which the proliferation, migration and differentiation of ependymal regional NPCs were evaluated.

[Laboratory evaluation of TGFbeta1, IL-10, VEGF levels in vivo and in vitro in patients with solid tumors].

Tumor cells can acquire the mechanisms of immune response evasion. One of these mechanisms is synthesis and secretion in the microenvironment of immunosuppressive factors able to block immune cells maturation and function. We investigated plasma levels of TGFbeta1 and IL0 in 10 healthy volunteers and 114 patients with solid tumors (breast cancer--24, gastrointestinal tumors--27, renal cell carcinoma--15, lung cancer--9, ovarian cancer--13, cutaneous melanoma--18, primary multiple tumors--2, prostate cancer--6).

[Immunochemical assay of MIC A production by tumor cells in vitro and in vivo as a component of antitumor vaccine development].

Due to immunological monitoring, most of aggressive tumor cells are selected capable of producing soluble factors of immunosuppression in their microenvironment. A ligand for NK- and T-cells receptor (MICA/B) activation is one of them. We investigated MICA concentrations in serum of 10 healthy volunteers and 114 patients with solid tumors (breast cancer - 24, gastrointestinal tumors - 27, renal cell carcinoma - 15, lung cancer - 9, ovarian carcinoma - 13, cutaneous melanoma - 18, primary multiple tumors - 2, prostate cancer - 6).

Dipeptidyl peptidase 2 apoptosis assay determines the B-cell activation stage and predicts prognosis in chronic lymphocytic leukemia.

Objective: Dipeptidyl peptidase 2 (DPP2/DPP7) is a regulator of quiescence as inhibition of DPP2 results in apoptosis of resting, but not activated lymphocytes. The purpose of the present study was to investigate the prognostic value of DPP2 inhibition and the role of DPP2 in cell cycle in chronic lymphocytic leukemia (CLL).

Materials And Methods: We screened 152 peripheral blood samples from patients with CLL in an apoptosis assay with AX8819, a DPP2-specific inhibitor.

[Morpho-functional characteristic of rat fibroblasts after culture and allotransplantation into the dermis].

In model experiments performed on Wistar rats, morpho-functional characteristics of fibroblasts (obtained from the skin of rat pups 2-4 days after their birth) were studied and their viability was estimated shortly after their allotransplantation into the recipient dermis. The results of the research have shown that the suspension of cells received for transplantation after a long-term culture in synthetic media was represented, mainly, by a population of mature fibroblasts. 3 days after transplantation fibroblasts remained viable and preserved their morphological characteristics.

Managing a pregnant patient with paroxysmal nocturnal hemoglobinuria in the era of eculizumab.

Paroxysmal nocturnal hemoglobinuria (PNH) is a rare clonal stem cell disorder, which affects women of child-bearing age. PNH is associated with thrombotic complications, which are the main causes of morbidity and mortality. Management of a pregnant woman with PNH remains a challenge due to high incidence of thrombotic complications and the difficulty of differentiating a PNH crisis from the complications of pregnancy.

[HLA-E molecule induction on the surface of tumor cells protects them from cytotoxic lymphocytes].

Modern immunotherapy has developed powerful tools for mounting antitumor response which nevertheless have had only limited success in clinic. Tumor cells use different mechanisms to escape from immune system. Thus, one of the reasons of unsuccessful immunotherapy might be induction of tolerance of tumor-specific cytotoxic lymphocytes by tumor cells.

[The electrode for selective nociceptive stimulation: use experience and prospect for application in nociception studies in a human].

Electrode for selective nociception specific stimulation was described by H. Kaube et al. in 2000.

Morphofunctional changes in mitochondria during stress.

Electron microscopy revealed morphofunctional changes in mitochondria of rats under stress conditions. Reparative processes and pathomorphological changes were monitored. Heterogeneous ultrastructural changes in mitochondria were revealed.

Ultrastructural and antigenic properties of neural stem cells and their progeny in adult rat subventricular zone.

Neural stem cells (NSCs) in the subventricular zone (SVZ) continuously generate olfactory bulb interneurons in the adult rodent brain. Based on their ultrastructural and antigenic properties, NSCs, transient amplifying precursor cells, and neuroblasts (B, C, and A cells, respectively) have been distinguished in mouse SVZ. Here, we aimed to identify these cell types in rat SVZ ultrastructurally and at the light microscopy level, and to determine the antigenic properties of each cell type using gold and fluorescence immunolabeling.

[An immunocytochemical investigation of antigen expression by skin melanoma cells cultured for vaccine production].

There is a great variety of histological patterns of skin melanoma and, in particular, that of its metastatic patterns. Malignant melanocytes are capable of influencing tumor-associated antigen expression. As of now, several varieties of melanoma-associated antigens (MAA) have been identified: MART1/melan A, tyrosinase, MITF, gp100, members of MAGE family, S100, CD63 and CD146.

Pivotal advance: HMGB1 expression in active lesions of human and experimental multiple sclerosis.

Multiple sclerosis (MS) is a chronic, inflammatory, demyelinating disease of the CNS, most frequently starting with a series of bouts, each followed by complete remission and then a secondary, progressive phase during which the neurological deficit increases steadily. The underlying molecular mechanisms responsible for disease progression are still unclear. Herein, we demonstrate that high mobility group box chromosomal protein 1 (HMGB1), a DNA-binding protein with proinflammatory properties, is evident in active lesions of MS and experimental autoimmune encephalomyelitis (EAE) and that HMGB1 levels correlate with active inflammation.