Study of the upper pole after subtotal splenectomy in rats.

Purpose: To evaluate macro/microscopic viability of the upper pole (UP) in rats after 80 days of subtotal splenectomy preserving the upper pole (SSPUP).

Methods: Twenty-five male Wistar rats were submitted to SSPUP. After 80 days, the rats were euthanized, and the remaining UP was evaluated macroscopically regarding appearance, color, consistency, length, width, thickness, and presence of fibrosis/necrosis; and microscopically regarding presence of red and white pulp, fibrosis/necrosis.

The influence of splenic tissue on the survival and TCD4 and TCD8 lymphocyte rates in rats subjected to fecal peritonitis induction.

Purpose: Comparing survival rates of rats subjected to spleen procedures after fecal peritonitis induction. Assessing changes in TCD4 and CD8 lymphocyte rates before and after the procedures. Correlating animal survival with CD4 and CD8 lymphocytes.

Macro and microscopic comparison of the upper pole of the spleen with the lower pole in partial splenectomy.

Purpose: To evaluate the viability of the upper (UP) and lower pole (LP) of the spleen from a macro and microscopic point of view, after subtotal splenectomy with preservation (SSP) of the UP and the LP.

Methods: Seventeen male Wistar rats, two months old, were submitted to SSPUP and SSPLP and 5 to simulated operation (SG). After 80 days, the rats were euthanized, and the remaining LP and UP and intact spleens were evaluated macroscopically and microscopically.

Maximum inspiratory and expiratory pressures in the pre and postoperative periods of patients with incisional abdominal hernia corrected by Lázaro da Silva technique.

Objective: To verify the effect of longitudinal abdominal incisional herniorrhaphy on respiratory muscle pressure.

Method: The technique of incisional herniorrhaphy used was proposed by Lázaro da Silva. To measure the pressure, we used a water manometer in 20 patients, median age 48.

Effects of inferior splenic lobe pole fixation and gastrosplenic peritoneal membrane section on the vitality of the remanent of subtotal splenectomy in rats.

Purpose: To investigate the vitality of the spleen lower pole after subtotal splenectomy with suture to the stomach and after posterior peritoneal gastro-splenic membrane section, using macro and microscopic evaluations.

Methods: Sixty Wistar rats were used in this study and were randomly distributed in the three groups: Group 1: (n=20), subtotal splenectomy with lower pole preservation, Group 2: (n=20) subtotal splenectomy with lower pole preservation and suture to the stomach, Group 3: subtotal splenectomy with lower pole preservation and posterior peritoneal gastrosplenic ligament section. The animals were sacrificed 45 days after the surgery and the spleen lower poles were removed for macroscopic and microscopic examination.

Effect of total splenectomy in the lipid profile in mice.

Purpose: To analyze total splenectomy effect on the lipid profile - total cholesterol, low-density lipoprotein cholesterol (LDL), high-density lipoprotein cholesterol (HDL), very-low-density lipoprotein cholesterol (VLDL) and triglycerides levels, in Balb/c mice.

Methods: Thirty Balb/c male mice, one (1) month old and average weight 26.2g ± 4.

Micronucleus test in peripheral blood of rats treated with hyperbaric oxygen after subtotal splenectomy preserving the lower pole.

Purpose: To assess the mutagenic potential of the oxygen inhalation therapy (HBO), by means of the micronucleus test, performed in peripheral blood of rats that underwent subtotal splenectomy with lower pole preservation (ESTPI), after HBO sessions or simulations.

Methods: Eighteen male Wistar rats, were distributed into three groups of six animals: group 1 - submitted to ESTPI and HBO sessions; group 2 - submitted to ESTPI and HBO simulations; group 3 - underwent cyclophosphamide administration. In groups 1 and 2, blood samples from the animals' tails were collected before surgery (T0) and immediately after the 13th HBO session or simulation (T1).

Effects of human chorionic gonadotropin on the normal testicular tissue of rats.

Purpose: To investigate the effects of human chorionic gonadotropin (hCG) on the testicular tissue of young male rats.

Methods: Male Wistar rats were assigned to groups (10 rats/group).Control Group received subcutaneous saline solution; Group 1 received hCG 50 UI/Kg/dose; and Group 2 received hCG 100 UI/Kg/dose, daily for 15 days.

Epidural anesthesia with ropivacaine with or without clonidine and postoperative pain in hemorrhoidectomies.

Purpose: To determine the safety, pain intensity correlated with age and body mass index (BMI), epidural anesthesia with ropivacaine and clonidine in hemorrhoidectomy.

Methods: Eighty patients, both genders, 20-70 years old, ASA I or II, for hemorrhoidectomy were randomly divided into two groups: Control (n=38), epidural anesthesia with 14 mL of ropivacaine 0.75 % plus 0.

Results of the simultaneous bilateral inguinal hernia repair by the Lichtenstein technique.

Objective: To analyze the results of bilateral inguinal hernia repairs by the Lichtenstein technique.

Methods: We studied the charts of 59 patients who underwent elective simultaneous bilateral inguinal hernia repair between 2003 and 2007. We analyzed: gender, age, weight, operative time, length of hospital stay, Nyhus classification, complications in the immediate and late postoperative periods, and recurrence.

Immunoexpression of proliferating cell nuclear antigen (PCNA) in spleen of splenectomized rats with preservation of inferior pole, submitted to hyperbaric oxygenation.

Purpose: To analyze PCNA immunoexpression on the inferior pole of the spleen of splenectomized rats submitted to hyperbaric oxygenation (HBO).

Methods: Were analyzed fragments of the inferior pole of the spleen of 20 male Wistar rats submitted to splenectomy with preservation of the inferior pole. The rats were divided in two groups: group A (n=10) without HBO and group B (n=10) submitted to HBO at 2, 5 atmospheres per 120 minutes, twice a day for three days and once a day for seven days.

Decompressive fasciotomy in testicular torsion of the spermatic cord in rats.

Purpose: To evaluate the effect of decompression fasciotomy on testes undergoing torsion of the spermatic cord.

Methods: Spermatic cord torsion was induced in 40 Wistar testes for six and 12 hours. At the time of distortion, decompression fasciotomy was performed on half of the animals.

[Prehospital care to multiple victims with simulated trauma].

Objective: To assess the quality of prehospital care agencies conducted in Vitória, capital of Espírito Santo State, Brazil.

Methods: We conducted a retrospective study in the archives of the League of Academic Surgery and Trauma Care of Espírito Santo (Lacates) regarding 40 victims of a simulated crash between a bus and two cars. The patients were treated by four teams: Military Fire Department of Espírito Santo, Samu 192, County Guard and Civil Defense.

A study of interleukin 6 (IL-6) and tumor necrosis factor alpha (TNF-α) serum levels in rats subjected to fecal peritonitis and treated with intraperitoneal ropivacaine.

Purpose: The objective of this study was to assess the cytokine serum levels of IL-6 and TNF-α in rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine by peritoneal lavage.

Methods: We subjected 16 Wistar rats to laparotomy 6 hours after the induction of fecal peritonitis with autogenous stool and subsequently divided the rats randomly into 4 groups: I-control, no treatment; II- drying of the abdominal cavity; III- lavage of the abdominal cavity with 3 mL of 0.

Histological features of peritoneal lavage with ropivacaine in rats with fecal peritonitis.

Purpose: To evaluate the histological features in lungs, peritoneum and liver of rats subjected to fecal peritonitis and treated with peritoneal lavage with 0.2% ropivacaine.

Methods: Twenty Wistar rats were subjected to laparotomy 6 h after the fecal peritonitis induction with autogenous stool.

A new proposal to evaluate the healing of open skin wounds: volumetry.

Purpose: To present a new proposal to evaluate the healing of an open subcutaneous and skin wound, which we termed "volumetry".

Methods: A total of 32 circular wounds were performed in the subcutaneous tissues and skin of four feet of pigs (8 each). Each wound had about 1 cm in diameter and was 0.

Laparoscopic cholecystectomy in 960 elderly patients.

Objective: To evaluate the operative time, hospital stay, conversion rate, morbidity and mortality in elderly patients undergoing laparoscopic cholecystectomy for gallbladder disease.

Methods: We conducted a descriptive, retrospective study with the chats of 960 patients, aged 60 and 97 years in the period from January 1993 to August 2009.

Results: Operative time ranged from nine to 180 minutes.

Effect of hyperbaric oxygen therapy in rats with subtotal splenectomy preserving the inferior pole.

Purpose: To evaluate the effect of hyperbaric oxygen therapy on the survival and weight of rats submitted to subtotal splenectomy and on the viability and growth of the inferior pole.

Methods: Forty Wistar rats underwent subtotal splenectomy with preservation of the inferior pole and distributed into two groups: Group A (n=20) - not treated with hyperbaric oxygen, Group B (n=20) – treated with hyperbaric oxygen. These groups were divided into two subgroups of 10 animals each, according to the time of euthanasia, 15th and 45th days.

Subtotal laparoscopic splenectomy in rats with preservation of the inferior pole.

Purpose: To evaluate the feasibility and safety of subtotal splenectomy by laparoscopy with inferior pole preservation and to determine the viability of the splenic remnant.

Methods: Twenty male Wistar rats weighing between 365 g and 474 g (mean 417.92 ± 36.

[Splenic auto-implant and hyperbaric oxygen therapy in rats].

Objective: To study the functional and morphological features of splenic autoimplants in rats subjected, or not, to postoperative hyperbaric oxygen therapy.

Methods: One hundred and five male Wistar rats, weighing 251.6 g ± 29.

Venous drainage from the tail of the pancreas to the lienal vein and its relationship with the distal splenorenal shunt selectivity.

Purpose: To identify the veins draining from the pancreatic tail to the lienal vein and its possible relationship with the loss of the distal splenorenal shunt selectivity.

Methods: Thirty eight human blocks including stomach, duodenum, spleen, colon and pancreas, removed from fresh corpses, were studied with the replenish and corrosion technique, using vinilic resin and posterior corrosion of the organic tissue with commercial hydrochloric acid, in order to study the lienal vein and its tributaries.

Results: The number of veins flowing directly to the splenic vein varied from seven to twenty two (14.

Effects of peritoneal lavage with bupivacaine on survival of mice with fecal peritonitis.

Background And Objectives: Based on the knowledge of the anti-inflammatory and anti-bacterial actions of local anesthetics (LA), the objective of this study was to determine the effects of peritoneal lavage with bupivacaine on survival of mice with fecal peritonitis.

Methods: Forty-eight Wistar mice, weighing between 300 and 330 g (311.45 +/- 9.

Epidural anesthesia using a 0,75% ropivacaine and subarachnoid anesthesia with a 0,5% bupivacaine associated or not with clonidine in hemorrhoidectomies.

Purpose: To study the safety and efficiency of two anesthetic blockages in hemorrhoidectomy and the effect of clonidine on analgesia.

Methods: 80 patients were studied, randomly divided into four groups: l (n=19)- peridural with a 0,75% ropivacaine; 2 (n=21)-peridural with a 0,75% ropivacaine and 150 microg of clonidine; 3 (n=19)- subarachnoid with a 0,5% bupivacaine; 4 (n=21)- subarachnoid with a 0,5% bupivacaine and 50 microg of clonidine. The intensity of pain was evaluated by the visual analogical scale 8, 12 and 24 hours after surgery.

[The splenic inferior pole of rats and hyperbaric oxygen].

Objective: To study the functional and morphological features of the lower pole of the spleen in rats submitted, or not, to postoperative hyperbaric oxygen therapy.

Methods: Seventy-nine Wistar rats, weighing 248.7 +/- 27 g, divided into two groups [group A - simulation (n=40), group B - lower pole (n=39)] underwent surgery and were subdivided into two groups: 11 and 70 postoperative days.