Analysis of pentacyclic triterpenoids and phytosterols in cloudberry () by LC-MS/MS.

Cloudberry (.) is a plant rich in various biologically active compounds. In this work, the composition and concentrations of pentacyclic triterpenoids (PCTs) and phytosterols in various parts of cloudberries were studied using high-performance liquid chromatography-tandem mass spectrometry by multiple reaction monitoring mode (MRM, targeted analysis) and precursor ion scan mode (non-targeted analysis and semi-quantitative determination).

Comprehensive identification of polyphenolic metabolites in aspen knotwood by combination of 2D NMR and HPLC-HRMS.

Introduction: European aspen (Populus tremula L.) knotwood contains large amounts of polyphenolic metabolites, mainly flavonoids, and can be considered as a promising industrial-scale source of valuable bioactive compounds. Valorization of knotwood extractives requires detailed information on their chemical composition and a relevant analytical methodology.

Polycommunin A, a dihydrocinnamoyl bibenzyl isolated from the moss and its antioxidant activity.

A novel polyphenolic compound named Polycommunin A (1) was discovered in the aerial part of the common haircap moss () widely spread in boreal and temperate climate zones. Aqueous ethanol and extraction of the plant material with further isolation of polyphenolic fraction and preparative HPLC separation allowed obtaining individual compound and identifying it as dimeric dihydrocinnamoyl bibenzyl by NMR spectroscopy and high-resolution tandem mass spectrometry. Polycommunin A demonstrated high antioxidant activity determined by FRAP and PCL assays and comparable to that of Trolox and Quercetin.

Analysis of Softwood Lignans by Comprehensive Two-Dimensional Liquid Chromatography.

Lignans constitute a large group of phenolic plant secondary metabolites possessing high bioactivity. Their accurate determination in plant extracts with a complex chemical composition is challenging and requires advanced separation techniques. In the present study, a new approach to the determination of lignans in coniferous knotwood extracts as the promising industrial-scale source of such compounds based on comprehensive two-dimensional liquid chromatography separation and UV spectrophotometric detection is proposed.

Fractionation of Arctic Brown Algae () Biomass Using 1-Butyl-3-methylimidazolium-Based Ionic Liquids.

Arctic brown algae are considered a promising industrial-scale source of bioactive sub-stances as polysaccharides, polyphenols, and low-molecular secondary metabolites. Conventional technologies for their processing are focused mainly on the isolation of polysaccharides and involve the use of hazardous solvents. In the present study a "green" approach to the fractionation of brown algae biomass based on the dissolution in ionic liquids (ILs) with 1-butil-3-methylimidazolium (bmim) cation with further sequential precipitation of polysaccharides and polyphenols with acetone and water, respectively, is proposed.

Comprehensive Characterization of Secondary Metabolites in Fruits and Leaves of Cloudberry ( L.).

Cloudberry ( L.) is a circumpolar boreal plant rich in bioactive compounds and is widely used in food and in folk medicine. In this study, a combination of two-dimensional NMR spectroscopy and liquid chromatography-high-resolution mass spectrometry was used for the comprehensive characterization of secondary metabolites in cloudberry lipophilic and hydrophilic extracts.

Determination of Pentacyclic Triterpenoids in Plant Biomass by Porous Graphitic Carbon Liquid Chromatography-Tandem Mass Spectrometry.

Pentacyclic triterpenoids (PCTs), which possess a number of bioactive properties, are considered one of the most important classes of secondary plant metabolites. Their chromatographic determination in plant biomass is complicated by the need to separate a large number of structurally similar compounds belonging to several classes that differ greatly in polarity (monools, diols, and triterpenic acids). This study proposes a rapid, sensitive, and low-cost method for the simultaneous quantification of ten PCTs (3-taraxerol, lupeol, -amyrin, -amyrin, betulin, erythrodiol, uvaol, betulinic, oleanolic, and ursolic acids) by high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS) using porous graphitic carbon (Hypercarb) as a stationary phase capable of hydrophobic retention and specific interactions with analytes.

New Oligomeric Dihydrochalcones in the Moss : Identification, Isolation, and Antioxidant Activity.

One of the most widespread representatives of mosses in the temperate and boreal latitudes of the Northern Hemisphere is common haircap (), which is known as the largest moss in the world and widely used in traditional herbal medicine. Polyphenolic compounds constitute one of the most important groups of biologically active secondary metabolites of , however, the available information on their chemical composition is still incomplete and contradictory. In the present study, a group of dihydrochalcone polyphenolic derivatives that were not previously found in mosses was isolated from biomass using pressurized liquid extraction with aqueous acetone.

Comparative study of four Yucca species by 2D-NMR and LC-MS.

The present study was conducted to compare leaf extracts of and . Plant extracts were obtained by sequential extraction with 50% and 100% methanol using the pressurized liquid extraction method. General comparative analysis of Yucca extracts was performed by 2 D-NMR.

Rapid determination of phthalates in paper products by supercritical fluid chromatography/tandem mass spectrometry.

Phthalic acid esters are widely used as components of industrial and consumer products including paper and cardboard packaging materials in contact with food or human skin. Being endocrine-disrupting chemicals, phthalic acid esters have a negative effect on human health and must be controlled in pulp and paper products. In the present study, supercritical fluid chromatography-tandem mass spectrometry in combination with pressurized liquid extraction was proposed for phthalic acid esters determination in such objects.

Rapid Simultaneous Quantification of 1-Formyl-2,2-Dimethylhydrazine and Dimethylurea Isomers in Environmental Samples by Supercritical Fluid Chromatography-Tandem Mass Spectrometry.

When released to the environment, the rocket fuel unsymmetrical dimethylhydrazine (UDMH) undergoes oxidative transformations, resulting in the formation of an extremely large number of nitrogen-containing transformation products, including isomeric compounds which are difficult to discriminate by common chromatography techniques. In the present work, supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS) was proposed for resolving the problem of fast separation and simultaneous quantification of 1-formyl-2,2-dimethylhydrazine (FADMH) as one of the major UDMH transformation products, and its isomers-1,1-dimethylurea (UDMU) and 1,2-dimethylurea (SDMU). 2-Ethylpyridine stationary phase provided baseline separation of analytes in 1.

Supercritical Fluid Chromatography-Tandem Mass Spectrometry for Rapid Quantification of Pentacyclic Triterpenoids in Plant Extracts.

Pentacyclic triterpenoids (PCTs) are a widely distributed class of plant secondary metabolites. These compounds have high bioactive properties, primarily antitumor and antioxidant activity. In this study, a method was developed for the quantitative analysis of pentacyclic triterpenoids in plants using supercritical fluid chromatography-tandem mass spectrometry (SFC-MS/MS).

Analysis of the functional group composition of the spruce and birch phloem lignin.

In this article, the functional group composition of the spruce (Pícea ábies) and birch (Bétula péndula) phloem lignin is described. The features of the chemical structure were studied by analyzing dioxane lignin using the elemental analysis, UV-Vis, FT-IR, and 1D NMR spectroscopy. For comparison, samples of xylem dioxane lignin isolated from the corresponding wood species were also analyzed.

Screening and semi-quantitative determination of pentacyclic triterpenoids in plants by liquid chromatography-tandem mass spectrometry in precursor ion scan mode.

Introduction: Pentacyclic triterpenoids (PCTs) are secondary plant metabolites. They are of exceptional interest as biologically active substances and raw materials for a wide range of medications. Thus, the development of a methodology for rapid screening of PCTs in plant biomass is an important task.

Data on the spatial distribution of 1,1-dimethylhydrazine and its transformation products in peat bog soil of rocket stage fall site in Russian North.

The data set covers the results of a study of 96 samples of peat bog soil from the fall place of the first stage of the Cyclone-3 launch vehicle contained unburned toxic rocket fuel 1,1-dimethylhydrazine (UDMH) in the European North of Russia. Soil samples were taken during a helicopter expedition to the "Koida" fall region of Plesetsk Cosmodrome operation zone in October 2015 at different distances from the center of the fall site and from different soil horizons. Samples were analyzed by liquid chromatography with amperometric detection and gas chromatography - tandem mass spectrometry.

Migration and transformation of 1,1-dimethylhydrazine in peat bog soil of rocket stage fall site in Russian North.

An ingress of highly toxic rocket fuel 1,1-dimethylhydrazine (UDMH) and its transformation products into environment represents a serious negative impact on the ecosystem, as well as human health. The present research demonstrates the first data on the spatial distribution and quantification of UDMH and its main transformation products (methylhydrazine, hydrazine, 1,1,4,4-tetramethyltetrazene, formaldehyde, acetaldehyde and furaldehyde N,N-dimethylhydrazones, 1-formyl-2,2-dimethylhydrazine, N,N-dimethylformamide, N-nitrosodimethylamine, and 1-methyl-1H-1,2,4-triazole) in the peat bog soil of the fall site in subarctic region. One hundred samples of peat bog soil and one sample of surface water were analyzed by the developed earlier methodology.

Evaluation of temperature and pressure effects on retention in supercritical fluid chromatography on polar stationary phases.

Four polar stationary phases (ethylene-bridged hybrid silica, cyanopropyl, 2-ethylpyridine, and zwitterionic sulfobetaine) have been characterized in supercritical fluid chromatography (SFC) by linear free energy relationships (LFER) method with an extended set of Abraham's descriptors. Temperature (25-55 °C) and pressure (110-180 bar) effects on analyte retention, separation selectivity and LFER-coefficients of chromatographic systems have been studied using the 89 test compounds of various chemical classes and carbon dioxide - methanol (9:1 v/v) binary solvent as a mobile phase. It was found that for the selected stationary phases temperature and pressure had only moderate effects on selectivity.