Publications by authors named "Danielle M Agnello"
Background: Co-creation is increasingly recognized for its potential to generate innovative solutions, particularly in addressing complex and wicked problems in public health. Despite this growing recognition, there are no standards or recommendations for method use in co-creation, leading to confusion and inconsistency. While some studies have examined specific methods, a comprehensive overview is lacking, limiting the collective understanding and ability to make informed decisions about the most appropriate methods for different contexts and research objectives.
View Article and Find Full Text PDFBackground: Co-creation is seen as a way to ensure all relevant needs and perspectives are included and to increase its potential for beneficial effects and uptake process evaluation is crucial. However, existing process evaluation frameworks have been built on practices characterised by top-down developed and implemented interventions and may be limited in capturing essential elements of co-creation. This study aims to provide a review of studies planning and/or conducting a process evaluation of public health interventions adopting a co-creation approach and aims to derive assessed process evaluation components, used frameworks and insights into formative and/or participatory evaluation.
View Article and Find Full Text PDFArticle Synopsis
- Co-creation is an effective approach for addressing complex public health issues, but there is a lack of guidance on how to effectively plan and implement it.
- The study analyzed existing public health implementation and evaluation frameworks through a systematic review, identifying key steps and data for effective co-creation.
- Recommendations include starting implementation considerations early, using a systems thinking approach, involving stakeholders, and employing an iterative evaluation design focused on co-creator experiences.
Conducting co-creation for public health in low and middle-income countries: a systematic review and key informant perspectives on implementation barriers and facilitators.
Global Health
January 2024
Background: There has been an increase in the use of co-creation for public health because of its claimed potential to increase an intervention's impact, spark change and co-create knowledge. Still, little is reported on its use in low-and-middle-income countries (LMICs). This study offers a comprehensive overview of co-creation used in public-health-related interventions, including the interventions' characteristics, and reported implementation barriers and facilitators.
View Article and Find Full Text PDFBackground: Co-creation is an approach that aims to democratize research and bridge the gap between research and practice, but the potential fragmentation of knowledge about co-creation has hindered progress. A comprehensive database of published literature from multidisciplinary sources can address this fragmentation through the integration of diverse perspectives, identification and dissemination of best practices, and increase clarity about co-creation. However, two considerable challenges exist.
View Article and Find Full Text PDFArticle Synopsis
- The type VI secretion system (T6SS) in Gram-negative bacteria requires precise mechanisms to identify and select substrate proteins from the cellular pool.
- Haemolysin coregulated protein (Hcp), a key component of T6SS, has been found to specifically bind to effector molecules, indicating its role as a chaperone and receptor rather than just a passive conduit.
- This research reveals that the interaction between Hcp and effectors is essential for the secretion process and highlights significant differences between T6SS and other secretory pathways.
Article Synopsis
- Membranes are essential for life as they compartmentalize biochemical processes and are common targets for antagonistic interactions between organisms.
- Researchers discovered a diverse superfamily of bacterial phospholipase enzymes, including type VI lipase effectors that play a role in mediating bacterial interactions via the type VI secretion system (T6SS).
- One such enzyme, PldA from Pseudomonas aeruginosa, not only contributes to pathogenesis but also functions as an antibacterial agent by degrading phosphatidylethanolamine, highlighting the complex interplay between bacterial interactions and the evolution of virulence.
Interbacterial interaction pathways play an important role in defining the structure and complexity of bacterial associations. A quantitative description of such pathways offers promise for understanding the forces that contribute to community composition. We developed time-lapse fluorescence microscopy methods for quantitation of interbacterial interactions and applied these to the characterization of type VI secretion (T6S) in Pseudomonas aeruginosa.
View Article and Find Full Text PDFArticle Synopsis
- Sophisticated T6SS in Pseudomonas aeruginosa helps bacteria exchange information by delivering antibiotic effectors that target cell walls of neighboring bacteria.
- A wide variety of peptidoglycan-degrading enzymes have been identified as T6SS effectors, which are crucial for bacterial competition.
- These effectors are linked with immunity proteins to prevent self-harm, highlighting the evolutionary pressure for such systems in different environments, including those relevant to human health.