Integrating single nuclei and bulk RNA sequencing in rice shoot apical meristems uncovers candidate early floral transition gene networks.

We describe transcriptional dynamics at the rice shoot apex by integrating time resolved single nuclei RNA-seq with bulk RNA-seq data. In rice, short days trigger floral transition and the transcriptional reprogramming of the shoot apex to become reproductive. We integrated time-resolved bulk RNA-seq with single nuclei RNA-seq analysis to gain a refined understanding of the transcriptional programs induced at the shoot apex during floral transition.

NDUFS3 knockout cancer cells and molecular docking reveal specificity and mode of action of anti-cancer respiratory complex I inhibitors.

Inhibition of respiratory complex I (CI) is becoming a promising anti-cancer strategy, encouraging the design and the use of inhibitors, whose mechanism of action, efficacy and specificity remain elusive. As CI is a central player of cellular bioenergetics, a finely tuned dosing of targeting drugs is required to avoid side effects. We compared the specificity and mode of action of CI inhibitors metformin, BAY 87-2243 and EVP 4593 using cancer cell models devoid of CI.