General aspects, host interaction, and application of sp. in arthropod pest and vector control.

The application of microorganisms as bio-control agents against arthropod populations is a need in many countries, especially in tropical, subtropical, and neotropical endemic areas. Several arthropod species became agricultural pests of paramount economic significance, and many methods have been developed for field and urban applications to prevent their, the most common being the application of chemical insecticides. However, the indiscriminate treatment based upon those substances acted as a selective pressure for upcoming resistant phenotype populations.

Immune signaling pathways in in the context of infection: cellular and humoral immune responses and microbiota modulation.

Introduction: is a hematophagous insect and one of the main vectors for and parasites in Latin America. Gut microbiota and insect immune responses affect and infection within triatomines. Particularly the Toll and IMD signaling pathways activations and how they orchestrate the antimicrobial peptides (AMPs) expressions in , especially when infected by .

The Development of a One-Step RT-qPCR for the Detection and Quantification of Viable Forms of in Açai Samples from Areas at Risk of Chagas Disease through Oral Transmission.

Currently, approximately 70% of new cases of Chagas disease (CD) in Brazil are attributed to oral transmission, particularly through foods such as açaí, bacaba, and sugarcane juice, primarily in the northern and northeastern regions of the country. This underscores the imperative need to control the spread of the disease. The methods utilized to conduct quality control for food associated with outbreaks and to assess the potential for the oral transmission of CD through consuming açaí primarily rely on isolating the parasite or inoculating food into experimental animals, restricting the analyses to major research centers.

Digestive α-L-fucosidase activity in after blood feeding: effect of secretagogue and nutritional stimuli.

(Hemiptera: Reduviidae) is an important vector of , the causative agent of Chagas Disease. This insect is a model for the study of insect physiology, especially concerning the digestion of blood. Among the enzymes produced in the midgut of after blood feeding there is a α-L-fucosidase activity.

RNA as a feasible marker of Trypanosoma cruzi viability during the parasite interaction with the triatomine vector Rhodnius prolixus (Hemiptera, Triatominae).

A recurring question concerning Trypanosoma cruzi DNA detection/quantification is related to the fact that DNA amplification, by itself, does not differentiate between viable or dead parasites. On the other hand, RNA can be considered a potential molecular marker of pathogens viability. Herein, we developed a quantitative real-time PCR with reverse Transcription (RT-qPCR) to quantify viable T.

Trypanocidal Activity, Genomic Analysis of Isolates, and Transcription of Type VI Secretion System of Belonging to the Microbiota of Digestive Tract.

is a bacterium with the ability to colonize several niches, including some eukaryotic hosts. have been recently found in the gut of hematophagous insects that act as parasite vectors, such as , and . While some strains have been reported as symbiotic or pathogenic to other insects, the role of populations from the gut microbiota of , a vector of Chagas' disease, remains unknown.

Characterization of the microbiota in the guts of Triatoma brasiliensis and Triatoma pseudomaculata infected by Trypanosoma cruzi in natural conditions using culture independent methods.

Background: Chagas disease is caused by Trypanosoma cruzi, which is transmitted by triatomine vectors. The northeastern region of Brazil is endemic for Chagas disease and has the largest diversity of triatomine species. T.