Publications by authors named "Daniele Joseleau-Petit"
YajL is the most closely related Escherichia coli homolog of Parkinsonism-associated protein DJ-1, a protein with a yet-undefined function in the oxidative-stress response. YajL protects cells against oxidative-stress-induced protein aggregation and functions as a covalent chaperone for the thiol proteome, including FeS proteins. To clarify the cellular responses to YajL deficiency, transcriptional profiling of the yajL mutant was performed.
View Article and Find Full Text PDFLuria-Bertani broth supports Escherichia coli growth to an optical density at 600 nm (OD(600)) of 7. Surprisingly, however, steady-state growth ceases at an OD(600) of 0.3, when the growth rate slows down and cell mass decreases.
View Article and Find Full Text PDFArticle Synopsis
- The study investigates the characteristics of bacterial L forms, known to typically lack peptidoglycan, particularly focusing on how they divide.
- Using the beta-lactam cefsulodin, researchers turned wild Escherichia coli K-12 into a state resembling L forms, finding these cells to be spherical and sensitive to osmotic changes.
- Despite appearances, further analysis revealed that these L-form-like cells still require components linked to peptidoglycan synthesis for growth and division, suggesting that even in this altered state, they retain a reduced form of peptidoglycan that is critical for cell division.