Detrusor bioengineering using a cell-enriched compressed collagen hydrogel.

Objective: The gold standard for bladder regeneration in end-stage bladder disease is the use of intestinal tissue, which is however associated with significant long-term complications. Our study aims to bioengineer functional detrusor muscle combining bladder smooth muscle cells (SMC) and SMC-like adipose-derived stem cells (pADSC) in compressed collagen (CC) hydrogels and to investigate biocompatibility and tissue regeneration of such detrusor-equivalents in a rat detrusorectomy model.

Methods: Compressed collagen hydrogels seeded with 1 × 10 or 4 × 10 SMC alone or in combination with pADSC in a 1:1 ratio were investigated.

Angiotensin 1-7 significantly reduces diabetes-induced leukocyte recruitment both in vivo and in vitro.

Objective: Recent studies have demonstrated that Ang1-7 has anti-inflammatory effects. Since the formation of Ang1-7 is significantly altered in the setting of diabetes, here we aimed to evaluate whether Ang1-7 infusion could ameliorate diabetes-induced leukocyte recruitment.

Methods: Wild-type male Wistar rats were randomly allocated to the following groups: control + saline, control + Ang1-7, diabetes + saline, diabetes + Ang1-7.