Application of Cloning-Free Genome Engineering to .

The propagation of foreign DNA in is central to molecular biology. Recent advances have dramatically expanded the ability to engineer (bacterial) cells; however, most of these techniques remain time-consuming. The aim of the present work was to explore the possibility to use the cloning-free genome editing (CFGE) approach, proposed by Döhlemann and coworkers (2016), for genetics, and to deepen the knowledge about the homologous recombination mechanism.