Publications by authors named "Daniela Yumi Takata"
Article Synopsis
- Tetanus toxin (TeNT) is created by a widespread spore-forming bacillus, and although there's a safe vaccine, tetanus remains common in low-income countries due to insufficient booster shots and varying vaccine responses.* -
- Researchers identified a set of monoclonal antibodies (mAbs) that can neutralize TeNT by preventing its binding to neurons and showed that a combination of three specific mAbs can protect mice from the toxin's effects.* -
- They developed stable cell lines to produce large quantities of these mAbs and conducted various tests (like ELISA and surface plasmon resonance) to confirm the antibodies' effectiveness in binding to TeNT and inhibiting its activity.*
Potency testing of tetanus antitoxin must be performed in vivo, in a very painful, stressful and prone to high variability assay. It is, therefore, mandatory to find alternatives to this kind of potency assessment. Immunochemical tests as ELISA or ToBI test are already available but usually results in a poor correlation to the in vivo protection.
View Article and Find Full Text PDFThe use of antibody-based therapy to treat a variety of diseases and conditions is well documented. The use of antibodies as an antidote to treat tetanus infections was one of the first examples of immunotherapy and remains the standard of care for cases involving potential infections. Plasma-derived immunoglobulins obtained from human or horse pose risks of infection from undetectable emergent viruses or may cause anaphylaxis.
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