Metabolic folate profiling as a function of time during cultivation suggests potential C2-metabolism in .

Yeasts are reported to be rich in folates, a group of vitamers known to be involved in several biosynthetic reactions such as methylation reactions, oxidation and reduction processes, and nucleotide synthesis. Not being able to synthesize folates, humans rely on external folate supply. Here, we show the application of LC/MS-MS methods using SIDA (stable isotope dilution analysis) assays for the quantitative analysis of different folate mono- and polyglutamates during growth of .

Comprehensive Vitamer Profiling of Folate Mono- and Polyglutamates in Baker's Yeast () as a Function of Different Sample Preparation Procedures.

Folates are a group of B vitamins playing an important role in many metabolic processes such as methylation reactions, nucleotide synthesis or oxidation and reduction processes. However, humans are not able to synthesize folates de novo and thus rely on external sources thereof. Baker's yeast () has been shown to produce high amounts of this vitamin but extensive identification of its folate metabolism is still lacking.

Optimization of the marginal gap of all-ceramic restorations and gold crowns by ultrasound activated, suspended Al2O3 powder.

Purpose: To evaluate if the use of ultrasound activated Al203 powder improved the fit especially of all-ceramic restorations.

Methods: 21 extracted teeth were prepared for 14 all-ceramic IPS Empress2 (IPS Empress2) restorations (four crowns, four partial crowns, two inlays, four veneers) and seven gold crowns (Degudent H). Each restoration was adjusted with a suspension of Al2O3 of 25 microm and 50 microm grit, respectively.