Encapsulation, Shelf Life, and Virulence of sp. Against .

is a genus of entomophthoralean fungi often associated with insect epizootics, particularly in phytophagous hemipterans. Encapsulation has become a promising strategy for improving the shelf life and sporulation of these fungi post-application. This study aims to (i) compare the virulence of the submerged propagules and primary conidia of sp.

Genome sequences of four novel strains associated with a tropical octocoral in a long-term aquarium facility.

We report the genome sequences of four sp. strains isolated from the octocoral maintained long term at an aquarium facility. Our analysis reveals the coding potential for versatile polysaccharide metabolism; Type II, III, IV, and VI secretion systems; and the biosynthesis of novel ribosomally synthesized and post-translationally modified peptides.

CFAM 521 Isolated From the Amazon Region: A Novel Source of a Fibrinolytic Enzyme.

Fibrinolytic agents are essential in treating thrombosis, playing a critical role in improving survival rates in cardiovascular diseases. Microbial fibrinolytic proteases have emerged as promising alternatives due to their affordability, specificity, lower toxicity, and reduced side effects. Consequently, the search for microorganisms capable of producing these enzymes has gained significant economic importance in the pharmaceutical industry.

Phytochemical and antimicrobial evaluation of Triana (Melastomataceae).

Four novel triterpenoid saponins, 1,3,23-trihydroxy-olean-12-en-28-oic acid 28---glycopyranoside ester (), 1,3,23-trihydroxy-urs-12-en-28-oic acid 28---glycopyranoside ester (), 3---galloyl-1,23-dihydroxy-olean-12-en-28-oic acid 28---glycopyranoside ester () and 3---galloyl-1,23-dihydroxy-urs-12-en-28-oic acid 28---glycopyranoside ester () and two new pentacyclic triterpenoids, 3-----coumaroyl-1-hydroxy-urs-12-en-28-oic acid () and 3-----coumaroyl-1-hydroxy-urs-12-en-28-oic acid (), together with six known compounds were isolated from the ethanolic extracts of leaves and stem bark. Their structures were established by HR-MS, 1D and 2D NMR and comparison with literature data. Additionally, the antimicrobial activity of the leaves and stem bark extracts and fractions was evaluated using the micro broth dilution technique.

Biocompatible adipose extracellular matrix and reduced graphene oxide nanocomposite for tissue engineering applications.

Despite the immense need for effective treatment of spinal cord injury (SCI), no successful repair strategy has yet been clinically implemented. Multifunctional biomaterials, based on porcine adipose tissue-derived extracellular matrix (adECM) and reduced graphene oxide (rGO), were recently shown to stimulate neural stem cell growth and differentiation. Nevertheless, their functional performance in clinically more relevant conditions remains largely unknown.

Developing Pharmacological Therapies for Atrial Fibrillation Targeting Mitochondrial Dysfunction and Oxidative Stress: A Scoping Review.

Atrial fibrillation (AF) is a cardiac arrhythmia caused by electrophysiological anomalies in the atrial tissue, tissue degradation, structural abnormalities, and comorbidities. A direct relationship exists between AF and altered mitochondrial activity resulting from membrane potential loss, contractile dysfunction, or decreased ATP levels. This review aimed to elucidate the role of mitochondrial oxidative mechanisms in AF pathophysiology, the impact of mitochondrial oxidative stress on AF initiation and perpetuation, and current therapies.

Production of and by Submerged Liquid Fermentation and Bioactivity against and through Seed Inoculation.

Pochoniachlamydosporia and Purpureocilliumlilacinum are fungal bioagents used for the sustainable management of plant parasitic nematodes. However, their production through submerged liquid fermentation and their use in seed treatment have been underexplored. Therefore, our goal was to assess the effect of different liquid media on the growth of 40 isolates of P.

Vogel (Fabaceae): phytochemical study and toxicity to Linnaeus (Hymenoptera: Formicidae).

In this work was described the phytochemical investigation of Vogel (Fabaceae) leaves as well as the insecticidal activity of its crude extract and fractions against Linnaeus (Hymenoptera: Formicidae). The phytochemical study led to the identification of α-amyrin, β-amyrin, lupeol, phytol, isomucronulatol and rutin, described for the first time in and mucronulatol. Insecticidal activity was assessed by the ingestion of the crude extract and fractions incorporated into an artificial diet at three different concentrations (0.

Decreasing sperm quality in mice subjected to chronic cannabidiol exposure: New insights of cannabidiol-mediated male reproductive toxicity.

Cannabidiol (CBD) is a natural cannabinoid present in the Cannabis sativa plant, widely prescribed as an anticonvulsant drug, especially for pediatric use. However, its effects on male reproduction are still little investigated. Therefore, the present study assessed the effects of CBD on the spermatogenesis and sperm quality.

Prevalence of depressive and anxiety disorders in patients with glaucoma: a cross-sectional study.

Purpose: Our goal was to analyze the prevalence of depression and anxiety among patients with glaucoma and to identify risk factors related to these disorders.

Methods: A cross-sectional study was carried out between August 2016 and August 2017 at the Hospital das Clínicas of Universidade Estadual de Campinas and at the Hospital Oftalmológico de Brasília to evaluate the prevalence of depressive and anxiety disorders among patients diagnosed with glaucoma. All patients underwent a complete ophthalmologic examination with standard automated perimetry to confirm the diagnosis of glaucoma.

Evaluation of Respiratory Muscular Strength Compared to Predicted Values in Patients with Stroke.

The purpose of this study was to evaluate the inspiratory and expiratory muscle strength of individuals affected by stroke and to compare it with the predicted values in the literature considering their corresponding age. Respiratory muscle strength was evaluated in 22 elderly people who had sequels of stroke, four with right hemiparesis, 16 with left hemiparesis and two with bilateral, of ages ranging from 34 to 82 years. The collected data were submitted to statistical analysis using a Mann-Whitney test to evaluate if there was a significant difference in the average data collected when compared with a mean of the predicted data in the literature.

Evaluation of genotoxic and cytotoxic effects in buccal mucosa cells of welders in the cities of Cubatão and Santos, state of São Paulo, Brazil.

Background: Approximately 5 million workers are estimated to be occupationally exposed to welding fumes worldwide. Nickel and chromium are genotoxic metals found in welding fumes, therefore welders are exposed to these metals at the workplace.

Objective: The objective of the present study was to investigate the frequency of cytotoxic and genetic damage in cells harvested from the oral mucosa of welders and also from a group of workers not exposed to metallic fumes.

Influence of hydrological pathways on dissolved organic carbon fluxes in tropical streams.

Water flow pathways and water balance are fundamental components for understanding the dynamics of C in the soil/water interface of small basins. The objective of this study was to describe the seasonal variations and estimate the annual balance of dissolved organic carbon (DOC) by comparing two tropical microbasins (preserved forest-PF and cacao plantation-CP). Twenty-one weekly collections were conducted from September to December 2012 and from April to June 2013.

Evaluation of nutrients and major ions in streams-implications of different timescale procedures.

Small watersheds are characterized by a high degree of sensitivity to changes observed in their environment, making them important sampling and management units. Due to this high sensitivity, several studies have shown that intensive collecting may be more effective in these systems compared to other timescale procedures. The aim of this study was to evaluate the concentration of organic and inorganic nutrients and major ions dissolved in two small watersheds with different land uses to determine whether there are differences between these watersheds with different levels of impact and to identify the most appropriate timescale procedure for the variables under analysis.

Detection of CDT toxin genes in Campylobacter spp. strains isolated from broiler carcasses and vegetables in São Paulo, Brazil.

Campylobacteriosis is a worldwide distributed zoonosis. One of the main virulence factors related to Campylobacter spp. in animals and humans is the cytolethal distending toxin (CDT), encoded by three adjacent genes (cdtA, cdtB, cdtC).

Influence of land use changes on water chemistry in streams in the State of São Paulo, southeast Brazil.

Streamwater is affected by several processes in the watershed including anthropogenic activities that result in changes in water quality as well as in the functioning of these stream ecosystems. Therefore, this work aims to evaluate the concentration of major ions (Ca(2+), Mg(2+), Na(+), K(+), NH4(+), NO3(-), NO2(-), Cl(-), SO4(2-), PO4(3-), HCO3(-)) in streams in the state of São Paulo (southeast Brazil). The sampling sites are located at undisturbed (ombrophilus dense forest, semideciduous forest and savanna - cerrado) and disturbed areas (pasture, urbanization and sugar cane crops).

Characterization of the mechanical and biological properties of a new alumina scaffold.

Purpose: In this work, an alumina scaffold was produced through a new method to be used in a near future as a bone substitute.

Methods: In vitro and in vivo studies were performed in order to characterize the mechanical and biological properties of the scaffold.

Results: The results obtained showed that this scaffold has high mechanical resistance and a porous surface that allows human osteoblast cells to adhere and proliferate.

Challenges in clinical and laboratory diagnosis of androgen insensitivity syndrome: a case report.

Introduction: Androgen is a generic term usually applied to describe a group of sex steroid hormones. Androgens are responsible for male sex differentiation during embryogenesis at the sixth or seventh week of gestation, triggering the development of the testes and penis in male fetuses, and are directed by the testicular determining factor: the gene SRY (sex determining region on Y chromosome) located on the short arm of chromosome Y. The differentiation of male external genitalia (penis, scrotum and penile urethra) occurs between the 9th and 13th weeks of pregnancy and requires adequate concentration of testosterone and the conversion of this to another more potent androgen, dihydrotestosterone, through the action of 5α-reductase in target tissues.

[Development and reproduction of Sipha flava (Forbes) (Hemiptera: Aphididae) at different temperatures].

The aphid Sipha flava (Forbes) is a pest on elephant grass, but little is known about its biology. The objective of this work was to evaluate the temperature effects on the development, survival and reproduction of S. flava fed on Pennisetum purpureum.