Virulence and resistance patterns of non-O1/non-O139 acquired in Germany and other European countries.

Global warming has caused an increase in the emergence of species in marine and estuarine environments as well as fresh water bodies. Over the past decades, antimicrobial resistance (AMR) has evolved among species toward various antibiotics commonly used for the treatment of infections. In this study, we assessed virulence and resistance patterns of non-O1/non-O139 strains derived from Germany and other European countries.

Ulceroglandular form of tularemia after squirrel bite: a case report.

Background: The diagnosis of tularemia is not often considered in Germany as the disease is still rare in this country. Nonetheless, Francisella tularensis, the causative agent of tularemia, can infect numerous animal species and should, therefore, not be neglected as a dangerous pathogen. Tularemia can lead to massively swollen lymph nodes and might even be fatal without antibiotic treatment.

Adaptation of Antimicrobial Susceptibility Testing to the ISO 20776 Standard and Validation of the Method.

Brucellosis, mainly caused by (B.) , is associated with a risk of chronification and relapses. Antimicrobial susceptibility testing (AST) standards for are not available, and the agent is not yet listed in the EUCAST breakpoint tables.

Epidemiological investigation of a tularaemia outbreak after a hare hunt in Bavaria, Germany, 2018.

In November 2018, a tularaemia outbreak occurred in Bavaria, Germany, among participants of a hare hunt and butchery employees handling the hares. We conducted an epidemiological outbreak investigation, including a retrospective cohort study among hunting participants, to identify likely transmission routes and activities associated with infection. Twelve of 41 participants were antibody-positive for Francisella (F.

Heatwave-associated infections in Germany, 2018 and 2019.

Background spp. are aquatic bacteria that prefer warm seawater with moderate salinity. In humans, they can cause gastroenteritis, wound infections, and ear infections.

Complete Genome Sequence of Francisella tularensis subsp. Strain A271_1 (FDC408), Isolated from a Eurasian Beaver (Castor fiber).

Here, we report the complete genome sequence of subsp. strain A271_1, isolated from a Eurasian beaver () in 2012 in the Berlin/Brandenburg region, Germany.

Outbreak of Tularemia in a Group of Hunters in Germany in 2018-Kinetics of Antibody and Cytokine Responses.

In November 2018, an outbreak of tularemia occurred among hare hunters in Bavaria, Germany. At least one infected hare was confirmed as the source of infection. A number of hunting dogs showed elevated antibody titers to , but the absence of titer increases in subsequent samples did not point to acute infections in dogs.

Subspecies and Tularemia in Germany.

Tularemia is a zoonotic disease caused by a small, pleomorphic, facultative intracellular bacterium. In Europe, infections in animals and humans are caused mainly by subspecies . Humans can be exposed to the pathogen directly and indirectly through contact with sick animals, carcasses, mosquitoes and ticks, environmental sources such as contaminated water or soil, and food.

Assessment of biorisk management systems in high containment laboratories, 18 countries in Europe, 2016 and 2017.

Europe-wide activities to improve biosafety and biosecurity performed within the frameworks of the European Union (EU)-funded Joint Actions EMERGE and QUANDHIP led to the development of an Integrated European Checklist for Laboratory Biorisk Management (ECL).To better understand different approaches shaping biorisk management (BRM) systems on an operational level in high containment laboratories, the ECL was used to map the implementation of BRM in 32 high containment laboratories in 18 countries in Europe. The results suggest that the BRM elements referring to standard microbiological working practices and the handling of infectious material were fulfilled particularly well.

The Phages ΦE058 and ΦE067 Represent Distinct Prototypes of a New Subgroup of Temperate Myoviruses.

and are highly pathogenic species which are closely related, but diverse regarding their prophage content. While temperate phages have not yet been isolated from , several phages of , and its non-pathogenic relative have been described. In this study we isolated two phages from and three phages from and determined their morphology, host range, and relationship.

Genetic Diversity and Spatial Segregation of Subspecies in Germany.

is an intracellular pleomorphic bacterium and the causative agent of tularemia, a zoonotic disease with a wide host range. Among the subspecies, especially subsp. is of clinical relevance for European countries.

Clinical characteristics in a sentinel case as well as in a cluster of tularemia patients associated with grape harvest.

Background: Tularemia is caused by Francisella tularensis and can occasionally establish foodborne transmission.

Methods: Patients were identified by active case detection through contact with the treating physicians and consent for publication was obtained. Clinical data were accumulated through a review of the patient charts.

Molecular identification of the source of an uncommon tularaemia outbreak, Germany, autumn 2016.

BackgroundIn 2016, an uncommon outbreak of oropharyngeal tularaemia involving six human cases occurred in Germany, caused by drinking contaminated fresh must after a grape harvest.AimWe describe the details of laboratory investigations leading to identification of the outbreak strain, its characterisation by next generation sequencing (NGS) and the finding of the possible source of contamination.MethodsWe incubated wine samples in different media and on agar plates.

Draft Genome Sequence of Bacillus anthracis Strain Sterne 09RA8929.

A Bacillus anthracis vaccine strain (Sterne), used as an attenuated laboratory comparative strain, was sequenced and analyzed. A comparison to assemblies of B. anthracis strain Sterne (NZ_CP009541 and NZ_CP009540) was performed.

Population Genomics of subsp. and its Implication on the Eco-Epidemiology of Tularemia in Switzerland.

Whole genome sequencing (WGS) methods provide new possibilities in the field of molecular epidemiology. This is particularly true for monomorphic organisms where the discriminatory power of traditional methods (e.g.

Tularemia in Germany-A Re-emerging Zoonosis.

Tularemia, also known as "rabbit fever," is a zoonosis caused by the facultative intracellular, gram-negative bacterium . Infection occurs through contact with infected animals (often hares), arthropod vectors (such as ticks or deer flies), inhalation of contaminated dust or through contaminated food and water. In this review, we would like to provide an overview of the current epidemiological situation in Germany using published studies and case reports, an analysis of recent surveillance data and our own experience from the laboratory diagnostics, and investigation of cases.

Prioritization of High Consequence Viruses to Improve European Laboratory Preparedness for Cross-Border Health Threats.

Highly infectious diseases can spread rapidly across borders through travel or trade, and international coordination is essential to a prompt and efficient response by public health laboratories. Therefore, developing strategies to identify priorities for a rational allocation of resources for research and surveillance has been the focus of a large body of research in recent years. This paper describes the activities and the strategy used by a European-wide consortium funded by the European Commission, named EMERGE (Efficient response to highly dangerous and emerging pathogens at EU level), for the selection of high-threat pathogens with cross-border potential that will become the focus of its preparedness activities.

Brucellosis in a refugee who migrated from Syria to Germany and lessons learnt, 2016.

A teenage woman migrating from Syria arrived in May 2015 in Germany. She gave birth to a healthy child in early 2016, but became febrile shortly after delivery. Blood cultures revealed Brucella melitensis.

Characterization of Vibrio cholerae Strains Isolated from the Nigerian Cholera Outbreak in 2010.

We examined clinical samples from Nigerian patients with acute watery diarrhea for Vibrio cholerae during the 2010 cholera outbreak. A total of 109 suspected isolates were characterized, but only 57 V. cholerae strains could be confirmed using multiplex real-time PCR as well as rpoB sequencing and typed as V.

Imported cholera with acute renal failure after a short business-trip to the Philippines, Germany, October 2015.

A German businessman developed acute watery diarrhoea after a three-day trip to the Philippines. He was admitted with severe hypotension and acute renal failure, but recovered with rapid rehydration. Vibrio cholerae O1 serotype Ogawa was isolated.

Successful re-evaluation of broth medium T for growth of Francisella tularensis ssp. and other highly pathogenic bacteria.

Pavlovich's medium T was compared with other broadly used media and extensively checked by growth of various subspecies of Francisellatularensis as well as other risk group 3 bacteria. The medium was successfully re-evaluated as an optimal liquid medium suitable for enrichment of fastidious and/or highly pathogenic bacteria.