Comparative in vitro and in silico analysis of the ability of basic Asp49 phospholipase A and Lys49-phospholipase A-like myotoxins from Bothrops diporus venom to inhibit the metastatic potential of murine mammary tumor cells and endothelial cell tubulogenesis: Asp49 vs Lys49 phospholipases A: Inhibition of metastasis and angiogenesis.

Snake venoms are a complex mixture of proteins and polypeptides that represent a valuable source of potential molecular tools for understanding physiological processes for the development of new drugs. In this study two major PLAs, named PLA-I (Asp49) and PLA-II (Lys49), isolated from the venom of Bothrops diporus from Northeastern Argentina, have shown cytotoxic effects on LM3 murine mammary tumor cells, with PLA-II-like exhibiting a stronger effect compared to PLA-I. At sub-cytotoxic levels, both PLAs inhibited adhesion, migration, and invasion of these adenocarcinoma cells.

Traces of Bothrops snake venoms in necrotic muscle preclude myotube formation in vitro.

Deficient skeletal muscle regeneration, which often leads to permanent sequelae, is a common clinical finding in envenomations caused by snakes of the family Viperidae, such as those of Bothrops alternatus and B. diporus in South America. The causes of such poor muscle regenerative outcome are still incompletely understood.