Investigating the safety of photobiomodulation in oral carcinogenesis: insights into cell proliferation, invasion, and apoptosis via the 4NQO model.

The present investigation aimed to assess the safety of photobiomodulation (PBM) on the oral carcinogenesis process induced by 4NQO, focusing on cell proliferation and apoptosis. Sixty-six Wistar rats received systemic 4NQO for 12 (n = 33) and 20 weeks (n = 33), divided into Control group, PBM 0.3 J, and PBM 1 J.

Proposal of a secure and efficient protocol for a murine oral carcinogenesis model induced by 4-nitroquinoline-1-oxide (4NQO).

An important rat model using the chemical carcinogen 4-nitroquinoline-1-oxide (4NQO) has been described for the study of the process of oral carcinogenesis. This model replicates the gradual progression seen in oral carcinoma patients. However, due to its high level of toxicity, its use in fundamental research is challenging.

Biodistribution of Transplanted Hematopoietic Precursor Cells Injected Through Different Administration Routes in Newborn Mice.

Hematopoietic stem cell transplantation has been studied for several decades now, mostly as a treatment for malignancies and hematological diseases but also for genetic metabolic disorders. Since many diseases that could be potentially treated with this approach develop early in life, studies of cell transplantation in newborn mice are needed, especially for gene therapy protocols. However, the small size of pups restricts the possibilities for routes of administration, and those available are normally technically challenging.

Hemodynamic effects of incremental doses of acepromazine in isoflurane-anesthetized dogs.

Objective: To evaluate the effects of incremental doses of acepromazine on hemodynamics in isoflurane-anesthetized dogs.

Study Design: Prospective, experimental study.

Animals: Healthy, adult, mixed-breed dogs (two male and four female) weighing 16.

Hemodynamic, respiratory and sedative effects of progressively increasing doses of acepromazine in conscious dogs.

Objective: To evaluate the effects of progressively increasing doses of acepromazine on cardiopulmonary variables and sedation in conscious dogs.

Study Design: Prospective, experimental study.

Animals: A group of six healthy, adult, mixed-breed dogs weighing 16.

Effects of 3 morphine doses, in combination with acepromazine, on sedation and some physiological parameters in dogs.

This study evaluated the effects of 3 morphine doses combined with acepromazine, on sedation and physiological parameters in 5 clinically healthy dogs. Four treatments were administered intramuscularly in a randomized, blinded, crossover design: acepromazine, 0.05 mg/kg, alone (ACP) and acepromazine plus morphine at doses of 0.

Adipose tissue derived mesenchymal stem cell transplantation in the treatment of ischemia/reperfusion induced acute kidney injury in rats. Application route and therapeutic window1.

Purpose: To evaluate renal repair in rats who had renal infarction induced by the obstruction of blood flow in the renal artery and were treated with transplantation of adipose tissue derived mesenchymal stem cell.

Methods: 16-week-old Wistar rats (n=72) were used, submitted to celiotomy and had of the renal artery and vein clipped for 24 hours. The animals were randomly assigned to 10 experimental homogeneous groups, corresponding to the treatments with phosphate-buffered saline (PBS) or adipose tissue derived mesenchymal stem cell (ADSC), duration of application (24 or 48 hours), and site of transplantation (lateral vein of the tail or intrarenal).

Combined effects of melatonin and topical hypothermia on renal ischemia-reperfusion injury in rats.

Purpose: To evaluate whether their combination was more effective than either alone in decreasing renal damage due to ischemia/reperfusion (I/R) injury in rats.

Methods: Thirty-two Wistar rats were assigned to four groups. Following right nephrectomy, their left kidneys were subjected to warm ischemia (IR), cold ischemia (TH+IR), intraperitoneal injection of 10 mg/kg melatonin (MEL+IR), or injection of 10 mg/kg melatonin followed by cold ischemia (MEL+TH+IR).

Sedative and cardiopulmonary effects of dexmedetomidine infusions randomly receiving, or not, butorphanol in standing horses.

Dexmedetomidine (DEX) alone, or combined with butorphanol (BUT), may be administered by constant rate infusions (CRIs) in standing horses. This blinded, randomised, crossover study in six healthy adult horses aimed to determine the sedative and cardiopulmonary effects of DEX (dexmedetomidine (3.5 µg/kg+5 µg/kg/hour CRI) and DEX/BUT (dexmedetomidine (3.

Effects of three methadone doses combined with acepromazine on sedation and some cardiopulmonary variables in dogs.

Objective: To evaluate the sedative and cardiopulmonary effects of three methadone doses, combined with acepromazine, in dogs.

Study Design: Prospective, randomized, complete block study.

Animals: Six healthy, adult, cross-bred dogs weighing 17.

Effects of a prolonged infusion of fentanyl, with or without atropine, on the minimum alveolar concentration of isoflurane in dogs.

Objectives: To evaluate the effect of a prolonged constant rate infusion (CRI) of fentanyl on the minimum alveolar concentration (MAC) of isoflurane (ISOMAC ) and to establish whether concurrent atropine administration influences ISOMAC in dogs.

Study Design: Prospective, crossover study.

Animals: Six healthy dogs weighing 13.

Effects of 8 hemodynamic conditions on direct blood pressure values obtained simultaneously from the carotid, femoral and dorsal pedal arteries in dogs.

Objectives: This study aimed to evaluate the effect of 8 hemodynamic conditions on blood pressure measurements taken from the carotid, femoral and dorsal pedal arteries of dogs.

Animals: Six healthy dogs.

Methods: During isoflurane anesthesia, catheters were introduced into the carotid, femoral and dorsal pedal arteries of dogs to allow simultaneous monitoring of direct blood pressure in each artery.

Effects of tramadol alone, in combination with meloxicam or dipyrone, on postoperative pain and the analgesic requirement in dogs undergoing unilateral mastectomy with or without ovariohysterectomy.

Objective: To compare the effects of tramadol alone, or in combination with dipyrone or meloxicam, on postoperative pain and analgesia requirement after unilateral mastectomy with or without ovariohysterectomy in dogs.

Study Design: Prospective, randomized, clinical study.

Animals: Twenty seven bitches undergoing unilateral mastectomy with or without ovariohysterectomy.

Effect of intraperitoneal or incisional bupivacaine on pain and the analgesic requirement after ovariohysterectomy in dogs.

Objective: To compare the effect of intraperitoneal (IP) or incisional (INC) bupivacaine on pain and the analgesic requirement after ovariohysterectomy in dogs.

Study Design: Prospective, randomized clinical study.

Animals: Thirty female dogs undergoing ovariohysterectomy (OHE).

Comparison of the effects of epidural or intravenous methadone on the minimum alveolar concentration of isoflurane in dogs.

The effects of epidural and intravenous (IV) methadone (0.5mg/kg) on the minimum alveolar concentration of isoflurane (ISO(MAC)) were compared in dogs. Six dogs (16.

Modular hybrid total hip arthroplasty. Experimental study in dogs.

Background: This prospective experimental study evaluated the surgical procedure and results of modular hybrid total hip arthroplasty in dogs.

Methods: Ten skeletally mature healthy mongrel dogs with weights varying between 19 and 27 kg were used. Cemented modular femoral stems and uncemented porous-coated acetabular cups were employed.

Hemodynamic effects in dogs anesthetized with isoflurane and remifentanil-isoflurane.

Objective: To compare hemodynamic effects in dogs anesthetized with remifentanilisoflurane and with isoflurane alone.

Animals: 6 adult dogs.

Procedures: Mechanically ventilated, isoflurane-anesthetized dogs received increasing constant rate infusions (CRIs) of remifentanil (0.

Effects of remifentanil on the minimum alveolar concentration of isoflurane in dogs.

Objective: To evaluate the effects of remifentanil on isoflurane minimum alveolar concentration (ISO(MAC)) in dogs.

Animals: 6 adult mixed-breed dogs.

Procedures: Dogs were anesthetized with isoflurane on 2 occasions.

Evaluation of cardiorespiratory effects of combinations of dexmedetomidine and atropine in cats.

The cardiovascular effects of dexmedetomidine alone or in combination with atropine were studied in six cats. Cats underwent four treatments in a randomized crossover design as follows: DEX15, saline+dexmedetomidine 15microg/kg; DEX30, saline+dexmedetomidine 30microg/kg; ADEX15, atropine+dexmedetomidine 15microg/kg; ADEX30, atropine+dexmedetomidine 30microg/kg. Pulse rate (PR) and systolic arterial pressure (SAP) decreased in DEX15 and DEX30.

Comparative study on the sedative effects of morphine, methadone, butorphanol or tramadol, in combination with acepromazine, in dogs.

Objective: To compare the effects of morphine (MOR), methadone (MET), butorphanol (BUT) and tramadol (TRA), in combination with acepromazine, on sedation, cardiorespiratory variables, body temperature and incidence of emesis in dogs.

Study Design: Prospective randomized, blinded, experimental trial.

Animals: Six adult mixed-breed male dogs weighing 12.

Assessment of brachial plexus blockade in chickens by an axillary approach.

Objective: To assess the brachial plexus block in chickens by an axillary approach and using a peripheral nerve stimulator.

Study Design: Prospective, randomized, double-blinded study.

Animals: Six, 84-week old, female chickens.

Effects of methadone, alone or in combination with acepromazine or xylazine, on sedation and physiologic values in dogs.

Objective: To evaluate the effects of methadone, administered alone or in combination with acepromazine or xylazine, on sedation and on physiologic values in dogs.

Study Design: Randomized cross-over design.

Animals: Six adult healthy mixed-breed dogs weighing 13.

Effects of epidural administration of dexmedetomidine on the minimum alveolar concentration of isoflurane in dogs.

Objective-To evaluate the effects of epidural administration of 3 doses of dexmedetomidine on isoflurane minimum alveolar concentration (MAC) and characterize changes in bispectral index (BIS) induced by nociceptive stimulation used for MAC determination in dogs. Animals-6 adult dogs. Procedures-Isoflurane-anesthetized dogs received physiologic saline (0.

Use of bispectral index to monitor depth of anesthesia in isoflurane-anesthetized dogs.

Objective: To evaluate the correlation between the bispectral index (BIS) and end-tidal isoflurane (ET(ISO)) concentration and compare the use of 3 BIS sensor positions in dogs.

Animals: 6 adult dogs.

Procedures: Mechanically ventilated dogs received pancuronium, and depth of anesthesia was altered by increasing ET(ISO) concentration from 1.