Molecular mechanism of YbtA-mediated transcriptional regulation of divergent overlapping promoters ybtA and irp6 of Yersinia enterocolitica.

Genes of Yersiniae spp. involved in production of the siderophore yersiniabactin are located on the high-pathogenicity island (HPI). Their transcription is controlled by the AraC/XilS-like transcriptional regulator YbtA encoded within the HPI.

Transcriptional regulation of high pathogenicity island iron uptake genes by YbtA.

A large group of Enterobacteriaceae, including members of the genus Yersinia, produce the extracellular siderophore yersiniabactin enabling them to multiply under iron-depleted conditions. Genes, involved in yersiniabactin synthesis, transport and regulation are clustered in the high pathogenicity island (HPI). YbtA, an AraC-like transcriptional regulator, is presumed to be the central regulator of yersiniabactin production together with the ferric uptake regulator Fur.

Irp9, encoded by the high-pathogenicity island of Yersinia enterocolitica, is able to convert chorismate into salicylate, the precursor of the siderophore yersiniabactin.

The Irp9 protein of Yersinia enterocolitica participates in the synthesis of salicylate, the precursor of the siderophore yersiniabactin. In Pseudomonas species, salicylate synthesis is mediated by two enzymes: isochorismate synthase and isochorismate pyruvate-lyase. Both enzymes are required for complementation of a Yersinia irp9 mutant.