Publications by authors named "Daniela Barretto Barbosa Trivella"

The Amazon, an important biodiversity hotspot, remains poorly explored in terms of its microbial diversity and biotechnological potential. The present study characterized the metabolic potential of Gram-positive strains of the Actinomycetes and Bacilli classes isolated from soil samples of an Amazon Conservation Unit. The sequencing of the 16S rRNA gene classified the strains ACT015, ACT016, and FIR094 within the genera , , and , respectively.

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Article Synopsis
  • - Untargeted metabolomics, often reliant on data-dependent acquisition (DDA), has limitations in fragmenting all detected ions and specificity, prompting the development of a dynamic procedure to enhance detection of biological signals.
  • - The study analyzed three species of actinomycetes using liquid chromatography and mass spectrometry, employing MZmine for preprocessing and a custom tool, RegFilter, for data processing.
  • - RegFilter improved ion detection by covering the whole chromatographic run and allowed for the annotation of biologically relevant candidates, thus serving as a valuable complement to DDA and enabling the creation of customized spectral libraries.
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  • The nucleocapsid (N) protein of coronaviruses is crucial for genome transcription and packaging, making it a prime focus for antiviral development.
  • A novel fluorescence polarization assay identified small molecules, notably L-chicoric acid (CA), that inhibit the N protein's binding to viral RNA.
  • CA was confirmed as a high-affinity ligand for the N protein and demonstrated effectiveness in reducing SARS-CoV-2 replication in cell cultures, highlighting potential new antiviral strategies.
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The nucleocapsid (N) protein of the SARS-CoV-2 virus, the causal agent of COVID-19, is a multifunction phosphoprotein that plays critical roles in the virus life cycle, including transcription and packaging of the viral RNA. To play such diverse roles, the N protein has two globular RNA-binding modules, the N- (NTD) and C-terminal (CTD) domains, which are connected by an intrinsically disordered region. Despite the wealth of structural data available for the isolated NTD and CTD, how these domains are arranged in the full-length protein and how the oligomerization of N influences its RNA-binding activity remains largely unclear.

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sp. BRA-346 is an Actinobacteria isolated from the Brazilian endemic tunicate sp. We have reported that this strain produces epoxyketone peptides, as dihydroeponemycin (DHE) and structurally related analogs.

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Bacteria of the genus are known for their psychrophilic characteristics, being extremophilic organisms capable of surviving and reproducing in hostile environments of low temperature and high pressure. Among many of the genus characteristics, there is the ability to produce enzymes and molecules of industrial biotechnology importance, such as pigments and proteins related to heavy metal bioremediation. The bacterium strain LAMA 639 was isolated from sediments from the Walvis Ridge ocean crest at a depth of 4.

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Technologies based on synthetic biology to produce bacterial natural carotenoids depend on information regarding their biosynthesis. Although the biosynthetic pathway of common carotenoids is known, there are carotenoids whose pathways are not completely described. This work aimed to mine the genome of the deep-sea bacterium Erythrobacter citreus LAMA 915, an uncommon bacterium that forms yellow colonies under cultivation.

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Bacterial genome sequencing has revealed a vast number of novel biosynthetic gene clusters (BGC) with potential to produce bioactive natural products. However, the biosynthesis of secondary metabolites by bacteria is often silenced under laboratory conditions, limiting the controlled expression of natural products. Here we describe an integrated methodology for the construction and screening of an elicited and pre-fractionated library of marine bacteria.

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Enterococci are gram-positive, widespread nosocomial pathogens that in recent years have developed resistance to various commonly employed antibiotics. Since finding new infection-control agents based on secondary metabolites from organisms has proved successful for decades, natural products are potentially useful sources of compounds with activity against enterococci. Herein are reported the results of a natural product library screening based on a whole-cell assay against a gram-positive model organism, which led to the isolation of a series of anacardic acids identified by analysis of their spectroscopic data and by chemical derivatizations.

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The IL-10 family of cytokines is comprised of IL-10, IL-19, IL-20, IL-22, IL-24, IL-26, and IFN-lambdas (IL-28A, IL-28B, and IL-29). The IL-10 family members bind to shared class II cytokine receptor chains that associate in various combinations in heterodimeric complexes. Upon interleukin/receptor complex formation, these proteins switch on the Jak/STAT pathway and elicit pleiotropic biological responses whose variety sharply contrasts with their structural similarities.

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