Multiplex qPCR for the detection of bacteria associated with huanglongbing ' Liberibacter asiaticus', '. L. americanus' and 16Sr IX group phytoplasma.

The occurrence of 'Candidatus Liberibacter' spp. and '. Phytoplasma' spp.

Incidence of Carrying Liberibacter asiaticus in Brazil's Citrus Belt.

Huanglongbing (HLB) is a citrus disease of worldwide importance, associated with the presence of Liberibacter asiaticus (Las) and vectored by the psyllid in Asia and the Americas. To properly manage HLB, removal of inoculum sources and control of the psyllid are undertaken. We evaluated the percentage of the psyllid population with Las, sampled from yellow sticky traps over a three-year period and its relationship with insect population, regions, season of the year, and HLB management in citrus areas in the southwestern, central, and northern regions of São Paulo (SP) and southwestern region of Minas Gerais states, Brazil.

Molecular Characterization and Detection of 16SrIII Group Phytoplasma Associated with Huanglongbing Symptoms.

When huanglongbing (HLB) was found in Brazil in 2004, 'Candidatus Liberibacter americanus' was infecting most of the trees while 'Ca. L. asiaticus' was present in a minor proportion.