Novel strategies of glutathione depletion in photodynamic and chemodynamic therapy: A review.

Cancer remains a health problem worldwide; therefore, developing new therapies to increase the effectiveness of anticancer treatments is necessary. Two such methods are photodynamic therapy (PDT) and chemodynamic therapy (CDT). The intensive growth and increased metabolism of tumors lead to elevated levels of reactive oxygen species (ROS) within cancer cells.

Risk Factors for Non-Healing Wounds-A Single-Centre Study.

Chronic wounds present a significant clinical, social, and economic challenge. This study aimed to objectify the risk factors of healing outcomes and the duration of chronic wounds from various etiologies. Patients treated for non-healing wounds at the surgical outpatient clinic of the Olomouc Military Hospital were involved.

Nonwoven Releasing Propolis as a Potential New Wound Healing Method-A Review.

Wound healing poses a serious therapeutic problem. Methods which accelerate tissue regeneration and minimize or eliminate complications are constantly being sought. This paper is aimed at evaluation of the potential use of biodegradable polymer nonwovens releasing propolis as wound healing dressings, based on the literature data.

THE EFFECT OF SULFASALAZINE AND 5-AMINOSALICYLIC ACID ON THE SECRETION OF INTERLEUKIN 8 BY HUMAN COLON MYOFIBROBLASTS.

Sulfasalazine (SAS) and its therapeutically active derivative--5-aminosalicylic acid (5-ASA) are used in the treatment of inflammatory bowel disease. 5-ASA mechanism of action on the one hand, involves the inhibition of the cyclooxygenase and lipoxygenase activity, and thus decrease of synthesis of prostaglandins, leukotrienes and free radicals, on the other hand, the suppression of the immune response in the intestinal mucosa. Myofibroblasts, which are located just below the basement membrane, are important element of the mucosa.

Antiproliferative effect of valproic acid and 5,7-dimethoxycoumarin against A2058 human melanoma cells.

Melanoma is one of the most malignant tumors of a dangerous high incidence and high metastatic potential. It grows quickly and in an advanced stage is resistant to radio-, chemo- and immunotherapy, which makes it difficult to cure. Therefore, research efforts are focused on the development of new therapeutics or chemopreventive strategies.

The sugar 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) as a characteristic component of bacterial endotoxin -- a review of its biosynthesis, function, and placement in the lipopolysaccharide core.

The sugar 3-deoxy-d-manno-oct-2-ulosonic acid (Kdo) is a characteristic component of bacterial lipopolysaccharide (LPS, endotoxin). It connects the carbohydrate part of LPS with C6 of glucosamine or 2,3-diaminoglucose of lipid A by acid-labile α-ketosidic linkage. The number of Kdo units present in LPS, the way they are connected, and the occurrence of other substituents (P, PEtn, PPEtn, Gal, or β-l-Ara4N) account for structural diversity of the inner core region of endotoxin.

Comparison of dissolution profiles of theophylline extended-release dosage forms.

Dissolution testing is a very important tool used to demonstrate the similarity between different formulations. Due to a narrow therapeutic range of theophylline, it is crucial to investigate the differences in the rate of release of this drug between the products. The aim of study was to compare the dissolution profiles of theophylline extended-release dosage forms available on Polish market: Theoplus, Theovent, Theospirex retard, and Euphyllin long.

Effects of 300 mT static magnetic field on IL-6 secretion in normal human colon myofibroblasts.

Intestinal subepithelial myofibroblasts play crucial role in the growth and development of the intestine. Colitis, small bowel injury, gastric ulcer disease and inflammatory bowel disease (IBD) accompany the increase of number of activated myofibroblasts. In the last few years, the increasing production of electromagnetic (EMF) and static magnetic fields (SMF), due to the expanding use of electronic devices in everyday life, has led to a number of studies on the effects of these fields on living organisms.

The chemical composition of endotoxin isolated from intestinal strain of Desulfovibrio desulfuricans.

Desulfovibrio desulfuricans anaerobes are constituents of human alimentary tract microflora. There are suggestions that they take part in the pathogenesis of periodontitis and some gastrointestinal inflammatory disorders, such as ulcerative colitis or Crohn's disease. Endotoxin is one of Gram-negative bacteria cellular components that influence these microorganisms pathogenicity.

The pyrolytic profile of lyophilized and deep-frozen compact part of the human bone.

Background. Bone grafts are used in the treatment of nonunion of fractures, bone tumors and in arthroplasty. Tissues preserved by lyophilization or deep freezing are used as implants nowadays.

Chemical composition of Desulfovibrio desulfuricans lipid A.

Lipopolysaccharides also called endotoxins are an integral component of the outer membrane of Gram-negative bacteria. When released from the bacterial surface, they interact with a host immune system, triggering excessive inflammatory response. Lipid A is the biologically most active part of endotoxin, and its activity is modulated by the quantity, quality and arrangement of its fatty acids.

The role of Desulfovibrio desulfuricans lipopolysaccharides in modulation of periodontal inflammation through stimulation of human gingival fibroblasts.

Objective: Periodontitis is a destructive disease which is likely to be the result of the activities of different microbial complexes. Recently, sulphate-reducing bacteria (SRB) have been detected in the oral cavity, and they have been found to be common inhabitants of sites showing periodontal destruction. The aim of study was to evaluate the influence of endotoxins of Desulfovibrio desulfuricans bacteria on human gingival fibroblast HGF-1 line.

Interstrain diversity of 2-keto-3-deoxyoctonate content in lipopolysaccharides of Desulfovibrio desulfuricans.

Bacteria of Desulfovibrio desulfuricans species are Gram-negative, anaerobic rods selectively reducing sulphates and colonizing oxygen-free ecosystems. They are ubiquitous in the natural environment and have been also found to reside in the human digestive tract. They are suggested to be involved in the pathogenesis ofulcerative colitis and Crohn's disease.

[The structural diversity of lipid A from gram-negative bacteria].

The majority of Gram-negative bacteria are pathogenic to humans and animals. Lipopolysaccharide (LPS) is the most biologically active component of these microorganisms. This compound is also called endotoxin to emphasize its negative impact on a macroorganism.

[Methods of lipopolysaccharide component derivation in evaluating their structures by chromatographic techniques].

The pathogenicity of Gram-negative bacteria is mediated mainly by the outer membrane-associated lipid-polysaccharide structure, called endotoxin or lipopolysaccharide (LPS). This structure consists of three parts, O-antigen, core oligosaccharide, and lipid A, which differ in chemical composition and biological properties. Gas-liquid chromatography coupled with mass spectrometry (GLC/MS) is commonly used to determine LPS structure.