Publications by authors named "Daniel Wegmueller"
Nucleic Acids Res
June 2004
Article Synopsis
- HT1080 cells expressing GFP linked to AU-rich elements serve as a model to study mRNA stability by tracking fluorescence intensity changes.
- The study investigates how small interfering RNAs (siRNAs) targeting AU-binding proteins (AUBPs) impact mRNA stability, revealing that targeting HuR or BRF1 affects fluorescence levels, indicating their roles as stabilizers or destabilizers.
- Interestingly, selectively reducing certain AUF1 isoforms (p40AUF1/p45AUF1) significantly increases fluorescence and stabilizes mRNA, suggesting that the specific isoform levels, not just the total AUF1 amount, influence mRNA stability in these cells.
