Publications by authors named "Daniel Ruiz Buck"

Objectives: Analyse alternative methods of intrathecal antibody detection by comparing chemiluminescent immunoassay (CLIA) and enzyme-linked immunosorbent assay (ELISA) techniques to determine if CLIA can replace ELISA in the diagnosis of CNS infections.

Methods: A panel of 280 paired samples-cerebrospinal fluid (CSF) and serum-with known antibody reactivities (Varicella, n = 60; Measles, n = 120) and negative samples (n = 100) were used to evaluate the performance of six serological test kits (Enzygnost, VirClia®, and Serion ELISA (Measles and Variella).

Results: For Measles virus IgG, the VirClia® IgG monotest revealed 97% and 94% positive and negative agreement to the Enzygnost as reference test, respectively.

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Article Synopsis
  • - The study examines how microorganisms within plants can enhance their growth and health, focusing on the genetic diversity of these microbes, which is not well understood so far.
  • - Using advanced techniques, the research found that fungal infections in plant roots increased certain beneficial microorganisms and their related genes, which play a role in protecting the plants.
  • - By reconstructing specific genomes, researchers created a microbial mix that effectively suppressed root diseases, identifying a crucial gene cluster linked to the production of protective compounds in the plant microbiome.
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Pancreatic islet transplantation is a promising treatment for type 1 diabetes mellitus offering improved glycaemic control by restoring insulin production. Improved human pancreatic islet isolation has led to higher islet transplantation success. However, as many as 50% of islets are lost after transplantation due to immune responses and cellular injury, gene therapy presents a novel strategy to protect pancreatic islets for improved survival post-transplantation.

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