Capturing a rhodopsin receptor signalling cascade across a native membrane.

G protein-coupled receptors (GPCRs) are cell-surface receptors that respond to various stimuli to induce signalling pathways across cell membranes. Recent progress has yielded atomic structures of key intermediates and roles for lipids in signalling. However, capturing signalling events of a wild-type receptor in real time, across a native membrane to its downstream effectors, has remained elusive.

NaViA: a program for the visual analysis of complex mass spectra.

Motivation: Native mass spectrometry is now a well-established method for the investigation of protein complexes, specifically their subunit stoichiometry and ligand binding properties. Recent advances allowing the analysis of complex mixtures lead to an increasing diversity and complexity in the spectra obtained. These spectra can be time-consuming to tackle through manual assignment and challenging for automated approaches.

From Bench to Biomolecular Simulation: Phospholipid Modulation of Potassium Channels.

Potassium (K) ion channels are crucial in numerous cellular processes as they hyperpolarise a cell through K conductance, returning a cell to its resting potential. K channel mutations result in multiple clinical complications such as arrhythmia, neonatal diabetes and migraines. Since 1995, the regulation of K channels by phospholipids has been heavily studied using a range of interdisciplinary methods such as cellular electrophysiology, structural biology and computational modelling.