Sugar Detection in Mosquitoes via Anthrone Tests.

Adult mosquitoes feed on plant sugar, frequently in the form of floral nectar. However, because of spatial and temporal variation in this behavior, as well as to the predilection of most mosquitoes to alter their behavior in the presence of a researcher, observing mosquito nectar feeding and similar behaviors directly in real time is not always feasible. In this protocol, I describe methods for hot and cold anthrone tests, which can be used to quantify the degree to which mosquito sugar feeding occurs in nature.

Measuring Mosquito Spectral Sensitivity Using Electroretinograms.

Mosquitoes use a variety of cues to locate resources in the world around them, including olfactory, thermal, and visual stimuli. Understanding how mosquitoes perceive these stimuli is important for understanding and investigating mosquito behaviors and mosquito ecology. Specifically, mosquito vision can be studied in many ways, including by using electrophysiological recordings from their compound eyes.

Mosquito (Diptera: Culicidae) Vision and Associated Electrophysiological Techniques.

Mosquitoes are considered the world's deadliest animal because of the pathogens they spread. Additionally, they are an unbearable nuisance in many areas. Visual stimuli play an important role in the mosquito life cycle, helping them find vertebrate hosts, floral nectar, and oviposition sites.

Mosquito Pollination and Sugar Detection Methods: An Overview.

The interactions between mosquitoes and plants and, particularly, between mosquitoes and plant sugars from flowers and other structures are often overlooked and are vastly underresearched compared to mosquito-vertebrate or mosquito-pathogen interactions. Given the importance of mosquito nectar-feeding behavior, as well as its impact on vectorial capacity and its implications for vector suppression, a better understanding of mosquito-plant interactions is needed. Direct observation of mosquitoes visiting plants to obtain sugar and other nutrients can be difficult because females may leave flowers to seek a blood meal from the observer, but this can be overcome with the right experimental procedures.

Assessment of Mosquito Pollination.

Adult mosquitoes, sometimes in vast numbers, visit flowers in their search for floral nectar. However, the ability of mosquitoes to pollinate the flowers they visit is often neglected and sometimes even presumptively dismissed. Despite this, mosquito pollination has been reported in many instances, although many questions linger about its extent, importance, and the breadth of floral species or mosquito species which may be involved.

The Invasive Mosquitoes of Canada: An Entomological, Medical, and Veterinary Review.

Several invasive mosquitoes have become established in Canada, including important pathogen vectors such as Aedes albopictus, Ae. japonicus, and Culex pipiens. Some species have been present for decades, while others are recent arrivals.

Sensory mechanisms for the shift from phytophagy to haematophagy in mosquitoes.

The Culicomorpha are an infraorder of several families of blood-feeding flies, including mosquitoes (Diptera: Culicidae). Here we discuss the evolutionary origins of blood-feeding within the Culicomorpha and review literature that suggests this behaviour may have evolved from ancestral plant-feeding or a combination of plant-feeding and insect-feeding. Sialomic and life-history evidence suggest that plant-feeding, concurrent or not with insect-feeding, is parsimonious as an ancestral diet for Culicomorpha.

Cheese and cheese infusions: ecological traps for mosquitoes and spotted wing Drosophila.

Background: Harnessing insect ecology for insect control is an innovative concept that seeks to exploit, among others, insect-microbe ecological interactions for improved control of pest insects. Microbe-produced cheese odour attracts several dipterans, including host-seeking mosquitoes, but this phenomenon has not been thoroughly explored for mosquito control. Here we tested the hypothesis that attraction of mosquitoes to cheese odour can be exploited as an ecological trap for mosquito control.

An Emergency Department Clinical Algorithm to Increase Early Palliative Care Consultation: Pilot Project.

The emergency department (ED) is a primary entry point of hospitals but does not have a system to identify and consult palliative care (PC) early in patients who meet criteria. To determine the measurable effects of an ED PC consultation on patients who meet criteria, hypothesizing that ED PC consultation would lead to decreased average length of stay (ALOS), average direct cost per patient, decreased number of surgeries, and radiological tests performed per patient. A physician-led data-driven evidence-based algorithm was designed and piloted with implementation in two hospitals during January-March 2019 in Orlando, FL.

Molecular relationships of introduced Aedes japonicus (Diptera: Culicidae) populations in British Columbia, Canada using mitochondrial DNA.

Aedes japonicus japonicus (Theobald) is a relatively recent immigrant to the Pacific Northwest, having been collected in Washington State in 2001 and in British Columbia (BC) since 2014. We applied a molecular barcoding approach to determine the phylogenetic relationship of Ae. j.

Modeling the Putative Ancient Distribution of Aedes togoi (Diptera: Culicidae).

The coastal rock pool mosquito, Aedes (Tanakius) togoi (Theobald) (Diptera: Culicidae), is found in coastal east Asia in climates ranging from subtropical to subarctic. However, a disjunct population in the Pacific Northwest of North America has an ambiguous heritage. Two potential models explain the presence of Ae.

Clinical Transformation Through Change Management Case Study: Chest Pain in the Emergency Department.

Introduction/background: Adults with chest pain presenting to an emergency department are high-risk and high-volume. A methodology which gathers practicing physicians together to review evidence and share practice experience to formulate a written algorithm with key decision points and measures is discussed with implementation, based on change management principles, and results.

Methods: A methodology was followed to "establish the standard-of-care".

Ultraviolet inflorescence cues enhance attractiveness of inflorescence odour to Culex pipiens mosquitoes.

Inflorescence patterns of ultraviolet (UV) absorption and UV-reflection are attractive to many insect pollinators. To understand whether UV inflorescence cues affect the attraction of nectar-foraging mosquitoes, we worked with the common house mosquito, Culex pipiens and with two plant species exhibiting floral UV cues: the tansy, Tanacetum vulgare, and the common hawkweed Hieraciumm lachenalii. Electroretinograms revealed that Cx.

Lemongrass and Cinnamon Bark: Plant Essential Oil Blend as a Spatial Repellent for Mosquitoes in a Field Setting.

Plant essential oils (EOs) have been considered as spatial repellents to help disrupt the pathogen transmission cycle of mosquitoes. Our objective was to assess spatial repellency effects of EOs on the tropical yellow fever mosquito, Aedes aegypti (L.) (Diptera: Culicidae) and on local mosquito populations in coastal British Columbia (Canada).

Modeled distributions of Aedes japonicus japonicus and Aedes togoi (Diptera: Culicidae) in the United States, Canada, and northern Latin America.

The Asian bush mosquito, Aedes japonicus japonicus, and the coastal rock pool mosquito, Aedes togoi, are potential disease vectors present in both East Asia and North America. While their ranges are fairly well-documented in Asia, this is not the case for North America. We used maximum entropy modeling to estimate the potential distributions of Ae.

Author Correction: Multimodal floral cues guide mosquitoes to tansy inflorescences.

A correction to this article has been published and is linked from the HTML and PDF versions of this paper. The error has been fixed in the paper.

Multimodal floral cues guide mosquitoes to tansy inflorescences.

Female mosquitoes exploit olfactory, CO, visual, and thermal cues to locate vertebrate hosts. Male and female mosquitoes also consume floral nectar that provides essential energy for flight and survival. Heretofore, nectar-foraging mosquitoes were thought to be guided solely by floral odorants.

Attraction of Female (L.) to Aphid Honeydew.

Plant sugar is an essential dietary constituent for mosquitoes, and hemipteran honeydew is one of the many forms of plant sugar that is important to mosquitoes. Many insects rely on volatile honeydew semiochemicals to locate aphids or honeydew itself. Mosquitoes exploit volatile semiochemicals to locate sources of plant sugar but their attraction to honeydew has not previously been investigated.

Populations of the gall midge Dasineura oxycoccana on cranberry and blueberry produce and respond to different sex pheromones.

We identified and field-tested the sex pheromones of Dasineura oxycoccana (Johnson) (Diptera: Cecidomyiidae) midges collected from cranberry, Vaccinium macrocarpon Aiton, and from highbush blueberry, Vaccinium corymbosum L., commonly named cranberry tipworm (CTW) and blueberry gall midge (BGM), respectively. Coupled gas chromatographic-electroantennographic detection (GC-EAD) analyses of pheromone gland extract from the ovipositor of calling CTW females revealed one component (<10 pg per ovipositor/pheromone gland) that elicited antennal responses from CTW males.