Publications by authors named "Daniel N El Kodsi"
Article Synopsis
- Single-cell RNA sequencing (scRNA-seq) is a valuable technique for understanding how gene variations affect RNA expression in individual cells, and allele-specific expression (ASE) analysis enhances this understanding by focusing on genetic differences at the RNA level.* -
- The study reveals that using single-nucleus RNA-seq (snRNA-seq) can improve analysis of ASE due to increased intronic reads, which are more likely to contain genetic variants, and demonstrates how experimental factors like RNA source and sequencing depth can influence the effectiveness of these analyses.* -
- The researchers developed new computational tools and compared their ASE methods against traditional expression quantitative trait locus (eQTL) analysis in a Parkinson's disease study, finding that ASE analysis was
Parkin coregulates glutathione metabolism in adult mammalian brain.
Acta Neuropathol Commun
January 2023
We recently discovered that the expression of PRKN, a young-onset Parkinson disease-linked gene, confers redox homeostasis. To further examine the protective effects of parkin in an oxidative stress model, we first combined the loss of prkn with Sod2 haploinsufficiency in mice. Although adult prkn//Sod2 animals did not develop dopamine cell loss in the S.
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- The study investigates how parkin, a protein, protects the brain from Parkinson's disease, particularly focusing on its cysteine residues that undergo redox reactions and posttranslational modifications.* -
- Research findings reveal that aging leads to parkin becoming largely insoluble due to oxidation, particularly at specific cysteine residues, and this results in increased levels of harmful hydrogen peroxide (HO) in both mice and parkin-deficient human brains.* -
- The protective effects of wild-type parkin against dopamine toxicity are emphasized, as it reduces HO levels and neutralizes reactive dopamine metabolites, while disease-linked parkin mutants do not exhibit these protective characteristics.*