Publications by authors named "Daniel M Bornman"
Article Synopsis
- Human saliva can reveal important information about both oral and overall health; this study analyzed saliva samples from healthy individuals and compared them to datasets in the Human Microbiome Project.
- Using the GENIUS system, researchers successfully identified over 175 bacterial species in saliva samples, achieving high accuracy and speed in their results.
- While both GENIUS and BLASTn analyses found major bacterial groups, GENIUS proved to be more precise in species identification and strain-level details, significantly outperforming other methods.
Short-read, high-throughput sequencing technology for STR genotyping.
Biotech Rapid Dispatches
April 2012
Article Synopsis
- DNA identification mainly uses short tandem repeat (STR) genotyping but traditional methods have low throughput and lack sequence information.
- High-throughput sequencing technology holds potential for better accuracy in human identification but is not yet validated for forensic use.
- This study outlines a method using high-throughput sequencing to analyze CODIS STR loci, showing it can accurately genotype individuals and mixtures, requiring only 18,500 reads for reliable results while also identifying SNPs and quantifying mixed samples.
Background: Decreased numbers of Langerhans cells (LCs) in the cervix of human immunodeficiency virus (HIV)-infected women are believed to contribute to the progression of human papilloma virus (HPV)-related squamous intraepithelial lesions. However, this impairment of local immunity has not been well studied in the vulva. The objective of this study was to compare the S100+ LC density in high-grade vulvar intraepithelial neoplasia (VIN) in HIV-positive and HIV-negative women.
View Article and Find Full Text PDFYersinia pestis is one of the most threatening biological agents due to the associated high mortality and history of plague pandemics. Identifying molecular players in the host response to infection may enable the development of medical countermeasures against Y. pestis.
View Article and Find Full Text PDFActivation of fatty acid synthase (FAS) expression and fatty acid synthesis is a common event in human breast cancer. Sterol regulatory element binding proteins (SREBPs) are a family of transcription factors that regulate genes involved in lipid metabolism, including FAS. SREBP-1c expression is induced in liver and adipose tissue by insulin and by fasting/refeeding and is critical for nutritional regulation of lipogenic gene expression.
View Article and Find Full Text PDFMucinous cancers of the breast are distinguished histologically by their abundant pools of mucin and low degree of nuclear pleomorphism. Relative to the more common breast cancers of no distinctive type (ductal carcinoma), mucinous cancers have a relatively favorable prognosis. In a study of chromosomal changes in mucinous cancers, we evaluated the extent of loss of heterozygosity (LOH) at chromosomal regions commonly deleted in usual infiltrating ductal carcinoma, including markers on chromosomal arms 1p, 1q, 3p, 6q, 8p, 9p, 11p, 11q, 13q, 16q, 17p, and 17q.
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